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Magnetophoretic Behavior of 3T3 Cells Incubated with Saccharide-Coated MNPs

Published online by Cambridge University Press:  23 December 2016

Thomas W. Fallows ,
Thomas P. Coxon ,
Julie E. Gough  and
Simon J. Webb
Thomas W. Fallows*
Affiliation:
School of Chemistry and Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK School of Materials, University of Manchester, MSS Tower, Manchester, M13 9PL, UK
Thomas P. Coxon
Affiliation:
School of Chemistry and Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK School of Materials, University of Manchester, MSS Tower, Manchester, M13 9PL, UK
Julie E. Gough
Affiliation:
School of Materials, University of Manchester, MSS Tower, Manchester, M13 9PL, UK
Simon J. Webb
Affiliation:
School of Chemistry and Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK
*
*(Email: thomas.fallows@manchester.ac.uk)
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Abstract

Providing magnetite nanoparticles with saccharide coatings has been found to significantly increase the interactions of the nanoparticles with cells. Glucose (Glc) or N-acetylglucosamine (GlcNAc) coated magnetic nanoparticles (MNPs) were used to magnetically label 3T3 fibroblast cells, and the response of the labelled cells to external magnetic fields was studied. It was found that cells incubated with Glc- or GlcNAc-coated nanoparticles were much more likely to move towards an external magnet than those incubated with uncoated nanoparticles. Furthermore, cells in suspension moved much faster than those in contact with the surface of polystyrene well plates, with stronger magnets increasing the speed of movement. Cells that were adhering to the floor of the cell culture well and did not move in the x-y plane could still be rotated about the z-axis by moving the external magnet around the cell.

Keywords

biomaterialmagnetic propertiesnanoscale
Type
Articles
Information
MRS Advances , Volume 2 , Issue 24: Biomaterials and Soft Materials , 2017 , pp. 1279 - 1284
Copyright
Copyright © Materials Research Society 2016 

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Footnotes

†

These authors contributed equally to this work.

References

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