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A Spot Test for Estimating Ureides in Soyabean Petioles

Published online by Cambridge University Press:  03 October 2008

Richard A. Glenister  and
Thomas A. LaRue
Richard A. Glenister
Affiliation:
Boyce Thompson Institute for Plant Research, Ithaca, New York 14853, USA
Thomas A. LaRue
Affiliation:
Boyce Thompson Institute for Plant Research, Ithaca, New York 14853, USA
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Summary

The ureides allantoic acid and allantoin form a bright pink compound with acidic p-dimethylamino cinnamaldehyde. It is possible to estimate the ureide content of a soyabean petiole segment by crushing it on filter paper and developing the colour with this reagent. There is a strong correlation between the spot test and estimates from colorimetric determination of ureides. In field grown soyabeans, there was a positive correlation between the petiole ureide content during pod fill and the integrated seasonal acetylene reduction activity. The spot test can be used to identify plants with low nitrogen fixation activity.

Information

Type
Research Article
Information
Experimental Agriculture , Volume 22 , Issue 4 , October 1986 , pp. 405 - 416
Copyright
Copyright © Cambridge University Press 1986

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References

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