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Epicutaneous sensitization with nematode antigens of fish parasites results in the production of specific IgG and IgE

Published online by Cambridge University Press:  07 August 2017

G. Fontenelle ,
M. Knoff ,
M.A. Verícimo  and
S.C. São Clemente
G. Fontenelle
Affiliation:
Pós-Graduação de Higiene Veterinária e Processamento Tecnológico de Produtos de Origem Animal, Faculdade de Medicina Veterinária, Universidade Federal Fluminense, Niterói, RJ, Brazil
M. Knoff*
Affiliation:
Laboratório de Helmintos Parasitos de Vertebrados, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil
M.A. Verícimo
Affiliation:
Departamento de Imunobiologia, Instituto de Biologia, Campus do Valonguinho, Universidade Federal Fluminense, Niterói, RJ, Brazil
S.C. São Clemente
Affiliation:
Laboratório de Inspeção e Tecnologia do Pescado, Departamento de Tecnologia de Alimentos, Faculdade de Medicina Veterinária, Universidade Federal Fluminense, Niterói, RJ, Brazil
*
*E-mail: knoffm@ioc.fiocruz.br
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Abstract

Fish consumption plays an important role in the human diet. Hoplias malabaricus, trahira, is a freshwater fish widely appreciated in several Brazilian states and it is frequently infected by Contracaecum multipapillatum third-instar larvae (L3). The aim of the present study was to evaluate the allergenic potential of the C. multipapillatum L3 crude extract (CECM). BALB/c mice were immunized intraperitoneally (ip) with 10 or 50 μg CECM associated with 2 mg of aluminium hydroxide on days 0, 14 and 48. The determination of specific IgG and IgE antibody levels was done after immunization, and the late immunity was evaluated by the intradermal reaction in the ear pavilion. Epicutaneous sensitization was performed in the dorsal region, with antigenic exposure via a Finn-type chamber, containing 100 μg of chicken ovum albumin (OVA) or 100 μg CECM. After the exposures, the specific antibody levels were determined. In the ip immunization, there was a gradual increase in IgG antibody levels, independent of CECM concentration. In relation to IgE production, it was transitory, and immunization with 10 μg was more efficient than that of 50 μg. The same result was observed in the cellular hypersensitivity reaction. In the case of antigen exposure by the epicutaneous route, it was verified that only CECM was able to induce detectable levels of specific IgG and IgE antibodies. In the present study it was demonstrated that both intraperitoneal immunization and epicutaneous contact with C. multipapillatum larval antigens are potentially capable of inducing allergic sensitization in mice.

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Type
Research Paper
Information
Journal of Helminthology , Volume 92 , Issue 4 , July 2018 , pp. 403 - 409
Copyright
Copyright © Cambridge University Press 2017 

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