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The development of TRIC organisms in cell cultures during multiple infection

Published online by Cambridge University Press:  15 May 2009

Andrea Evans
Andrea Evans
Affiliation:
Medical Research Council Trachoma Unit, Lister Institute of Preventive Medicine, London SW 1
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Summary

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The relationship between multiplicity of infection and the yield of organisms and of polysaccharide was studied in BHK 21 cells infected with TRIC organisms. Although an increase in themultiplicity of infection resulted in an increase in the absolute number of organisms per culture, there was a decrease in relation to the number of infecting organisms. The amount of polysaccharide produced was independent of multiplicity of infection; it was not limited by the concentration of glucose in the medium. Polysaccharide leaked from cells and was slowly broken down in the culture medium.

Information

Type
Research Article
Information
Journal of Hygiene , Volume 70 , Issue 1 , March 1972 , pp. 39 - 48
Copyright
Copyright © Cambridge University Press 1972

References

REFERENCES

Bernkopf, H., Mashiah, P. & Becker, Y. (1962). Correlation between morphological and biochemical changes and the appearance of infectivity in FL cell cultures infected with trachoma agent. Annals of theNew York Academy of Sciences 98, 62–81.CrossRefGoogle ScholarPubMed
Bernkopf, H., Treu, T. & Maythar, B. (1964). Human infection experiments with three cell-cultured trachoma agents. Archives of Ophthalmology 71, 693–700.CrossRefGoogle ScholarPubMed
Blyth, W. A. & Taverne, J. (1972). Some consequences of the multiple infection of cells by TRIC organisms. Journal of Hygiene 70, 33–7.CrossRefGoogle ScholarPubMed
Dulbecco, R. & Vogt, M. (1954). Plaque formation and isolation of pure lines with poliomyelitis virus. Journal of Experimental Medicine 99, 167–82.CrossRefGoogle Scholar
Furness, G. & Fraser, E. F. (1962). One-step growth curves for inclusion blennorrhoea virus in HeLa cell monolayers. Journal of General Microbiology 27, 299–304.CrossRefGoogle ScholarPubMed
Gear, J. H. S., Gordon, F. B., Jones, B. R. & Bell, S. D. Jr (1963). Nomenclature of isolates of virus from trachoma and inclusion blennorrhoea. Nature, London 197, 26.CrossRefGoogle Scholar
Gill, S. D'A. & Stewart, R. B. (1970). Glucose requirements of L cells infected with Chlamydia psittaci. Canadian Journal of Microbiology 16, 997–1001.CrossRefGoogle ScholarPubMed
Gordon, F. B., Dressler, H. R. & Quan, A. L. (1967). Relative sensitivity of cell culture and yolk sac for detection of TRIC infection. American Journal of Ophthalmology 63, 1044–8.CrossRefGoogle ScholarPubMed
Gordon, F. B. & Quan, A. L. (1965). Occurrence of glycogen in inclusions of the psittacosis-lymphogranuloma venereum-trachoma agents. Journal of Infectious Diseases 115, 186–96.CrossRefGoogle Scholar
Jenkin, H. M. (1966). The continuous passage of agents of trachoma in cell culture: I. Characteristics of TW-3 and Bour strains of trachoma in serial passage in HeLa 229 cells. Journal of Infectious Diseases 116, 390–99.CrossRefGoogle ScholarPubMed
Jenkin, H. M. & Fan, V. S. C. (1971). Contrast of glycogenesis of Chlamydia trachomatis and Chlamydia psittaci strains in HeLa cells. In Trachoma and Related Disorders. Proceedings of a symposium held in Boston, Mass. August 1970. Ed. Nichols, R. L., pp. 52–9. Amsterdam: Excerpta Medica.Google Scholar
Mokrasch, L. C. (1954). Analysis of hexose phosphates and sugar mixtures with the anthrone reagent. Journal of Biological Chemistry 208, 55–59.CrossRefGoogle ScholarPubMed
Moulder, J. W. (1964). The psittacosis group as bacteria. CIBA Lectures in Microbial Biochemistry. John Wiley and Sons, Inc.Google Scholar
Reeve, P. & Taverne, J. (1962). A simple method for total particle counts of trachoma and inclusion blennorrhoea viruses. Nature, London 195, 923–4.CrossRefGoogle ScholarPubMed
Reeve, P. & Taverne, J. (1964). Growth in the chick embryo of strains of trachoma and inclusion blennorrhoea virus of differing virulence. Journal of General Microbiology 37, 277–84.Google Scholar
Reeve, P. & Taverne, J. (1967). Strain differences in the behaviour of TRIC agents in cell cultures. American Journal of Ophthalmology 63, 1167–73.CrossRefGoogle Scholar
Stoker, M. & Macpherson, I. (1964). Syrian hamster fibroblast cells, line BHK-21 and its derivatives. Nature, London 203, 1355–7.CrossRefGoogle Scholar
T'Ang, F. F., Chang, H. L., Huang, Y. T. & Wang, K. C. (1957). Studies on the etiology of trachoma with special reference to isolation of the virus in chick embryo. Chinese Medical Journal 75, 429–47.Google Scholar
Taverne, J. & Blyth, W. A. (1971). Interactions between trachoma organisms and macrophages. In Trachoma and Related Disorders. Proceedings of a symposium held in Boston, Mass. August 1970. Ed. Nichols, R. L., pp. 88–107. Amsterdam: Excerpta Medica.Google Scholar
Vies, J. Van Der (1954). Two methods for the determination of glycogen in liver. Biochemical Journal 57, 410–16.CrossRefGoogle ScholarPubMed