Hostname: page-component-669899f699-7xsfk Total loading time: 0 Render date: 2025-04-26T13:16:22.542Z Has data issue: false hasContentIssue false
  • English
  • Français

Fabrication and Characterisation of Calcium Phosphate - Liposome Composites as an Implant Coating

Published online by Cambridge University Press:  15 March 2011

Tarinee Pongsaanutin  and
Jan T. Czernuszka
Tarinee Pongsaanutin
Affiliation:
Department of Materials, University of Oxford, Oxford, OX1 3PH, UK
Jan T. Czernuszka
Affiliation:
Department of Materials, University of Oxford, Oxford, OX1 3PH, UK
Get access

Abstract

Calcium phosphate in the form of apatite has been successfully precipitated on the surface of liposomes. Liposome vesicles were prepared by sonication of phosphatidylcholine and this was introduced into an aqueous solution of calcium and phosphate ions supersaturated with respect to hydroxyapatite. Calcium phosphate was shown to precipitate solely on the outer layer surface of the liposome vesicles. These composite assemblies were then deposited onto a stainless steel cathode substrate using an electrophoretic method at physiological temperatures.

Scanning electron microscopy (SEM), transmission electron microscopy (TEM), powder xray diffraction (PXRD) and Fourier transform infrared spectroscopy (FTIR) were used to analyse the morphology, structure and chemical composition of the composite coating. The results from PXRD and FTIR show a mixture of amorphous and poor crystalline hydroxyapatite (HAp). This was verified by electron diffraction. Dark field images confirmed that the precipitated HAp deposited solely at the outer surface of the liposomes. SEM micrographs demonstrated a thin uniform coating at the microstructure level. These results suggest that these calcium phosphateliposome composites can be formed. They have tremendous potential for use as drug delivery devices in aiding the treatments of bone disorders.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 662: Symposia LL/MM/NN/OO – Biomaterials for Drug Delivery & Tissue Engineering , 2000 , LL4.7
Copyright
Copyright © Materials Research Society 2001

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

1. Elliott, J.C., Structure and Chemistry of the Apatites and Other Calcium Orthophosphates, (Elsevier, 1994) pp.186–190.Google Scholar
2. Geros, R.Z. Le, Hydroxyapatite and Related Materials, ed. Brown, P.W. and Constantz, B., (CRC Press, 1994) pp. 3–28.Google Scholar
3. Illum, L., Novel Drug Delivery and Its Therapeutic Application, ed. Prescott, L.F. and Nimmo, W.S., (John Wiley & Sons, 1989), pp. 273–279.Google Scholar
4. Eanes, E.D., Bone and Mineral, 17, 269–272 (1992).Google Scholar
5. New, R.R.C., Liposome: a Practical approach, (Oxford University Press, 1997).Google Scholar
6. Peaker, A.J.S. and Czernuszka, J.T., Thin Solid Films, 287,174–183 (1996).Google Scholar
7. Lerner, E., Azoury, R. and Sarig, S., J. Cryst. Growth, 97, 725–730 (1989).Google Scholar
8. Holt, C., Kemennade, M.J.J.M. van, Harries, J.E., Nelson, L.S., Bailey, R.T., Hukins, D.W.L., Hasnain, S.S. and Bruyn, P.L. de, J. Cryst. Growth, 92, 239–252 (1998).Google Scholar
9. Gonzalez-Diaz, P.F. and Hidalgo, A., Spectrochimica Acta, 32A, 631–635 (1976).Google Scholar
10. Ishikawa, K., Ducheyne, P., Radin, S., Journal of Materials Science: Materials in Medicine, 4, 165–168 (1993).Google Scholar