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The recovery of entomopathogenic nematodes from selected areas within Peninsular Malaysia

Published online by Cambridge University Press:  05 June 2009

J.M. Mason ,
A.R. Razak  and
D.J. Wright
J.M. Mason
Affiliation:
Department of Biology, Imperial College of Science, Technology and Medicine, Silwood Park, Ascot, Berks, SL5 7PY, UK
A.R. Razak
Affiliation:
Department of Plant Protection, Universiti Pertanian Malaysia, 43400 UPM Serdang, Selangor, Malaysia
D.J. Wright
Affiliation:
Department of Biology, Imperial College of Science, Technology and Medicine, Silwood Park, Ascot, Berks, SL5 7PY, UK
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Abstract

Soil sampling was conducted within Peninsular Malaysia with the aim of recovering entomopathogenic nematodes (Steinernematidae and Heterorhabditidae). Extensive sampling was performed in the Cameron Highlands, which are climatically distinct from the lowlands, and characterized by lower temperature and humidity. The major areas sampled in the lowlands were at the campus of Universiti Pertanian Malaysia (orchards and plantations), Puchong (secondary rainforest) and along the east coast of the country. Entomopathogenic nematodes were recovered using the Galleria mellonella baiting method. Nematodes were recovered from 10% of the 425 samples assayed. Identifications, using a PCR method, revealed that the 21 identified steinernematids belonged to two different genetic types and that four out of the five heterorhabditids were Heterorhabditis indicus, the remaining heterorhabditid being a new species. The nematodes are currently being screened to evaluate their biocontrol potential for use in Malaysia against foliage-feeding lepidopteran pests of crucifers.

Type
Research Papers
Information
Journal of Helminthology , Volume 70 , Issue 4 , December 1996 , pp. 303 - 307
Copyright
Copyright © Cambridge University Press 1996

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