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Some observations on the distribution and origin of nitrogen in sheep faeces

Published online by Cambridge University Press:  27 March 2009

V. C. Mason
V. C. Mason
Affiliation:
Department of Nutritional Biochemistry, Rowett Research Institute, Bucksburn, Aberdeen, AB2 9 SB
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Summary

1. Three methods, based on treatment with neutral detergent or acid detergent, or involving ultrasonic disintegration, are described and compared for the direct estimation of undigested dietary nitrogen in individual samples of sheep faeces. Estimates of the true digestibility of the nitrogen in several sheep diets derived from analyses performed with these methods agreed well with each other, and were in accord with published estimates, derived by extrapolation techniques. Two other methods, based on treatment with phenol–acetic acid–water, and lysozyme–trypsin, respectively, were found to be unsuitable for such estimates.

2. The quantitative distribution of nitrogen between undigested dietary residues, bacterial residues, endogenous debris residues and the water soluble fraction was determined chemically. It was concluded that 57–81% of the non-dietary faecal nitrogen was associated with bacterial material.

3. Indirect evidence suggested that most of the bacterial nitrogen in faeces originated in the rumen.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 73 , Issue 1 , August 1969 , pp. 99 - 111
Copyright
Copyright © Cambridge University Press 1969

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References

REFERENCES

Agricultural Research Council (1965). The nutrient requirements of farm livestock. No. 2. Ruminants. Technical Reviews and Summaries, pp. 157–8.Google Scholar
Becker, M. E. & Hartsell, S. E. (1955). The synergistic action of lysozyme and trypsin in bacteriolysis. Archs Biochem. Biophys. 55, 257–69.CrossRefGoogle ScholarPubMed
Bruce, J., Goodall, E. D., Kay, R. N. B., Phillipson, A. T. & Vowles, L. E. (1966). The flow of organic and inorganic materials through the alimentary tract of the sheep. Proc. R. Soc. B 166, 46–62.Google ScholarPubMed
Chibnall, A. C., Rees, M. W. & Williams, E. F. (1943). The total nitrogen of egg albumin and other proteins. Biochem. J. 37, 354–9.CrossRefGoogle ScholarPubMed
Dijkstra, N. D. (1966). Estimation of the nutritive value of fresh roughage. Proc. 10th int. Grassld Congr. (Helsinki), 393–7.Google Scholar
Drapala, W. J., Raymond, L. C. & Crampton, E. W. (1947). Pasture studies. XXVII. The effects of maturity of the plant and its lignification and subsequent digestibility by animals as indicated by methods of plant histology. Scient. Agric. 27, 36–41.Google Scholar
Elliott, R. C. & Fokkema, K. (1960). Protein digestibility relationships in ruminants. Rhodesia agric. J. 57, 301–4.Google Scholar
Elliott, R. C. & Topps, J. H. (1964). Studies on the protein requirement of ruminants. 3. Nitrogen balance trials on Blackhead Persian Sheep given diets of different energy and protein content. Br. J. Nutr. 18, 245–52.CrossRefGoogle ScholarPubMed
El-Shazly, K. & Hungate, R. E. (1966). Method of measuring Diaminopimelic acid in total rumen contents and its application to the estimation of bacterial growth. Appl. Microbiol. 14, 1, 27–30.CrossRefGoogle Scholar
Faichney, G. J. (1968). Volatile fatty acids in the caecum of the sheep. Aust. J. biol. Sci. 21, 177–80.CrossRefGoogle ScholarPubMed
Fujjiwara, T. & Akabori, S. (1954). α-ά Diaminopimelic acid from Chlorella ellipsoidea. J. chem. Soc. Japan (Pure Chemistry Section) 75, 990–3.Google Scholar
Gray, G. W. & Wilkinson, S. G. (1965). The effect of ethylenediaminetetra-acetic acid on the cell walls of some Gram-negative bacteria. J. gen. Microbiol. 39, 385–99.CrossRefGoogle Scholar
Greenhalgh, J. F. D. & Reid, G. W. (1967). Separating the effects of digestibility and palatability of food intake in ruminant animals. Nature, Lond. 214, 5089, p. 744.CrossRefGoogle Scholar
Grenet, E. (1966). Les particules végótales des fèces de mouton. Annls Zootech. 15, 303–12.CrossRefGoogle Scholar
Hellström, N. & Aamisepp, M. (1965). Digestion. III. Faecal analyses and digestibility. J. Sci. Fd. Agric. 16, 27–33.CrossRefGoogle Scholar
Hercus, B. H. (1960). Plant cuticle as an aid to determining the diet of grazing animals. Proc. 8th int. Grassld Cong. (Reading), 443–7.Google Scholar
Holter, J. A. & Reid, J. T. (1959). Relationship between concentration of crude protein and apparently digestible protein in forages. J. Anim. Sci. 18, 1339–49.CrossRefGoogle Scholar
Hughes, D. E. (1965). Biological effects of ultrasound. Sci. J. 1, 7, 39–45.Google Scholar
Hughes, D. E. & Nyborg, W. L. (1962). Cell disruption by ultrasound. Science, N.Y. 138, 108–14.CrossRefGoogle ScholarPubMed
Hungate, R. E. (1966). The Rumen and its Microbes. New York and London: Academic Press.Google Scholar
Jennings, A. C. & Watt, W. B. (1967). Fractionation of plant material. I. Extraction of proteins and nucleic acids from plant tissues and isolation of protein fractions containing hydroxyproline from broad bean (Vicia faba L) leaves. J. Sci. Fd Agric. 18, 527–35.CrossRefGoogle Scholar
Johnson, B. C., Hamilton, T. S., Robinson, W. B. & Garey, J. C. (1944). On the mechanism of nonprotein-nitrogen utilization by ruminants. J. Anim. Sci. 3, 287–98.CrossRefGoogle Scholar
Martin, D. J. (1964). Analysis of sheep diet utilizing plant epidermal fragments in faeces samples. In Grazing in Terrestrial and Marine Environments. Ed. Crisp, D. J.. London: Blackwell.Google Scholar
McNaught, M. L., Owen, E. C., Henry, K. M. & Kon, S. K. (1954). The utilization of non-protein nitrogen in the bovine rumen. 8. The nutritive value of the proteins of preparations of dried rumen bacteria, rumen protozoa and brewers yeast for rats. Biochem. J. 56, 151–6.CrossRefGoogle Scholar
Nasset, E. S. (1965). Role of the digestive system in protein metabolism. Fedn Proc. Fedn Am. Socs. exp. Biol. 24, 953–8.Google ScholarPubMed
ØRskov, E. R. & Fraser, C. (1968). Dietary factors influencing starch disappearance in various parts of the alimentary tract and caecal fermentation in oarly-weaned lambs. Proc. Nutr. Soc. 27, 37A–38A.Google Scholar
Pigden, W. J. & Hatina, G. (1966). Nitrogen metabolism in the ruminant. Res. Rep., 1965–6, Anim. Res. Inst., Ottawa, p. 13–14.Google Scholar
Putnam, F. W. (1948). The interactions of proteins and synthetic detergents. In Advances in Protein Chemistry, vol. IV, pp. 79–122. Ed. Anson, M. L. and Edsall, J. T.. New York: Academic Press Inc.Google Scholar
Reed, F. M., Moir, R. J. & Underwood, E. J. (1949). Rumen flora studies in the sheep. I. The nutritive value of rumen bacterial protein. Aust. J. sci. Res. Ser. B (2), 304–17.Google Scholar
Regal, V. (1960). The evaluation of the quality of pasture grasses by the microscope method. Proc. 8th int. Grassld Confr. (Reading), 522–4.Google Scholar
Repaske, R. (1956). Lysis of gram-negative bacteria by lysozyme. Biochim. biophys. Acta. 22, 189–91.CrossRefGoogle ScholarPubMed
Schneider, B. H. (1947). Feeds of the world. W. Virg. Agric. Expt. Stn., W. Virg.Google Scholar
Stolp, H. & Starr, M. P. (1965). Bacteriolysis. A. Rev. Microbiol. 19, 79–104.CrossRefGoogle ScholarPubMed
Synge, R. L. M. (1951). Non-protein nitrogenous constituents of ryegrass: Ionophoretic Fractionation and isolation of a ‘Bound Amino-Acid’ fraction. Biochem. J. 49, 642–50.CrossRefGoogle ScholarPubMed
Topps, J. H.Kay, R. N. B. & Goodall, E. D. (1968). Digestion of concentrate and hay diets in the stomach and intestines of ruminants. I. Sheep. Br. J. Nutr. 22, 261–80.CrossRefGoogle ScholarPubMed
Van Slyke, D. D., Dillon, R. T., MacFadyen, D. A. & Hamilton, P. (1941). Gasometric determination of carboxyl groups in free amino acids. J. biol. Chem. 141, 627–69.CrossRefGoogle Scholar
Van Soest, P. J. (1963). Use of detergents in the analysis of fibrous feeds. II. A rapid method for the determination of fibre and lignin. J. Ass. off. agric Chem. 46, 5, 928–35.Google Scholar
Van Soest, P. J. (1967). Development of a comprehensive system of feed analyses and its application to forages. J. Anim. Sci. 26, 1, 119–28.CrossRefGoogle Scholar
Van Soest, P. J. & Moore, L. A. (1965). New chemical methods for analysis of forages for the purpose of predicting nutritive value. Proc. 9th int. Grassld Congr. (Sao Paulo), 783–9.Google Scholar
Van Soest, P. J. & Wine, R. H. (1967). Use of detergents in the analysis of fibrous feeds. IV. Determination of plant cell wall constituents. J. Ass. off. agric. Chem. 50, 1, 50–5.Google Scholar
Virtanen, A. I. (1966). Milk production on a proteinfree feed, using urea and ammonium salts as the sole source of nitrogen. Proc. 10th int. Grassld Congr. (Helsinki), 19–29.Google Scholar
Weller, R. A., Gray, F. V. & Pilgrim, A. F. (1958). The conversion of plant nitrogen to microbial nitrogen in the rumen of the sheep. Br. J. Nutr. 12, 421–9.CrossRefGoogle ScholarPubMed
Work, E. (1957). Reaction of ninhydrin in acid solution with straight-chain amino acids containing two amino groups and its application to the estimation of α-ε-Diaminopimelic acid. Biochem. J. 67, 416–23.CrossRefGoogle Scholar
Work, E. & Dewey, D. L. (1953). The distribution of α-ε-Diaminopimelic acid among various micro-organisms. J. gen. Microbiol. 9, 394–406.CrossRefGoogle ScholarPubMed