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Alternative strategies for activation of the natural lactoperoxidase system in cows' milk: trials in Tanzania

Published online by Cambridge University Press:  09 July 2007

Leonard WT Fweja ,
Michael J Lewis  and
Alistair S Grandison
Leonard WT Fweja
Affiliation:
Department of Food Biosciences, The University of Reading, P O Box 226, Whiteknights, Reading, RG6 6AP, UK
Michael J Lewis
Affiliation:
Department of Food Biosciences, The University of Reading, P O Box 226, Whiteknights, Reading, RG6 6AP, UK
Alistair S Grandison*
Affiliation:
Department of Food Biosciences, The University of Reading, P O Box 226, Whiteknights, Reading, RG6 6AP, UK
*
*For correspondence; e-mail: a.s.grandison@reading.ac.uk
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Abstract

Thiocyanate content and lactoperoxidase activity of individual cow's milk of different breeds were determined, and the effects of different lactoperoxidase system (LP-s) activation strategies were compared. Lactoperoxidase activity varied significantly between Friesian and both Ayrshire and Tanzania Short Horn Zebu (TSHZ), but differences between Ayrshire and TSHZ were not significant. There was no significant variation in SCN− content between breeds. The LP-s was activated using three strategies based on SCN−: namely; equal concentrations of SCN− and H2O2 (7:7, 10:10, 15:15 mg/l), excess SCN− concentrations (15:10, 20:10, 25:10 mg SCN−:H2O2/l), and excess H2O2 concentrations (10:15, 10:20, 10:25 mg SCN−:H2O2/l), plus a fourth strategy based on I− (15:15 mg I−:H2O2/l). The keeping quality (KQ) was assessed using pH, titratable acidity, clot on boiling and alcohol stability tests. All activation strategies enhanced the shelf life of milk (typically increasing KQ from around 10 to around 20 h), but it was clear that the effectiveness of the LP-s depends on the type and concentrations of the activators of the system. The LP-s activated using I− as an electron donor was more effective than the LP-s activated using SCN− as an electron donor, increasing the KQ by a further 6–8 h compared with SCN−.

Keywords

Lactoperoxidasethiocyanateiodidehydrogen peroxidecow milkkeeping quality
Type
Research Article
Information
Journal of Dairy Research , Volume 74 , Issue 4 , November 2007 , pp. 381 - 386
Copyright
Copyright © Proprietors of Journal of Dairy Research 2007

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