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RNA Delivery via DNA-Inspired Janus Base Nanotubes for Extracellular Matrix Penetration

Published online by Cambridge University Press:  24 January 2020

Ian Sands ,
Jinhyung Lee ,
Wuxia Zhang  and
Yupeng Chen
Ian Sands
Affiliation:
Department of Engineering, University of Connecticut, Storrs, CT.
Jinhyung Lee
Affiliation:
Department of Engineering, University of Connecticut, Storrs, CT.
Wuxia Zhang
Affiliation:
Department of Engineering, University of Connecticut, Storrs, CT.
Yupeng Chen*
Affiliation:
Department of Engineering, University of Connecticut, Storrs, CT.
*
*Yupeng.chen@uconn.edu
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Abstract

RNA delivery into deep tissues with dense extracellular matrix (ECM) has been challenging. For example, cartilage is a major barrier for RNA and drug delivery due to its avascular structure, low cell density and strong negative surface charge. Cartilage ECM is comprised of collagens, proteoglycans, and various other noncollagneous proteins with a spacing of 20nm. Conventional nanoparticles are usually spherical with a diameter larger than 50-60nm (after cargo loading). Therefore, they presented limited success for RNA delivery into cartilage. Here, we developed Janus base nanotubes (JBNTs, self-assembled nanotubes inspired from DNA base pairs) to assemble with small RNAs to form nano-rod delivery vehicles (termed as “Nanopieces”). Nanopieces have a diameter of ∼20nm (smallest delivery vehicles after cargo loading) and a length of ∼100nm. They present a novel breakthrough in ECM penetration due to the reduced size and adjustable characteristics to encourage ECM and intracellular penetration.

Keywords

nanoscaleself-assemblybiomimetic (assembly)biomedical
Type
Articles
Information
MRS Advances , Volume 5 , Issue 16: Soft Materials and Biomaterials , 2020 , pp. 815 - 823
Copyright
Copyright © Materials Research Society 2020

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