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Published online by Cambridge University Press: 05 June 2009
Paramphistomum explanation, Gastrothylax crumenifer, and Fasciola gigantica were maintained for periods of twelve hours in a non-nutrient medium containing streptomycin and penicillin to prevent bacterial growth. They were kept under aerobic conditions at a suitable temperature of 37%C. The protein content in P. explanatum ranged from 50·0% to 54·7% with an average of 52·96%±0·23 of the dry weight of the tissue. In G. crumenifer it ranged from 43·5% to 51·5% with an average of 48·8%±0·56 of d.w.t. In F. gigantica it ranged from 63·8% to 68·3% with an average of 66·5%±0·26 of d.w.t. The nitrogen content of the P. explanation excreted as ammonia after a starvation period of twelve hours was 3·95% and as uric acid it was 0·077%. In G. crumenifer the ammonia was 2·91% and uric acid 0·033%. In F. gigantica ammonia was 2·51% and uric acid 0·1%.