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Recognition of deglycosylated larval proteins of Gnathostoma spinigerum by a monoclonal antibody and human gnathostomiasis antiserum

Published online by Cambridge University Press:  11 April 2024

P. Uparanukraw ,
T. Harnnoi ,
A. Dantrakool ,
N. Morakote  and
W. Chaicumpa
P. Uparanukraw*
Affiliation:
Department of Parasitology Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand
T. Harnnoi
Affiliation:
Department of Parasitology Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand
A. Dantrakool
Affiliation:
Department of Parasitology Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand
N. Morakote
Affiliation:
Department of Parasitology Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand
W. Chaicumpa
Affiliation:
Department of Microbiology and Immunology Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand
*
*Fax: 6653 217144 E-mail: puparanu@sd01.med.cmu.ac.th
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Abstract

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The study on the recognition of 35S-labelled somatic antigens of Gnathostoma spinigerum advanced third-stage larva (aL3) has revealed that the mAb GN6/24 immunoprecipitated 26- and 24-kDa proteins from the undigested and N-glycosidase F-digested larval extracts, respectively. The recognition of the deglycosylated form of the glycoprotein indicated that the mAb reacted with the peptide epitope on the 26-kDa protein. Human gnathostomiasis antiserum immunoprecipitated most of the N-glycosidase F-digested larval proteins including the deglycosylated 26-kDa protein.

Type
Other
Information
Journal of Helminthology , Volume 73 , Issue 3 , March 1999 , pp. 273 - 276
Copyright
Copyright © Cambridge University Press 1999

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