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Morphological and molecular phylogeny of Clinostomum sp. (Digenea: Clinostomidae) metacercariae, using DNA barcode from a South American freshwater fish

Published online by Cambridge University Press:  30 January 2025

T. Gião ,
M.I. Müller ,
F. Yamada ,
F. Freitas ,
L. Leite Open the ORCID record for L. Leite [Opens in a new window] ,
R.J. da Silva ,
R. de Azevedo  and
V. Abdallah
T. Gião
Affiliation:
Universidade Federal de Santa Catarina (UFSC), Florianópolis, Santa Catarina, Brasil
M.I. Müller
Affiliation:
Oregon State University (OSU), Department of Microbiology, Corvallis, Oregon, USA Universidade Estadual Paulista (UNESP), Instituto de Biociências, Departamento de Parasitologia, Botucatu, São Paulo, Brasil
F. Yamada
Affiliation:
Universidade Regional do Cariri (URCA), Crato, Ceará, Brasil
F. Freitas
Affiliation:
Centro Universitário Sagrado Coração (UNISAGRADO), Bauru, São Paulo, Brasil
L. Leite
Affiliation:
Universidade Federal de Alagoas (UFAL), Instituto de Ciências Biológicas e da Saúde, Departamento de Patologia e Parasitologia, Maceió, Alagoas, Brasil
R.J. da Silva
Affiliation:
Universidade Estadual Paulista (UNESP), Instituto de Biociências, Departamento de Parasitologia, Botucatu, São Paulo, Brasil
R. de Azevedo
Affiliation:
Centro universitário CESMAC, Maceió, Alagoas, Brasil
V. Abdallah*
Affiliation:
Universidade Federal de Alagoas (UFAL), Instituto de Ciências Biológicas e da Saúde, Departamento de Patologia e Parasitologia, Maceió, Alagoas, Brasil
*
Corresponding author: V. Abdallah; Email: vanessa.kozlowiski@icbs.ufal.br
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Abstract

Here, we present a comprehensive morphological and molecular phylogenetic analysis of Clinostomum sp. (Digenea: Clinostomidae) metacercariae parasitizing two freshwater fish species from Southeast Brazil: Serrasalmus spilopleura (piranha) and Callichthys callichthys (tambuatá). The morphological examination revealed distinct characteristics of metacercariae in each host. Using the cytochrome c oxidase I (COI) gene barcode region, we obtained DNA sequences that allowed for accurate phylogenetic placement. Phylogenetic analyses revealed that Clinostomum sp. HM41 (metacercariae), isolated from S. spilopleura, exhibited 86% similarity to Ithyoclinostomum yamagutii, while Clinostomum sp. HM125 (metacercariae), from C. callichthys, showed 98.7% similarity to Clinostomum sp. Cr_Ha1. The phylogenetic trees constructed through Bayesian Inference and Maximum Likelihood methods indicated high biodiversity within the Clinostomum genus and strong support for distinct lineages. These findings enhance our understanding of the diversity and ecological distribution of Clinostomum species in South American freshwater environments.

Keywords

Freshwater fishtrematodesClinostomum spmetacercariaeCOI
Type
Research Paper
Information
Journal of Helminthology , Volume 99 , 2025 , e13
Copyright
© The Author(s), 2025. Published by Cambridge University Press

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