Hostname: page-component-745bb68f8f-kw2vx Total loading time: 0 Render date: 2025-01-11T02:03:32.320Z Has data issue: false hasContentIssue false
  • English
  • Français

Duration and Timing of Spermatogenesis in a Stock of the Mussel Mytilus Californianus

Published online by Cambridge University Press:  11 May 2009

R. Norman Kelley ,
Michael J. Smith-Ashwood  and
Derek V. Ellis
R. Norman Kelley
Affiliation:
Biology Department, University of Victoria, Victoria, B.C., Canada, V8W 2Y2
Michael J. Smith-Ashwood
Affiliation:
Biology Department, University of Victoria, Victoria, B.C., Canada, V8W 2Y2
Derek V. Ellis
Affiliation:
Biology Department, University of Victoria, Victoria, B.C., Canada, V8W 2Y2
Get access Rights & Permissions [Opens in a new window]

Extract

Determining the duration and timing of spermatogenesis in Mytilus californianus Conrad became key questions in our study of carcinogen-mutagen indices in the marine environment. We had postulated that the murine sperm deformation assay of Bruce, Furrer & Wyrobek (1974) should have marine analogues, and we assayed a number of marine invertebrates sampled near potentially carcinogenic waste flows, and by γ-ray dose-response experiments. Our field results demonstrated some deformation but only at a very low level (less than 5 % in Mytilus edulis L.). Development of a γ-ray doseresponse (90–900 rads) procedure for M. californianus by radium needle inserted through a drilled hole closed with a plastic plug, and subsequent biopsy of gonadal tissue at measured distances from the site of irradiation, did not show deformations from a pilot test in June 1979. If a viable dose-response procedure was to be developed it was necessary to establish seasonal reproductive timing so that specimens could be collected or cultured to maximize spermatogenesis and hence response potential, and also to establish the duration of spermatogenesis so that response measures via a gonadal tissue extraction could be appropriately timed.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 62 , Issue 3 , August 1982 , pp. 509 - 519
Copyright
Copyright © Marine Biological Association of the United Kingdom 1982

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bartlett, B. R., 1972. Reproductive Ecology of the California Sea Mussel Mytilus californianus Conrad. M.Sc. Thesis, University of the Pacific, Stockton, California.Google Scholar
Beeman, R. D., 1970. An autoradiographic and phase contrast study of spermatogenesis in the anaspidean opisthobranch Phyllaplysia taylori Dall, 1900. Archives de zoologie expérimentale et generate, 111, 5–22.Google Scholar
Bruce, W. R., Furrer, R. & Wyrobek, A. J., 1974. Abnormalities in the shape of murine sperm after acute testicular X-irradiation. Mutation Research, 23, 381–386.CrossRefGoogle ScholarPubMed
Chipperfield, P. N. J., 1953. Observations on the breeding and settlement of Mytilus edulis (L.) in British waters. Journal of the Marine Biological Association of the United Kingdom, 32, 449–476.CrossRefGoogle Scholar
Elvin, D. W., 1975. Oogenesis in Mytilus californianus. Ph.D. Thesis, Oregon State University, Corvallis, Oregon.Google Scholar
Field, I. A., 1922. Biology and economic value of the sea mussel Mytilus edulis. Bulletin of the Bureau of Fisheries, 38, 127–260.Google Scholar
Franzen, A., 1955. Comparative morphological investigations into the spermiogenesis among mollusca. Zoologiska bidrag frdn Uppsala, 30, 399–458.Google Scholar
Galigher, A. E. & Kozloff, E. N., 1971. Essentials of Practical Microtechnique. 531 pp. Philadelphia: Lea & Febiger.Google Scholar
Grimstone, A. V. & Skaer, R. J., 1972. A Guidebook to Microscopical Methods. 134 pp. Cambridge University Press.Google Scholar
Holland, N. D. & Giese, A. C., 1965. An autoradiographic investigation of the gonads of the purple sea urchin (Strongylocentrotus purpuratus). Biological Bulletin. Marine Biological Laboratory, Woods Hole, Mass., 128, 241–258.CrossRefGoogle Scholar
Jessee, W. N., 1976. The Effects of Water Temperature, Tidal Cycles, and Intenidal Position on Spawning in Mytilus californianus (Conrad). M.A. Thesis, Humboldt State College, Arcata, California.Google Scholar
Longo, F. J. & Dornfeld, E. J., 1967. The fine structure of spermatid differentiation in the mussel, Mytilus edulis. Journal of Ultrastructure Research, 20, 462–480.CrossRefGoogle ScholarPubMed
Lubet, P., 1957. Cycle sexuel de Mytilus edulis L. et de Mytilus galloprovincialis Lmk. dans le Bassin d'Arcachon (Gironde). Annie biologique, 33, 19–29.Google Scholar
Lubet, P., 1959. Recherches sur le cycle sexuel et remission des gametes chez les Mytilidés et les Pectinidés (mollusques bivalves). Revue des travaux de l'Office (scientifique et technique) des peches maritimes, 23, 387–548.Google Scholar
Monesi, V., 1962. Autoradiographic study of DNA synthesis and the cell cycle in spermatogonia and spermatocytes of mouse testis using tritiated thymidine. Journal of Cell Biology, 14, 1–18.CrossRefGoogle ScholarPubMed
Mortreuil-Langlois, M., 1962. Staining sections coated with radiographic emulsion; a nuclear fast red, indigo-carmine sequence. Stain Technology, 37, 175–177.CrossRefGoogle ScholarPubMed
Niijima, L. & Dan, J., 1965. The acrosome reaction of Mytilus edulis. I. Fine structure of the intact acrosome. Journal of Cell Biology, 25, 243–248.CrossRefGoogle ScholarPubMed
Quayle, D. B., 1978. A preliminary report on the possibilities of mussel culture in British Columbia. Technical Report. Fisheries and Marine Service, no. 815, 37 pp.Google Scholar
Seed, R., 1969. The ecology of Mytilus edulis L. (Lamellibranchiata) on exposed rocky shores. I. Breeding and settlement. Oecologia, 3, 277–316.CrossRefGoogle ScholarPubMed
Seed, R., 1975. Reproduction in Mytilus (Mollusca: Bivalvia) in European waters. Pubblicazioni della Stazione zoologica di Napoli, suppl. 39, 317–334.Google Scholar
Seed, R., 1976. Ecology. In Marine Mussels: Their Ecology and Physiology (ed. Bayne, B. L.), pp. 13–65. Cambridge University Press. [International Biological Programme 10.]Google Scholar
Seed, R. & Brown, R. A., 1977. A comparison of the reproductive cycles of Modiolus modiolus (L.), Cerastoderma (=Cardium) edule (L.), and Mytilus edulis L. in Strangford Lough, Northern Ireland. Oecologia, 30, 173–188.CrossRefGoogle ScholarPubMed
Wilson, B. R. & Hodgkin, E. P., 1967. A comparative account of the reproductive cycles of five species of marine mussels (Bivalvia: Mytilidae) in the vicinity of Fremantle, Western Australia. Australian Journal of Marine and Freshwater Research, 18, 175–203.CrossRefGoogle Scholar
Wilson, J. H. & Seed, R., 1974. Reproduction in Mytilus edulis L. (Mollusca: Bivalvia) in Carlingford Lough, Northern Ireland. Irish Fisheries Investigations (B), no. 15, 30 pp.Google Scholar