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Surface area matters: An evaluation of swabs and surface area for environmental surface sampling of healthcare pathogens

Published online by Cambridge University Press:  13 June 2022

Rolieria M. West Open the ORCID record for Rolieria M. West [Opens in a new window] ,
Alicia M. Shams Open the ORCID record for Alicia M. Shams [Opens in a new window] ,
Monica Y. Chan Open the ORCID record for Monica Y. Chan [Opens in a new window] ,
Laura J. Rose Open the ORCID record for Laura J. Rose [Opens in a new window]  and
Judith A. Noble-Wang Open the ORCID record for Judith A. Noble-Wang [Opens in a new window]
Rolieria M. West*
Affiliation:
Clinical and Environmental Microbiology Branch, Division of Healthcare Quality and Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia
Alicia M. Shams
Affiliation:
Clinical and Environmental Microbiology Branch, Division of Healthcare Quality and Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia
Monica Y. Chan
Affiliation:
Clinical and Environmental Microbiology Branch, Division of Healthcare Quality and Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia
Laura J. Rose
Affiliation:
Clinical and Environmental Microbiology Branch, Division of Healthcare Quality and Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia
Judith A. Noble-Wang
Affiliation:
Clinical and Environmental Microbiology Branch, Division of Healthcare Quality and Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia
*
Author for correspondence: Rolieria M. West, E-mail: wgr2@cdc.gov
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Abstract

Flocked and foam swabs were used to sample five healthcare pathogens from three sizes of steel and plastic coupons; 26 cm2, 323 cm2, and 645 cm2. As surface area increased, 1–2 log10 decrease in recovered organisms (P < .05) was observed. Sampling 26-cm2 yielded the optimal median percent of pathogens recovered.

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Type
Concise Communication
Information
Infection Control & Hospital Epidemiology , Volume 44 , Issue 5 , May 2023 , pp. 834 - 836
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Creative Commons
This is a work of the US Government and is not subject to copyright protection within the United States. Published by Cambridge University Press on behalf of The Society for Healthcare Epidemiology of America.
Copyright
© Centers for Disease Control and Prevention, 2022

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References

Rawlinson, S, Ciric, L, Cloutman-Green, E. How to carry out microbiological sampling of healthcare environment surfaces? A review of current evidence. J Hosp Infect 2019;103:363–374.CrossRefGoogle ScholarPubMed
Hasan, JA, Japal, KM, Christensen, ER, Samalot-Freire, LC. In vitro production of Clostridium difficile spores for use in the efficacy evaluation of disinfectants: a precollaborative investigation. J AOAC Int 2011;94:259–272.10.1093/jaoac/94.1.259CrossRefGoogle ScholarPubMed
National Institutes for Occupational Safety and Health. Emergency response resources: surface sampling procedures for Bacillus anthracis spores from smooth, nonporous surfaces. Centers for Disease Control and Prevention website. https://www.cdc.gov/niosh/topics/emres/surfacesampling-bacillus-anthracis.html. Updated April 26, 2012. Accessed March 11, 2021.Google Scholar
Park, GW, Lee, D, Trefiletti, A, Hrsak, M, Shugart, J, Vinje, J. Evaluation of a new environmental sampling protocol for detection of human norovirus on inanimate surfaces. J Appl Environ Microbiol 2015;81:5987–5992.10.1128/AEM.01657-15CrossRefGoogle ScholarPubMed
Tufts, JA, Meyer, K, Calfee, M, Don Lee, S. Composite sampling of a Bacillus anthracis surrogate with cellulose sponge surface samplers from a nonporous surface. PLoS One 2014;9:e114082.CrossRefGoogle ScholarPubMed
Rose, L, Houston, H, Martinez-Smith, M, Lyons, A, Whitworth, C, Reddy, S, Noble-Wang, J. Factors influencing environmental sampling recovery of healthcare pathogens from nonporous surfaces with cellulose sponges. PLoS One 2022;17:e0261588.CrossRefGoogle ScholarPubMed
Katsikogianni, M. and Missirlis, YF. Concise review of mechanisms of bacterial adhesion to biomaterials and of techniques used in estimating bacteria–material interactions. Eur Cell Mater 2004;8:37–57.CrossRefGoogle ScholarPubMed
van Merode, AE, van der Mei, HC, Busscher, HJ, Krom, BP. Influence of culture heterogeneity in cell surface charge on adhesion and biofilm formation by Enterococcus faecalis. J Bacteriol 2006;188:2421–2426.10.1128/JB.188.7.2421-2426.2006CrossRefGoogle ScholarPubMed
West-Deadwyler, RM, Moulton-Meissner, HA, Rose, LJ, Noble-Wang, JA. Elution efficiency of healthcare pathogens from environmental sampling tools. Infect Control Hosp Epidemiol 2020;41:226–228.Google ScholarPubMed
McEldowney, S, Fletcher, M. The effect of temperature and relative humidity on the survival of bacteria attached to dry solid surfaces. Letts Appl Microbiol 1988;7:83–86.10.1111/j.1472-765X.1988.tb01258.xCrossRefGoogle Scholar
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