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The response of hamsters to primary and secondary infection with Trichinella spiralis and to vaccination with parasite antigens

Published online by Cambridge University Press:  05 June 2009

J.M. Behnke ,
M.S. Dehlawi ,
R. Rose ,
P.N. Spyropoulos  and
D. Wakelin
J.M. Behnke
Affiliation:
Experimental Parasitology Research Group, Department of Life Science, University of Nottingham, University Park, Nottingham NG7 2RD, UK
M.S. Dehlawi
Affiliation:
Experimental Parasitology Research Group, Department of Life Science, University of Nottingham, University Park, Nottingham NG7 2RD, UK
R. Rose
Affiliation:
Experimental Parasitology Research Group, Department of Life Science, University of Nottingham, University Park, Nottingham NG7 2RD, UK
P.N. Spyropoulos
Affiliation:
Experimental Parasitology Research Group, Department of Life Science, University of Nottingham, University Park, Nottingham NG7 2RD, UK
D. Wakelin
Affiliation:
Experimental Parasitology Research Group, Department of Life Science, University of Nottingham, University Park, Nottingham NG7 2RD, UK
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Abstract

The duration of primary infections with T. spiralis was dose-dependent with greater proportional loss of worms from heavily infected hamsters and longer persistence of worms in syngeneic DSN hamsters carrying initially low intensity infections. Intestinal worms were lost more rapidly from challenged immunized animals with over 80% loss of established worms by day 6 post infection, but survival of residual worms for a further 2 weeks. Hamsters carrying initially more than 140 intestinal worms began to lose weight during the second week indicating severe pathology at this stage of infection. Mucosal mast cell numbers increased from 50 cells/20 villus crypt units in uninfected animals to a peak in excess of 150 during week 4 pi, although intestinal mastocytosis persisted long after the loss of the majority of adult worms. Serum antibody responses to muscle stage larval antigen were detected in week 3 and increased subsequently. Both mastocytosis and antibody responses were more intense on secondary exposure to infection. Hamsters vaccinated with muscle stage larval antigen showed only a moderately accelerated loss of the intestinal phase but the fecundity of worms was severely suppressed. Overall it was concluded that the hamster host provided a model of trichinellosis that, in many respects was closer than mice and rats to the pattern of infection seen in economically and clinically important host species.

Type
Research Papers
Information
Journal of Helminthology , Volume 68 , Issue 4 , December 1994 , pp. 287 - 294
Copyright
Copyright © Cambridge University Press 1994

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