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In vivo transfer of R factors between Escherichia coli strains inoculated into the rumen of sheep

Published online by Cambridge University Press:  15 May 2009

M. G. Smith
M. G. Smith
Affiliation:
CSIRO Division of Food Research, P.O. Box 12, Cannon Hill, Queensland, Australia, 4170
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Summary

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Substantial transfer of R factors occurred in vivo, under certain conditions, in the rumen of adult sheep in the absence of any antibiotic treatment. A starvation period of 24–48 hr. was required to produce the conditions necessary, when even quite low inocula (ca. 103 cells) of donor and recipient E. coli could grow within the rumen and reach a population density sufficient for transfer to take place. The results indicate that under the same conditions R factors may be transferred between organisms in the lower intestinal tract also. Without the starvation period, the inoculation of even massive numbers (1010 cells) of the same organisms resulted in almost no detectable transfer.

Some of the experimental animals on which a starvation period was imposed became carriers of either the inoculated recipient E. coli, or of R factor bearing coliforms, and these formed 1–10% of the total coliform population of the faeces for at least 6 weeks.

Type
Research Article
Information
Journal of Hygiene , Volume 75 , Issue 3 , December 1975 , pp. 363 - 370
Copyright
Copyright © Cambridge University Press 1975

References

REFERENCES

Anderson, E. S. (1968). The ecology of transferable drug resistance in the enterobacteria. Annual Review of Microbiology 22, 131–80.CrossRefGoogle ScholarPubMed
Anderson, J. D. (1973). The effect of resistance-transfer (R) factor carriage upon the survival of Escherichia coli in vitro and experimentally in man. Journal of Medical Microbiology 6 (4), xix.Google ScholarPubMed
Babcock, G. F., Berryhill, D. L. & Marsh, D. H. (1973). R-Factors of Escherichia coli from dressed beef and humans. Applied Microbiology 25, 21–3.Google ScholarPubMed
Brownlie, L. E. & Grau, F. H. (1967). Effect of food intake on growth and survival of salmonellas and Escherichia coli in the bovine rumen. Journal of General Microbiology 46, 125–34.CrossRefGoogle ScholarPubMed
Clowes, R. C. (1973). The molecule of infectious drug resistance. Scientific American 228 (4), 18–27.CrossRefGoogle ScholarPubMed
Grau, F. H., Brownlie, L. E. & Smith, M. G. (1969). Effects of food intake on numbers of salmonellae and Escherichia coli in rumen and faeces of sheep. Journal of Applied Bacteriology 32, 112–17.CrossRefGoogle ScholarPubMed
Jarolmen, H. (1971). Experimental transfer of antibiotic resistance in swine. Annals of the New York Academy of Sciences 182, 72–9.CrossRefGoogle ScholarPubMed
Smith, H. Williams (1971). Observations on the in vivo transfer of R factors. Annals of the New York Academy of Sciences 182, 80–90.CrossRefGoogle Scholar
Walton, J. R. (1970). Contamination of meat carcasses by antibiotic-resistant coliform bacteria. Lancet ii, 561–3.CrossRefGoogle Scholar