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Phylogenetic relationships and further unknown diversity of diplostomids (Diplostomida: Diplostomidae) parasitic in kingfishers

Published online by Cambridge University Press:  13 January 2023

T.J. Achatz ,
Z.S. Von Holten ,
J.W. Kipp ,
A. Fecchio Open the ORCID record for A. Fecchio [Opens in a new window] ,
L.R. LaFond ,
S.E. Greiman ,
J.R. Martens  and
V.V. Tkach Open the ORCID record for V.V. Tkach [Opens in a new window]
T.J. Achatz
Affiliation:
Department of Natural Sciences, Middle Georgia State University, Macon, Georgia, USA
Z.S. Von Holten
Affiliation:
Department of Natural Sciences, Middle Georgia State University, Macon, Georgia, USA
J.W. Kipp
Affiliation:
Department of Biology, University of North Dakota, Grand Forks, North Dakota, USA
A. Fecchio
Affiliation:
Centro de Investigación Esquel de Montaña y Estepa Patagónica (CIEMEP), CONICET – Universidad Nacional de la Patagonia San Juan Bosco, Esquel, Chubut, Argentina
L.R. LaFond
Affiliation:
Department of Biology, University of North Dakota, Grand Forks, North Dakota, USA
S.E. Greiman
Affiliation:
Department of Biology, Georgia Southern University, Statesboro, Georgia, USA
J.R. Martens
Affiliation:
Department of Biology, University of North Dakota, Grand Forks, North Dakota, USA
V.V. Tkach*
Affiliation:
Department of Biology, University of North Dakota, Grand Forks, North Dakota, USA
*
Author for correspondence: V.V. Tkach, E-mail: vasyl.tkach@und.edu
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Abstract

Kingfishers (Alcedinidae Rafinesque) are common inhabitants of wetlands and are known to be definitive hosts to a wide range of digeneans that parasitize fish as second intermediate hosts. Among these digeneans, members of the Diplostomidae Poirier, 1886 (diplostomids) are particularly common. Recent studies of diplostomids collected from kingfishers have revealed that they are probably more diverse than currently known. This particularly concerns the genera Crassiphiala Van Haitsma, 1925 and Uvulifer Yamaguti, 1934. In the present work, we studied seven diplostomid taxa from kingfishers in Brazil, the USA and the Philippines. Partial DNA sequences of the nuclear large ribosomal subunit (28S) and mitochondrial cytochrome c oxidase I (cox1) genes were obtained, and 28S sequences were used to study the phylogenetic interrelationships of these diplostomids. We provide the first DNA sequences from Uvulifer semicircumcisus Dubois et Rausch, 1950 and a member of Subuvulifer Dubois, 1952. Pseudocrassiphiala n. gen. is erected for a previously recognized species-level lineage of Crassiphiala and a new generic diagnosis of Crassiphiala is provided. Crassiphiala jeffreybelli n. sp., Crassiphiala wecksteini n. sp. and Pseudocrassiphiala tulipifera n. sp. are described, and a description of newly collected, high-quality specimens of Crassiphiala bulboglossa Van Haitsma, 1925 (the type-species of the genus) is provided.

Keywords

DiplostomidaeCrassiphialaPseudocrassiphiala n. genSubuvuliferUvuliferCrassiphiala bulboglossakingfishersmolecular phylogeny
Type
Research Paper
Information
Journal of Helminthology , Volume 97 , 2023 , e8
Copyright
Copyright © The Author(s), 2023. Published by Cambridge University Press

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