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3D Printed porous tissue engineering scaffolds with the self-folding ability and controlled release of growth factor

Published online by Cambridge University Press:  04 September 2020

Jiahui Lai ,
Junzhi Li  and
Min Wang
Jiahui Lai
Affiliation:
Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong
Junzhi Li
Affiliation:
Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong
Min Wang*
Affiliation:
Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong
*
Address all correspondence to Min Wang at memwang@hku.hk
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Abstract

This study investigated a new strategy for fabricating porous scaffolds with the self-folding ability and controlled release of growth factors (GFs) via 3D printing. The scaffolds were a bilayer structure comprising a poly(D,L-lactide-co-trimethylene carbonate) scaffold for providing the shape morphing ability and a gelatin methacrylate scaffold for encapsulating and delivering GF. The structure, shape morphing behavior, GF release, and its effect on stem cell behavior were studied for new scaffolds. The results suggest that these scaffolds have great potential for regenerating tissues such as blood vessels. This work also contributes to developments of 3D printing in tissue engineering.

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Type
Research Letters
Information
MRS Communications , Volume 10 , Issue 4 , December 2020 , pp. 579 - 586
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Copyright
Copyright © The Author(s), 2020, published on behalf of Materials Research Society by Cambridge University Press

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References

Wang, C., Zhao, Q., and Wang, M.: Cryogenic 3D printing for producing hierarchical porous and rhBMP-2-loaded Ca-P/PLLA nanocomposite scaffolds for bone tissue engineering. Biofabrication 9, 025031 (2017).CrossRefGoogle ScholarPubMed
Duan, B. and Wang, M.: Chapter 4 Selective laser sintering and its biomedical applications. In LaserTechnology in Biomimetics – Basics and Applications, edited by V. Schmidt (Springer, Berlin, 2013), pp. 83–109. ISBN 978-3-642-41340-7.CrossRefGoogle Scholar
Hull, C.W.: Apparatus for production of three-dimensional objects by stereolithography. U.S. Patent No. 45 753 301, 1986.Google Scholar
Ligon, S.C., Liska, R., Stampfl, J., Gurr, M., and Mulhaupt, R.: Polymers for 3D printing and customized additive manufacturing. Chem. Rev. 117, 10212 (2017).CrossRefGoogle ScholarPubMed
Chua, C.K. and Yeong, W.Y.: Bioprinting: Principles and Applications, Vol. 1 (World Scientific Publishing Co. Inc., Singapore, 2014).Google Scholar
Sean, V.M. and Anthony, A.: 3D bioprinting of tissues and organs. Nat. Biotechnol. 32, 773 (2014).Google Scholar
Tibbits, S.: The emergence of “4D printing”. In TED Conference, 2013.Google Scholar
Wan, Z., Zhang, P., Liu, Y., Lv, L., and Zhou, Y.: Four-dimensional bioprinting: current developments and applications in bone tissue engineering. Acta Biomater. 101, 26–42 (2019).CrossRefGoogle ScholarPubMed
Wang, C., Zhou, Y., and Wang, M.: In situ delivery of rhBMP-2 in surface porous shape memory scaffolds developed through cryogenic 3D plotting. Mater. Lett. 189, 140 (2017).CrossRefGoogle Scholar
Miao, S., Zhu, W., Castro, N.J., Nowicki, M., Zhou, X., Cui, H., Fisher, J.P., and Zhang, L.G.: 4D printing smart biomedical scaffolds with novel soybean oil epoxidized acrylate. Sci. Rep. 6, 27226 (2016).CrossRefGoogle ScholarPubMed
Zarek, M., Mansour, N., Shapira, S., and Cohn, D.: 4D printing of shape memory-based personalized endoluminal medical devices. Macromol. Rapid Commun. 38, 1600628 (2017).CrossRefGoogle ScholarPubMed
Zhang, L., Xiang, Y., Zhang, H., Cheng, L., Mao, X., An, N., Zhang, L., Zhou, J., Deng, L., and Zhang, Y.: A biomimetic 3D-self-forming approach for microvascular scaffolds. Adv. Sci. 1903553 (2020).CrossRefGoogle ScholarPubMed
Koons, G.L. and Mikos, A.G.: Progress in three-dimensional printing with growth factors. J. Control. Release 295, 50–59 (2019).CrossRefGoogle ScholarPubMed
Narayanan, L.K., Huebner, P., Fisher, M.B., Spang, J.T., Starly, B., and Shirwaiker, R.A.: 3D-bioprinting of polylactic acid (PLA) nanofiber–alginate hydrogel bioink containing human adipose-derived stem cells. ACS Biomater. Sci. Eng. 2, 1732 (2016).CrossRefGoogle Scholar
Zhou, Y., Zhao, Q., and Wang, M.: Dual release of VEGF and PDGF from emulsion electrospun bilayer scaffolds consisting of orthogonally aligned nanofibers for gastrointestinal tract regeneration. MRS Commun. 9, 1098 (2019).CrossRefGoogle Scholar
Poldervaart, M.T., Gremmels, H., van Deventer, K., Fledderus, J.O., Öner, F.C., Verhaar, M.C., Dhert, W.J., and Alblas, J.: Prolonged presence of VEGF promotes vascularization in 3D bioprinted scaffolds with defined architecture. J. Control. Release 184, 58 (2014).CrossRefGoogle ScholarPubMed
Bao, M., Lou, X., Zhou, Q., Dong, W., Yuan, H., and Zhang, Y.: Electrospun biomimetic fibrous scaffold from shape memory polymer of PDLLA-co-TMC for bone tissue engineering. ACS Appl. Mater. Interfaces 6, 2611–2621 (2014).CrossRefGoogle ScholarPubMed
Yue, K., Trujillo-de Santiago, G., Alvarez, M.M., Tamayol, A., Annabi, N., and Khademhosseini, A.: Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels. Biomaterials 73, 254–271 (2015).10.1016/j.biomaterials.2015.08.045CrossRefGoogle ScholarPubMed
Du, X., Cui, H., Sun, B., Wang, J., Zhao, Q., Xia, K., Wu, T., and Humayun, M.S.: Photothermally triggered shape-adaptable 3D flexible electronics. Adv. Mater. Technol. 2, 1700120 (2017).CrossRefGoogle Scholar
Wang, W., Li, C., Cho, M., and Ahn, S.H.: Soft tendril-inspired grippers: shape morphing of programmable polymer–paper bilayer composites. ACS Appl. Mater. Interfaces 10, 10419–10427 (2018).CrossRefGoogle ScholarPubMed
Van Den Bulcke, A.I., Bogdanov, B., De Rooze, N., Schacht, E.H., Cornelissen, M., and Berghmans, H.: Structural and rheological properties of methacrylamide modified gelatin hydrogels. Biomacromolecules 1, 31 (2000).CrossRefGoogle ScholarPubMed
Struik, L.: Orientation effects and cooling stresses in amorphous polymers. Polym. Eng. Sci. 18, 799–811 (1978).CrossRefGoogle Scholar
Li, H., Tan, Y.J., Liu, S., and Li, L.: Three-dimensional bioprinting of oppositely charged hydrogels with super strong interface bonding. ACS Appl. Mater. Interfaces 10, 11164 (2018).CrossRefGoogle ScholarPubMed
Lee, K.Y. and Mooney, D.J.: Alginate: properties and biomedical applications. Prog. Polym. Sci. 37, 106 (2012).CrossRefGoogle ScholarPubMed
Park, J.Y., Shim, J.H., Choi, S.A., Jang, J., Kim, M., Lee, S.H., and Cho, D.W.: 3D printing technology to control BMP-2 and VEGF delivery spatially and temporally to promote large-volume bone regeneration. J. Mater. Chem. B 3, 5415 (2015).CrossRefGoogle ScholarPubMed
Zhu, W., Cui, H., Boualam, B., Masood, F., Flynn, E., Rao, R.D., Zhang, Z.Y., and Zhang, L.G.: 3D bioprinting mesenchymal stem cell-laden construct with core–shell nanospheres for cartilage tissue engineering. Nanotechnology 29, 185101 (2018).CrossRefGoogle ScholarPubMed
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