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Peptide-mediated binding of gold nanoparticles to E. coli for enhanced microbial fuel cell power generation

Published online by Cambridge University Press:  20 June 2019

Justin P. Jahnke ,
Hong Dong ,
Deborah A. Sarkes ,
James J. Sumner ,
Dimitra N. Stratis-Cullum  and
Margaret M. Hurley
Justin P. Jahnke*
Affiliation:
Army Research Laboratory, 2800 Powder Mill Road, Adelphi, MD 20783, USA
Hong Dong
Affiliation:
Army Research Laboratory, 2800 Powder Mill Road, Adelphi, MD 20783, USA
Deborah A. Sarkes
Affiliation:
Army Research Laboratory, 2800 Powder Mill Road, Adelphi, MD 20783, USA
James J. Sumner
Affiliation:
Army Research Laboratory, 2800 Powder Mill Road, Adelphi, MD 20783, USA
Dimitra N. Stratis-Cullum
Affiliation:
Army Research Laboratory, 2800 Powder Mill Road, Adelphi, MD 20783, USA
Margaret M. Hurley
Affiliation:
Army Research Laboratory, 2800 Powder Mill Road, Adelphi, MD 20783, USA
*
Address all correspondence to Justin P. Jahnke at justin.p.jahnke.civ@mail.mil
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Abstract

The authors demonstrate that gold-binding peptides displayed on the outer membrane of Escherichia coli enhance bioelectrochemical charge transfer by binding gold nanoparticles. Microbial fuel cells were run with different gold-binding peptides displayed and with different nanoparticle sizes, and the results were correlated with transmission electron microscopy (TEM) imaging of nanoparticle binding. When a gold-binding peptide is displayed and 5 nm gold nanoparticles are present, up to 4× power generation over E. coli not displaying a gold-binding peptide is observed. While an enhanced current is observed using the previously published M6G9, the largest enhancement is observed when a new longer peptide named M9G18 is used.

Type
Research Letters
Information
MRS Communications , Volume 9 , Issue 3 , September 2019 , pp. 904 - 909
Copyright
Copyright © Materials Research Society 2019 

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