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Transcriptomics of Cruznema velatum (Nematoda: Rhabditidae) with a redescription of the species

Published online by Cambridge University Press:  20 July 2023

F. Guo Open the ORCID record for F. Guo [Opens in a new window] ,
D. Slos ,
H. Du ,
K. Li ,
H. Li Open the ORCID record for H. Li [Opens in a new window]  and
X. Qing Open the ORCID record for X. Qing [Opens in a new window]
F. Guo
Affiliation:
Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China
D. Slos
Affiliation:
Plant Sciences Unit, Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Merelbeke 9820, Belgium
H. Du
Affiliation:
Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China
K. Li
Affiliation:
College of Agriculture, Xinjiang Agricultural University, Urumqi 830052, China
H. Li*
Affiliation:
Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China College of Agriculture, Xinjiang Agricultural University, Urumqi 830052, China
X. Qing
Affiliation:
Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China
*
Corresponding author: Hongmei Li; Email: lihm@njau.edu.cn
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Abstract

Cruznema velatum isolated from soil in a chestnut orchard located at Guangdong province, China, is redescribed with morphology, molecular barcoding sequences, and transcriptome data. The morphological comparison for C. velatum and six other valid species is provided. Phylogeny analysis suggests genus Cruznema is monophyletic. The species is amphimix, can be cultured with Escherichia coli in 7–9 days from egg to egg-laying adult, and has a lifespan of 11 to 14 days at 20°C. The transcription data generated 45,366 unigenes; 29.9%, 31.3%, 24.8%, and 18.6% of unigenes were annotated in KOG, SwissProt, GO, and KEGG, respectively. Further gene function analysis demonstrated that C. velatum share the same riboflavin, lipoic acid, and vitamin B6 metabolic pathways with Caenorhabditis elegans and Pristionchus pacificus.

Keywords

life cyclemolecularmorphologyphylogenyredescriptiontranscriptome
Type
Research Paper
Information
Journal of Helminthology , Volume 97 , 2023 , e57
Copyright
© Nanjing Agricultural University, 2023. Published by Cambridge University Press

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