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Crude glycerol as glycogenic precursor in feed; effects on milk coagulation properties and metabolic profiles of dairy cows

Published online by Cambridge University Press:  11 March 2013

Hedi Harzia ,
Kalle Kilk ,
Tiia Ariko ,
Marko Kass ,
Ursel Soomets ,
Ivi Jõudu ,
Tanel Kaart ,
David Arney ,
Olav Kärt  and
Meelis Ots
Hedi Harzia*
Affiliation:
Estonian University of Life Sciences, Institute of Veterinary Medicine and Animal Sciences, Kreutzwaldi 1, Tartu 51014, Estonia Bio-Competence Centre of Healthy Dairy Products, Kreutzwaldi 1, 51014 Tartu, Estonia
Kalle Kilk
Affiliation:
Department of Biochemistry, Medical Faculty, University of Tartu, the Centre of Excellence for Translational Medicine, 50411 Tartu, Estonia
Tiia Ariko
Affiliation:
Estonian University of Life Sciences, Institute of Veterinary Medicine and Animal Sciences, Kreutzwaldi 1, Tartu 51014, Estonia
Marko Kass
Affiliation:
Estonian University of Life Sciences, Institute of Veterinary Medicine and Animal Sciences, Kreutzwaldi 1, Tartu 51014, Estonia Bio-Competence Centre of Healthy Dairy Products, Kreutzwaldi 1, 51014 Tartu, Estonia
Ursel Soomets
Affiliation:
Department of Biochemistry, Medical Faculty, University of Tartu, the Centre of Excellence for Translational Medicine, 50411 Tartu, Estonia
Ivi Jõudu
Affiliation:
Estonian University of Life Sciences, Institute of Veterinary Medicine and Animal Sciences, Kreutzwaldi 1, Tartu 51014, Estonia Bio-Competence Centre of Healthy Dairy Products, Kreutzwaldi 1, 51014 Tartu, Estonia
Tanel Kaart
Affiliation:
Estonian University of Life Sciences, Institute of Veterinary Medicine and Animal Sciences, Kreutzwaldi 1, Tartu 51014, Estonia Bio-Competence Centre of Healthy Dairy Products, Kreutzwaldi 1, 51014 Tartu, Estonia
David Arney
Affiliation:
Estonian University of Life Sciences, Institute of Veterinary Medicine and Animal Sciences, Kreutzwaldi 1, Tartu 51014, Estonia
Olav Kärt
Affiliation:
Estonian University of Life Sciences, Institute of Veterinary Medicine and Animal Sciences, Kreutzwaldi 1, Tartu 51014, Estonia
Meelis Ots
Affiliation:
Estonian University of Life Sciences, Institute of Veterinary Medicine and Animal Sciences, Kreutzwaldi 1, Tartu 51014, Estonia Bio-Competence Centre of Healthy Dairy Products, Kreutzwaldi 1, 51014 Tartu, Estonia
*
*For correspondence; e-mail: hedi.harzia@emu.ee
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Abstract

As grain prices rise, the search for alternative glycogenic precursors in animal feed becomes increasingly important, and this study was conducted to determine if the replacement of starch with glycerol, as an alternative glycogenic precursor, affects the milk metabolic profile and milk coagulation ability, and therefore the quality of the milk. Eight primiparous mid-lactation Holstein cows were fed during a replicated 4 × 4 Latin square trial with four different isoenergetic rations: (1) control (T0) fed a total mixed ration (TMR) with barley meal; (2) group T1, decreased barley content, replaced isoenergetically with 1 kg crude glycerol; (3) group T2, the barley meal was replaced with 2 kg of crude glycerol; and (4) group T3 the barley meal was replaced with 3 kg of crude glycerol. Rumen, blood and milk samples were collected at the end of every 21-d treatment period. Rumen samples were analysed for proportion of total volatile fatty acid (VFA), blood samples for insulin and glucose, and milk for metabolites (e.g. citric-acid cycle compounds). The change in glycogenic precursors had a positive effect on rumen VFA proportions; the proportion of propionic acid increased (P < 0·001). Milk protein (P < 0·001) and curd firmness (P < 0·001) both increased. The increase in milk protein concentration may have been due to an increase in microbial protein. Regarding the milk metabolic profiles, different signals were positively associated with coagulation ability and change in the diet. Based on this study, changing the glycogenic precursor in animal diet in this way is possible, and may have no immediate deleterious consequences on milk quality or cow health. Indeed, there is evidence for benefits from this substitution.

Keywords

Glycerolglycogenic precursorsmilk coagulationmetabolomics

Information

Type
Research Article
Information
Journal of Dairy Research , Volume 80 , Issue 2 , May 2013 , pp. 190 - 196
Copyright
Copyright © Proprietors of Journal of Dairy Research 2013

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