Hostname: page-component-669899f699-7xsfk Total loading time: 0 Render date: 2025-04-26T11:41:34.178Z Has data issue: false hasContentIssue false
  • English
  • Français

CHANGES IN ANTIOXIDANT ISOZYMES AS A BIOMARKER FOR CHARACTERIZING HIGH TEMPERATURE STRESS TOLERANCE IN RICE (ORYZA SATIVA L.) SPIKELETS

Published online by Cambridge University Press:  12 December 2012

SMRUTI DAS ,
P. KRISHNAN ,
MONALISA NAYAK  and
B. RAMAKRISHNAN
SMRUTI DAS
Affiliation:
Division of Agricultural Physics, Indian Agricultural Research Institute, New Delhi 110012, India Division of Plant Physiology, Central Rice Research Institute, Cuttack 753 006, Orissa, India
P. KRISHNAN*
Affiliation:
Division of Agricultural Physics, Indian Agricultural Research Institute, New Delhi 110012, India Division of Plant Physiology, Central Rice Research Institute, Cuttack 753 006, Orissa, India
MONALISA NAYAK
Affiliation:
Division of Plant Physiology, Central Rice Research Institute, Cuttack 753 006, Orissa, India
B. RAMAKRISHNAN
Affiliation:
Division of Microbiology, Indian Agricultural Research Institute, New Delhi 110012, India
*
Corresponding author. Email: prameelakrishnan@yahoo.com
Get access Rights & Permissions [Opens in a new window]

Summary

High temperature stress at flowering can adversely affect rice yield, largely due to failure of fertilization. Oxidative damage can be a major reason inducing spikelet sterility in rice. In the present study, the effect of high temperatures on antioxidant metabolism in rice spikelets was characterised using nine different genotypes. Exposure to different temperatures at flowering stage revealed significant differences among various antioxidant enzymes in spikelets, both quantitatively and qualitatively. Spikelets of susceptible genotypes withstood temperature stress of up to 35 °C, those of moderately tolerant between 35 °C and 38 °C and those of tolerant genotypes up to 40 °C. Presence or absence, and changes in the isozyme intensities were consistent with alterations in their activities. Superoxide dismutase (SOD) isozymes II and III were present after exposure at 30 °C and 35 °C, while SOD I appeared above 40°C. Intensities of catalase isozymes I and III and the only isozyme of ascorbate peroxidase altered, while the only isozyme of guaical peroxidase and two (III and IV) of the four isozymes of catechol peroxidase disappeared after high temperature exposure of 45 °C. Thus, this work provides an evidence of the role of antioxidant metabolism in spikelets under high temperature stress conditions. Hence, changes in antioxidant isozymes in rice spikelets can be used as a biomarker for characterizing high temperature stress tolerance in rice spikelets.

Type
Research Article
Information
Experimental Agriculture , Volume 49 , Issue 1 , January 2013 , pp. 53 - 73
Copyright
Copyright © Cambridge University Press 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

REFERENCES

Cakmak, I. and Marschner, H. (1992). Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves. Plant Physiology 98:1222–1227.Google Scholar
Chowdhury, S. R. and Choudhury, M. A. (1985). Hydrogen peroxide metabolism as an index of water stress tolerance in Jute. Physiologia Plantarum 65:503–507.Google Scholar
Das, S., Nayak, M., Patra, B. C., Ramakrishnan, B. and Krishnan, P. (2010). Characterization of seeds of selected wild species of rice stored under high temperature and humidity conditions. Indian Journal of Biochemistry and Biophysics 47:178–184.Google Scholar
Djanaguiraman, M., Prasad, P. V. V. and Al-Khatib, K. (2011). Ethylene perception inhibitor 1-MCP decreases oxidative damage of leaves through enhanced antioxidant defense mechanisms in soybean plants grown under high temperature stress. Environmental and Experimental Botany 71:215–223.Google Scholar
Gomez, K. A. and Gomez, A. A. (1984). Statistical Procedures for Agricultural Research, 2nd edn. New York: John Wiley.Google Scholar
Hu, W., Hu, G. and Han, B. (2009). Genome-wide survey and expression profiling of heat shock proteins and heat shock factors revealed overlapped and stress specific response under abiotic stresses in rice. Plant Science 176:583–590.Google Scholar
Intergovernmental Panel on Climate Change (IPCC) (2007). Fourth Assessment Report of the Intergovernmental Panel on Climate Change: The Impacts, Adaptation and Vulnerability (Working Group III). New York, NY: Cambridge University Press.Google Scholar
Jagadish, S. V. K., Craufurd, P. Q. and Wheeler, T. R. (2007). High-temperature stress and spikelet fertility in rice (Oryza sativa L.). Journal of Experimental Botany 58:1627–1635.Google Scholar
Jagadish, S. V. K., Muthurajan, R., Oane, R., Wheelerm, T. R., Heuer, S., Bennett, J. and Craufurd, P. Q. (2010). Physiological and Proteomic approaches to address heat tolerance during anthesis in rice (Oryza sativa L.). Journal of Experimental Botany 61:143–156.Google Scholar
Krishnan, P., Ramakrishnan, B., Raja Reddy, K. and Reddy, V. R. (2011). High temperature stress effects on rice plant growth and yield. Advances in Agronomy (USA) 111:87–206.CrossRefGoogle Scholar
Krishnan, P. and Surya Rao, A. V. (2005). Effects of genotype and environment on seed yield and quality of rice. The Journal of Agricultural Science (Cambridge) 143:283–292.Google Scholar
Krishnan, P., Swain, D. K., Baskar, C., Nayak, S. K. and Dash, R. N. (2007). Simulation studies on the effects of elevated CO2 and temperature on rice yield in Eastern India. Agriculture, Ecosystem and Environment 122:233–242.CrossRefGoogle Scholar
Matsui, T. and Omasa, K. (2002). Rice (Oryza sativa L.) cultivars tolerant to high temperature at flowering: anther characteristics. Annals of Botany 89:683–687.CrossRefGoogle ScholarPubMed
Morita, S., Shiratsuchi, H., Takahashi, J. and Fujita, K. (2004). Effect of high temperature on grain ripening in rice plants. Analysis of the effects of high night and high day temperatures applied to the panicle and other parts of the plant. Japanese Journal of Crop Science 73:77–83.CrossRefGoogle Scholar
Nakano, Y. and Asada, K. (1981). Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant and Cell Physiology 22:867–880.Google Scholar
Oidaira, H., Sano, S., Koshiba, T. and Ushimaru, T. (2000). Enhancement of antioxidative enzyme activities in chilled rice seedlings. Journal of Plant Physiology 156:811–813.Google Scholar
Rao, M.V., Paliyath, G. and Ormrod, D. P. (1996). Ultraviolet-B and ozone-induced biochemical changes in antioxidant enzymes of Avabidopsis thaliana. Plant Physiology 110:125–136.Google Scholar
Satake, T. and Yoshida, S. (1978). High temperature induced sterility in indica rices at flowering. Japanese Journal of Crop Science 47:6–17.Google Scholar
Scandalios, J. G., Tsaftaris, A. S., Chandlee, J. M. and Skadsen, R. M. (1984). Expression of the developmentally regulated catalase (Cat) genes in maize. Developmental Genetics 4:281–293.CrossRefGoogle Scholar
Sheehy, J. E., Mitchell, P. L., Beerling, D. J., Tsukaguchi, T. and Woodward, F. I. (1998). Temperature of rice spikelets: thermal damage and the concept of a thermal burden. Agronomie 18:449–460.Google Scholar
Srivalli, B., Sharma, G. and Khanna-Chopra, R. (2003). Antioxidative defense system in an upland rice cultivar subjected to increasing intensity of water stress followed by recovery. Physiologia Plantarum 119:503–512.Google Scholar
Tanida, M. (1996). Catalase activity of rice seed embryo and its relation to germination rate at a low temperature. Breeding Science 46:23–27.Google Scholar
Türkan, Bor M., Zdemir, F. and Koca, H. (2005). Differential responses of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolius Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Sciences 168:223–231.Google Scholar
Wahid, A., Gelani, S., Ashraf, M. and Foolad, M. R. (2007). Heat tolerance in plants: an overview. Environmental and Experimental Botany 61:199–223.Google Scholar
Wassmann, R., Jagadish, S. V. K., Sumfleth, K., Pathak, H., Howell, G., Ismail, A., Serraj, R., Redon˜a, E., Singh, R. K. and Heuer, S. (2009). Regional vulnerability of climate change impacts on Asian rice production and scope for adaptation. Advances in Agronomy 102:91–133.Google Scholar
Yamakawa, H. and Hakata, M. (2010). Atlas of rice grain filling-related metabolism under high temperature: joint analysis of metabolome and transcriptome demonstrated inhibition of starch accumulation and induction of amino acid accumulation. Plant Cell Physiology 51:795–809.Google Scholar
Yoshida, S., Satake, T. and Mackill, D. J. (1981). High-Temperature Stress in Rice (Review), IRRI Research Paper Series, 67. Manila, Philippines: International Rice Research Institute, 1–15.Google Scholar
Zhao, X., Nishimura, Y., Fukumoto, Y. and Li, J. (2011). Effect of high temperature on active oxygen species, senescence and photosynthetic properties in cucumber leaves. Environmental and Experimental Botany 70:212–216.CrossRefGoogle Scholar