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β-Lactam resistance in normal faecal flora from South Africa

Published online by Cambridge University Press:  15 May 2009

P. M. A. Shanahan ,
C. J. Thomson  and
S. G. B. Amyes
P. M. A. Shanahan
Affiliation:
Department of Medical Microbiology, The Medical School, University of Edinburgh, Teriot Place, Edinburgh EH8 9AG, UK
C. J. Thomson
Affiliation:
Department of Medical Microbiology, The Medical School, University of Edinburgh, Teriot Place, Edinburgh EH8 9AG, UK
S. G. B. Amyes*
Affiliation:
Department of Medical Microbiology, The Medical School, University of Edinburgh, Teriot Place, Edinburgh EH8 9AG, UK
*
* Author for correspondence.
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The genetic and biochemical basis of ampicillin resistance amongst the aerobic Gram-negative commensal faecal flora of healthy volunteers in South Africa has been determined. Amongst 608 ampicillin resistant strains isolated from 320 of the participants, 158 were able to transfer their ampicillin resistant determinants into Escheriehia coli K-12 J62–2. Iso-electric focusing of the β-lactamases, extracted from the transconjugants, demonstrated that ampicillin resistance resulted from the presence of the TEM-1, TEM-2 and SHV-1 β-lactamases in 94·3%, 2·5% and 3·2% of isolates respectively. Endonuclease restriction digests of the plasmids isolated from the transconjugants showed that the β-lactamase genes were present on a wide variety of plasmid types; 101 distinct plasmid endonuclease restriction patterns were identified. Transferable ampicillin resistance was associated with resistance to other antibiotics at the following frequencies: trimethoprim (48·7 %), streptomycin (35·4 %), tetracycline (27·2%), spectinomycin (9·5%), chloramphenicol (3·2%) and gentamicin (1·3%). One antibiotic resistance pattern, ampicillin and trimethoprim. predominated (28%). In total. 77·9% of the plasmids conferred resistance to other antibiotics raising the possibility that use of any of these agents, not simply ampicillin, may contribute to the maintenance of resistance genes.

Type
Research Article
Information
Epidemiology & Infection , Volume 115 , Issue 2 , October 1995 , pp. 243 - 253
Copyright
Copyright © Cambridge University Press 1995

References

1.Levy, SB, Marshall, B, Schluederberg, S, Rowse, D, Davis, J. High frequency of antimicrobial resistance in human faecal flora. Antimicrob Agents Chemother 1988; 32: 1801–6.CrossRefGoogle Scholar
2.Bonten, M, Stobberingh, E, Philips, J, Houben, A. Antibiotic resistance of Escherichia coli in faecal samples of healthy people in two different areas in an industrialized country. Infection 1992; 20: 258–62.CrossRefGoogle Scholar
3.Lester, SC, del Pilar Pla, M, Wang, F, Perez, Schael I, Jiang, H, O'Brien, TF. The carriage of Escherichia coli resistant to antimicrobial agents by healthy children in Boston, in Caracas. Venezuela, and in Qin Pu, China. N Eng J Med 1990; 323: 285–9.CrossRefGoogle ScholarPubMed
4.Amyes, SGB, Tait, S, Thomson, CJ et al. , The incidence of antibiotic resistance in aerobi faecal flora in South India. J Antimicrob Chemother 1992; 9: 415–25.CrossRefGoogle Scholar
5.Shanahan, PMA, Wylie, BA, Adrian, PV, Koornhof, HJ, Thomson, CJ, Amyes, SGB. The prevalence of antimicrobial resistance in human faecal flora in South Africa. Epidemiol Infect 1993; 111: 221–8.CrossRefGoogle ScholarPubMed
6.Shanahan, PMA, Thomson, CJ, Amyes, SGB. β-lactam resistance in aerobic commensal faecal flora. Int J Antimicrob Agents 1994; 3: 259–66.CrossRefGoogle Scholar
7.Mamun, KZ, Shears, P, Hart, CA. The prevalence and genetics of resistance to commonly used antimicrobial agents in faecal Enterobacteriaceae from children in Bangladesh. Epidemiol Infect 1993; 110: 447–58.CrossRefGoogle ScholarPubMed
8.Amyes, SGB. The success of plasmid-encoded drug resistance genes in clinical bacteria: an examination of plasmid-mediated ampicillin and trimethoprim resistance genes and their resistance mechanisms. The 10th C. L. Oakley Lecture. J Med Microbiol 1989; 28: 73–83.CrossRefGoogle ScholarPubMed
9.Young, H, Nandivada, LS, Amyes, SGB. Antibiotic resistance in the tropics. 1. The genetics of bacterial ampicillin resistance in tropical areas. Trans R Soc Trop Med Hyg 1989; 83: 38–41.CrossRefGoogle ScholarPubMed
10.Medeiros, AA. β-lactamases. Br Med Bull 1984; 40: 18–27.CrossRefGoogle ScholarPubMed
11.Medeiros, AA, Mayer, KH, Opal, SM. Plasmid-mediated beta-lactamases. Antimicrob Newsletter 1988; 5: 61–5.CrossRefGoogle Scholar
12.Roy, C, Segura, C, Torrellas, A, Reig, R, Teruel, D, Hermida, M. Activity of amoxycillin/ clavulanate against β-lactamase producing Escherichia coli and Klebsiella spp. J Antimicrob Chemother 1989; 24 Suppl B; 41–7.CrossRefGoogle ScholarPubMed
13.Thomson, CJ, Shanahan, PMA, Amyes, SGB. TEM-1 plasmids in the community. Lancet 1994; 343: 921.CrossRefGoogle ScholarPubMed
14.Shears, P, Suliman, G, Hart, CA. Restriction endonuclease characterization of resistant plasmids in Enterobacteriaceae isolated from children in the Sudan. Epidemiol Infect 1989; 103: 487–96.CrossRefGoogle ScholarPubMed
15.Shears, P, Suliman, G, Hart, CA. Occurrence of multiple antibiotic resistance and R plasmids in Enterobacteriaceae isolated from children in the Sudan. Epidemiol Infect 1988; 100: 73–81.CrossRefGoogle Scholar
16.Farrar, WE. Antibiotic resistance in developing countries. J Infect Dis 1985; 152: 1103–6.CrossRefGoogle ScholarPubMed
17.Amyes, SGB, Gould, IM. Trimethoprim resistant plasmids in faecal bacteria. Ann Microbiol Inst Pasteur 1984; 135B: 177–86.CrossRefGoogle Scholar
18.Takahashi, S, Nagano, Y. Rapid procedure for isolation of plasmid DNA and application to epidemiological analysis. J Clin Microbiol 1984; 20: 608–13.CrossRefGoogle ScholarPubMed
19.Davis, BD, Mingioli, ES. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol 1950; 60: 17–28.CrossRefGoogle ScholarPubMed
20.Working Party of the British Society for Antimicrobial Chemotherapy. A guide to sensitivity testing. J Antimicrob Chemother; 27 Suppl D: 1–50.Google Scholar
21.Matthew, M, Harris, AM, Marshall, MJ, Ross, GW. The use of analytical iso-electric focusing for detection and identification of beta-lactamases. J Gen Microbiol 1975; 88: 169–78.CrossRefGoogle Scholar
22.Waddell, W.T. A simple ultraviolet spectrophotometric method for the determination of protein. J Lab Clin Med 1956; 48: 311–4.Google ScholarPubMed
23.Shanahan, PMA, Thomson, CJ, Amyes, SGB. The global impact of antibiotic-resistant bacteria: their sources and reservoirs. Rev Med Microbiol 1994; 5: 174–82.CrossRefGoogle Scholar
24.Reves, RR, Murray, BE, Pickering, LK, Prado, D, Maddock, M, Bartlett, AV. Children with trimethoprim- and ampicillin-resistant faecal Escherichia coli in day care centers. J Infect Dis 1987; 156: 758–62.CrossRefGoogle ScholarPubMed
25.Thomson, CJ, Tail, S, Jesudason, MV, Amyes, SGB. Dissemination of the TEM-1 beta-lactamase gene into disparate plasmids in faecal strains of Escherichia coli isolated in Vellore, South India. J Infect 1993; 27: 47–50.CrossRefGoogle ScholarPubMed
26.Thomson, CJ, Amyes, SGB. Molecular epidemiology of the plasmid-encoded TEM-1 β-lactamase in Scotland. Epidemiol Infect; 110: 117–25.CrossRefGoogle Scholar
27.Roy, C, Teruel, D, Reig, R, Hermida, M, Teixell, M. β-lactamases and susceptibility phenotypes to β-lactam antibiotics in Escherichia coli strains. J Antimicrob Chemother 1992; 29: 593–4.CrossRefGoogle ScholarPubMed
28.Sirot, DL, Goldstein, FW, Soussy, CJ et al. , Resistance to cefotaxime and 7 other beta-lactams in members of the family Enterobacteriaceae – a 3-year survey in France. Antimicrob Agents Chemother 1992; 36: 1677–81.CrossRefGoogle Scholar
29.Bauernfeind, A, Rosenthal, E, Eberlein, E, Holley, M, Schweighart, S. Spread of Klebsiella pneumoniae, producing SHV-5 beta-lactamase among hospitalized patients. Infection 1993; 21: 18–22.CrossRefGoogle ScholarPubMed
30.Cullmann, W. The threat of resistance to the new oral cephalosporins. Chemotherapy 1992; 38: 10–7.CrossRefGoogle Scholar
31.Wu, P-J, Shannon, K, Phillips, I. Effect of hyperproduction of TEM-1 β-lactamase on in vitro susceptibility of Escherichia coli to β-lactam antibiotics. Antimicrob Agents Chemother 1994; 38: 494–8.CrossRefGoogle ScholarPubMed