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Nanostructured substrates for multi-cue investigations of single cells

Published online by Cambridge University Press:  30 January 2018

Joseph A. Christodoulides ,
Marc Christophersen ,
Jinny L. Liu ,
James B. Delehanty ,
Deepa Raghu ,
Michael Robitaille ,
Jeff M. Byers  and
Marc P. Raphael
Joseph A. Christodoulides
Affiliation:
Naval Research Laboratory, 4555 Overlook Ave SW, Washington, DC 20375-5320, USA
Marc Christophersen
Affiliation:
Naval Research Laboratory, 4555 Overlook Ave SW, Washington, DC 20375-5320, USA
Jinny L. Liu
Affiliation:
Naval Research Laboratory, 4555 Overlook Ave SW, Washington, DC 20375-5320, USA
James B. Delehanty
Affiliation:
Naval Research Laboratory, 4555 Overlook Ave SW, Washington, DC 20375-5320, USA
Deepa Raghu
Affiliation:
Naval Research Laboratory, 4555 Overlook Ave SW, Washington, DC 20375-5320, USA Current Address: BioReliance, Sigma-Aldrich Corp., Rockville, MD 20850, USA
Michael Robitaille
Affiliation:
Naval Research Laboratory, 4555 Overlook Ave SW, Washington, DC 20375-5320, USA
Jeff M. Byers
Affiliation:
Naval Research Laboratory, 4555 Overlook Ave SW, Washington, DC 20375-5320, USA
Marc P. Raphael*
Affiliation:
Naval Research Laboratory, 4555 Overlook Ave SW, Washington, DC 20375-5320, USA
*
Address all correspondence to Marc P. Raphael atmarc.raphael@nrl.navy.mil
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Abstract

Cellular adhesion depends on the integration of numerous signaling inputs generated by the chemical and physical properties of the substrate. The complex coupling among inputs makes it challenging experimentally to deconvolve their individual contributions to the adhesion process. To address this roadblock, we have employed a combination of electron beam and optical lithographic techniques to fabricate substrates with independently tunable topographical and chemical signaling cues. Arrays of gold nanostructures were patterned atop quartz substrates, half of which were etched into gold-capped nanopillars. Individual A549 cells exposed simultaneously to Arg-Gly-Asp-functionalized etched and non-etched arrays exhibited strongly preferential adherence to the nanopillars.

Type
Research Letters
Information
MRS Communications , Volume 8 , Issue 1 , March 2018 , pp. 49 - 58
Copyright
Copyright © Materials Research Society 2018 

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