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Evaluation of three-dimensional silver-doped borate bioactive glass scaffolds for bone repair: Biodegradability, biocompatibility, and antibacterial activity

Published online by Cambridge University Press:  25 August 2015

Hui Wang ,
Shichang Zhao ,
Xu Cui ,
Yangyi Pan ,
Wenhai Huang ,
Song Ye ,
Shihua Luo ,
Mohamed N. Rahaman ,
Changqing Zhang  and
Deping Wang
Hui Wang
Affiliation:
Department of Materials Science and Engineering, Institute of Bioengineering and Information Technology Materials, Tongji University, Shanghai 200092, China
Shichang Zhao
Affiliation:
Department of Orthopedic Surgery, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China
Xu Cui
Affiliation:
School of Materials Science and Engineering, Tongji University, Shanghai 200092, China
Yangyi Pan
Affiliation:
School of Materials Science and Engineering, Tongji University, Shanghai 200092, China
Wenhai Huang
Affiliation:
School of Materials Science and Engineering, Tongji University, Shanghai 200092, China
Song Ye
Affiliation:
School of Materials Science and Engineering, Tongji University, Shanghai 200092, China
Shihua Luo
Affiliation:
Department of Orthopaedic Surgery, Shanghai Jiaotong University Affiliated Ruijing Hospital, Shanghai 200233, China
Mohamed N. Rahaman
Affiliation:
Department of Materials Science and Engineering, and Center for Biomedical Science and Engineering, Missouri University of Science and Technology, Rolla, Missouri 65409, USA
Changqing Zhang*
Affiliation:
Department of Orthopedic Surgery, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China
Deping Wang*
Affiliation:
School of Materials Science and Engineering, Tongji University, Shanghai 200092, China
*
b)e-mail: shzhangchangqing@163.com
a)Address all correspondence to these authors. e-mail: wdpshk@tongji.edu.cn
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Abstract

The development of synthetic scaffolds with a desirable combination of properties, such as bioactivity, the ability to locally deliver antibacterial agents and high osteogenic capacity, is a challenging but promising approach in bone tissue engineering. In this study, scaffolds of a borosilicate bioactive glass (composition: 6Na2O, 8K2O, 8MgO, 22CaO, 36B2O3, 18SiO2, 2P2O5; mol%) with controllable antibacterial activity were developed by doping the parent glass with varying amounts of Ag2O (0.05, 0.5, and 1.0 wt%). The addition of the Ag2O lowered the compressive strength and degradation of the bioactive glass scaffolds but it did not affect the formation of hydroxyapatite on the surface of the glass as determined by energy dispersive x-ray analysis, x-ray diffraction, and Fourier transform infrared analysis. The Ag2O-doped scaffolds showed a sustained release of Ag ions over more than 8 weeks in simulated body fluid and resistance against colonization by the bacterial strains Escherichia coli and Staphylococcus aureus. In vitro cell culture showed better adhesion, proliferation, and alkaline phosphatase activity of murine osteoblastic MC3T3-E1 cells on the Ag2O-doped bioactive glass scaffolds than on the undoped scaffolds. The results indicate that these Ag-doped borosilicate bioactive glass scaffolds may have potential in repairing bone coupled with providing a lower risk of bacterial infection.

Keywords

borosilicate bioactive glassscaffoldssilver-doped bioactive glassbioactive glass conversionantibacterial activitybiocompatibility
Type
Articles
Information
Journal of Materials Research , Volume 30 , Issue 18 , 28 September 2015 , pp. 2722 - 2735
Copyright
Copyright © Materials Research Society 2015 

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