Hostname: page-component-7dd5485656-bvgqh Total loading time: 0 Render date: 2025-10-31T01:20:30.488Z Has data issue: false hasContentIssue false
  • English
  • Français

Weaning (acclimatization) of in vitro-produced banana plants

Published online by Cambridge University Press:  23 October 2009

John Charles Robinson  and
Victor Galán Sáuco
John Charles Robinson
Affiliation:
Du Roi Lab., P.O. Box 1147, Letsitele 0885, South Africa
Victor Galán Sáuco
Affiliation:
Inst. Canario Investig. Agrar. (ICIA), Apartado Correos 60, 38208 La Laguna, Tenerife, Canary Islands, Spain
Get access

Abstract

Introduction. The protocol describes a method for promoting the acclimatization and protection of rooted in vitro banana plants between rooting in the laboratory and their establishment in a nursery. The principle, key advantages, starting plant material and time required are presented. Materials and methods. This part describes the weaning house installation, the weaning medium used to transplant the vitroplants, the two steps for acclimatizing the plantlets: receipt of rooted laboratory plants in flasks and planting, and factors affecting the growth of the plantlets in weaning conditions (light, misting system, temperature, humidity, wind, pest control and nutrition). Possible problems for troubleshooting are listed. Results. Under normal conditions, new roots appear 4–5 days after the start of weaning, and new leaves start to grow within 8–10 days. At the end of the weaning phase, young plants are healthy, well developed and they can be established in a nursery.

Keywords

South AfricaSpainMusa sp.methodsvitroplantsweaningAfrique du SudEspagneMusa sp.méthodevitroplantsevrage

Information

Type
Research Article
Information
Fruits , Volume 64 , Issue 5 , September 2009 , pp. 325 - 332
Copyright
© CIRAD, EDP Sciences, 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

Daniells J.W., Smith M.K., Post-flask management of tissue-cultured bananas, Tech. Rep. 18, ACIAR, Canberra, Australia, 1991, 9 p.
Jaizme-Vega, M.C., Azcon, R., Responses of some tropical and subtropical cultures to endomycorrhizal fungi, Mycorrhiza 5 (1995) 213–217. CrossRef
Israeli Y., Lahav E., Reuveni O., In-vitro culture of bananas, in: Gowen S. (Ed.), Bananas and plantains, Chapman and Hall, London, UK, 1995, pp. 147–178.
Vuylsteke D.R., Talengera D., Post-flask management of micropropagated bananas and plantains, IITA, Ibadan, Nigeria, 1998, 15 p.
Arias, O., Valverde, M., Production y variación somaclonal de plantas de banano variedad Grande Naine producidas por cultivo de tejidos, Asbana (San Jose, Costa Rica) 28 (1987) 6–11.
Lahav E., Banana nutrition, in: Gowen S. (Ed.), Bananas and plantains, Chapman and Hall, London, UK, 1995, pp. 258–316.
Turner D.W., The response of the plant to the environment, in: Gowen S. (Ed.), Bananas and plantains, Chapman and Hall, London, UK, 1995, pp. 206–229.
Robinson J.C., Bananas and plantains, CAB Int., Wallingford, UK, 1996, 238 p.