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Evaluation of species-specificity in barnacle waterborne settlement pheromones

Published online by Cambridge University Press:  27 November 2024

Shiori Kitade ,
Kiyotaka Matsumura  and
Yorisue Takefumi Open the ORCID record for Yorisue Takefumi [Opens in a new window]
Shiori Kitade
Affiliation:
Graduate School of Human Science and Environment, University of Hyogo, Himeji, Japan
Kiyotaka Matsumura
Affiliation:
School of Marine Science, Kitasato University, Sagamihara-Minami, Kanagawa, Japan
Yorisue Takefumi*
Affiliation:
Graduate School of Human Science and Environment, University of Hyogo, Himeji, Japan Institute of Natural and Environmental Sciences, University of Hyogo, Sanda, Hyogo, Japan Museum of Nature and Human Activities, Hyogo, Sanda, Hyogo, Japan
*
Corresponding author: Takefumi Yorisue; Email: yorisue@gmail.com
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Abstract

Larval settlement is an important process that drives population and community dynamics of marine invertebrates. Barnacles are frequently used to investigate settlement mechanisms of marine invertebrate larvae. Adult barnacles induce settlement of conspecific larvae nearby which ultimately facilitates copulation with neighbouring individuals. A significant factor involved in the larval induction process is the proteinaceous waterborne settlement pheromone (WSP), which is purified from adult barnacles. A previous study suggested that the concentration of WSP informs barnacle cyprid larvae about the abundance of adult barnacles in the environment nearby but it is unclear whether WSP works in a species-specific or non-species-specific manner. In this study, we conducted settlement assays using recombinant WSPs and cyprids of two congenic barnacle species, Amphibalanus amphitrite and A. improvisus, to investigate the species specificity of WSPs. We found that A. amphitrite and A. improvisus cyprids responded similarly to con-specific WSPs as to allo-specific WSP stimuli indicating that WSPs are not species-specific. Our findings suggest that cyprids approach potential settlement sites using WSPs, non-species-specific settlement pheromones, before performing a closer search of the substratum using a species-specific pheromone.

Keywords

barnaclecypridlarvasettlementspecies specificitywaterborne pheromone
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 104 , 2024 , e106
Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press on behalf of Marine Biological Association of the United Kingdom

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