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Adhesion of Soft Biological Shells controlled by Bending Elasticity and Macromolecular Networks

Published online by Cambridge University Press:  10 February 2011

R. Simson ,
A. Albersdörfer  and
E. Sackmann
R. Simson
Affiliation:
Physik Department E22, Technische Universität München, James Franck Strasse, 85748 Garching, Germany. sackmann@physik.tu-muenchen.de
A. Albersdörfer
Affiliation:
Physik Department E22, Technische Universität München, James Franck Strasse, 85748 Garching, Germany. sackmann@physik.tu-muenchen.de
E. Sackmann
Affiliation:
Physik Department E22, Technische Universität München, James Franck Strasse, 85748 Garching, Germany. sackmann@physik.tu-muenchen.de
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Abstract

We present an interferometrie technique allowing reliable measurements of bending modulus κ, membrane tension Σ and adhesion energy W of cells crawling on substrates. All three parameters are important for cell locomotion and reflect the local balance of attractive and repulsive forces between cell and substratum as well as the internal coupling of cell membrane and the underlying cytoskeleton. Mutants of the Dictyostelium ameba lacking an important cytoskeletal protein, Cortexillin, exhibited a markedly reduced bending modulus and adhesion energy as compared to wild type Dictyostelium. In addition, experiments with model membrane systems suggest that the combination of attractive and repulsive forces results in a local clustering of receptors mediating cell adhesion.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 463: Symposia EE – Statistical Mechanics in Physics and Biology , 1996 , 3
Copyright
Copyright © Materials Research Society 1997

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References

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