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Adsorption of Amelogenin Nanopheres onto Charged Surfaces, A Model for Tooth Enamel Matrix Re-construction

Published online by Cambridge University Press:  17 March 2011

Moradian-Oldak J. ,
Gergely C. ,
Bouropoulos N.  and
Cuisinier F.J.G.
Moradian-Oldak J.
Affiliation:
Center for Craniofacial Molecular Biology, University of Southern California, LA, CA, USA
Gergely C.
Affiliation:
INSERM U 595, Faculté de Chirurgie Dentaire, Université Louis Pasteur, Strasbourg, France
Bouropoulos N.
Affiliation:
Department of Material Science, University of Patras, GR-26500 Patras, Greece
Cuisinier F.J.G.
Affiliation:
INSERM U 595, Faculté de Chirurgie Dentaire, Université Louis Pasteur, Strasbourg, France
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Abstract

Amelogenins are hydrophobic proteins that constitute more than 90% of the secretory stage enamel matrix. The assembly of amelogenin into nanospheres has been postulated to be a key factor in controlling the structural organization of the enamel extracellular matrix framework, which provides the scaffolding for the elongated and oriented growth of enamel apatite crystals. To get insight into the structure and function of amelogenin in controlling the process of crystal growth we have utilized two different approaches to investigate adsorption of amelogenin nanospheres onto charged surfaces: A) analysis of adsorption of amelogenin onto hydroxyapatite crystals by means of Langmuir Model for protein adsorption. B) analysis of amelogenin mono or multi-layer formation by sequential adsorption process onto auto-assembled polyelctrolytes films. Our data indicate that amelogenin nanospheres adsorb onto the surface of apatite crystals as binding units with defined adsorption sites. We found that amelogenin nanospheres are negatively charged and a monolayer of these nanospheres adsorbed in an irreversible way on positively ending polyelectrolyte multilayers most likely through electrostatic interactions.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 823: Symposium W – Biological and Bioinspired Materials and Devices , 2004 , W6.3
Copyright
Copyright © Materials Research Society 2004

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