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3D printing of poly(ε-caprolactone)/poly(D,L-lactide-co-glycolide)/hydroxyapatite composite constructs for bone tissue engineering

Published online by Cambridge University Press:  27 June 2018

Kazim K. Moncal ,
Dong N. Heo ,
Kevin P. Godzik ,
Donna M. Sosnoski ,
Oliver D. Mrowczynski ,
Elias Rizk ,
Veli Ozbolat ,
Scott M. Tucker ,
Ethan M. Gerhard  and
Madhuri Dey
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Kazim K. Moncal
Affiliation:
Engineering Science and Mechanics, Pennsylvania State University, University Park, Pennsylvania 16802, USA; and Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, Pennsylvania 16802, USA
Dong N. Heo
Affiliation:
Engineering Science and Mechanics, Pennsylvania State University, University Park, Pennsylvania 16802, USA; and Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, Pennsylvania 16802, USA
Kevin P. Godzik
Affiliation:
Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, Pennsylvania 16802, USA; and Biomedical Engineering, Pennsylvania State University, University Park, Pennsylvania 16802, USA
Donna M. Sosnoski
Affiliation:
Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, Pennsylvania 16802, USA; and Department of Biology, Pennsylvania State University, University Park, Pennsylvania 16802, USA
Oliver D. Mrowczynski
Affiliation:
Department of Neurosurgery, College of Medicine, Pennsylvania State University, Hershey, Pennsylvania 17033, USA
Elias Rizk
Affiliation:
Department of Neurosurgery, College of Medicine, Pennsylvania State University, Hershey, Pennsylvania 17033, USA
Veli Ozbolat
Affiliation:
Engineering Science and Mechanics, Pennsylvania State University, University Park, Pennsylvania 16802, USA; Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, Pennsylvania 16802, USA; and Department of Mechanical Engineering, Ceyhan Engineering Faculty, Cukurova University, Adana 01330, Turkey
Scott M. Tucker
Affiliation:
Engineering Science and Mechanics, Pennsylvania State University, University Park, Pennsylvania 16802, USA; and Department of Orthopaedics and Rehabilitation, College of Medicine, Pennsylvania State University, Hershey 17033, Pennsylvania, USA
Ethan M. Gerhard
Affiliation:
Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, Pennsylvania 16802, USA; and Biomedical Engineering, Pennsylvania State University, University Park, Pennsylvania 16802, USA
Madhuri Dey
Affiliation:
Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, Pennsylvania 16802, USA; and Department of Chemistry, Pennsylvania State University, University Park, Pennsylvania 16802, USA
Gregory S. Lewis
Affiliation:
Engineering Science and Mechanics, Pennsylvania State University, University Park, Pennsylvania 16802, USA; and Department of Orthopaedics and Rehabilitation, College of Medicine, Pennsylvania State University, Hershey 17033, Pennsylvania, USA
Jian Yang
Affiliation:
Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, Pennsylvania 16802, USA; and Biomedical Engineering, Pennsylvania State University, University Park, Pennsylvania 16802, USA
Ibrahim T. Ozbolat*
Affiliation:
Engineering Science and Mechanics, Pennsylvania State University, University Park, Pennsylvania 16802, USA; Huck Institutes of the Life Sciences, Pennsylvania State University, University Park 16802, Pennsylvania, USA; Biomedical Engineering, Pennsylvania State University, University Park 16802, Pennsylvania, USA; and Materials Research Institute, Pennsylvania State University, University Park 16802, Pennsylvania, USA
*
a)Address all correspondence to this author. e-mail: ito1@psu.edu
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Abstract

Three-dimensional (3D) printing technology is a promising method for bone tissue engineering applications. For enhanced bone regeneration, it is important to have printable ink materials with appealing properties such as construct interconnectivity, mechanical strength, controlled degradation rates, and the presence of bioactive materials. In this respect, we develop a composite ink composed of polycaprolactone (PCL), poly(D,L-lactide-co-glycolide) (PLGA), and hydroxyapatite particles (HAps) and 3D print it into porous constructs. In vitro study revealed that composite constructs had higher mechanical properties, surface roughness, quicker degradation profile, and cellular behaviors compared to PCL counterparts. Furthermore, in vivo results showed that 3D-printed composite constructs had a positive influence on bone regeneration due to the presence of newly formed mineralized bone tissue and blood vessel formation. Therefore, 3D printable ink made of PCL/PLGA/HAp can be a highly useful material for 3D printing of bone tissue constructs.

Keywords

3D printingcomposite inkbone tissue regenerationcalvarial defects
Type
Invited Article
Information
Journal of Materials Research , Volume 33 , Issue 14: Focus Issue: 3D Printing of Biomaterials , 27 July 2018 , pp. 1972 - 1986
Copyright
Copyright © Materials Research Society 2018 

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