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Comparison of procedures based upon Rappaport–Vassiliadis medium with those using Muller–Kauffmann medium containing Teepol for the isolation of Salmonella sp.

Published online by Cambridge University Press:  19 October 2009

P. Vassiliadis ,
Ch. Mavromati ,
D. Trichopoulus ,
V. Kalapothaki  and
J. Papadakis
P. Vassiliadis
Affiliation:
The Hellenic Pasteur Institute, Athens 11521, Greece
Ch. Mavromati
Affiliation:
The Hellenic Pasteur Institute, Athens 11521, Greece
D. Trichopoulus
Affiliation:
The Department of Hygiene and Epidemiology, University of Athens, Athens 11527, Greece
V. Kalapothaki
Affiliation:
The Department of Hygiene and Epidemiology, University of Athens, Athens 11527, Greece
J. Papadakis
Affiliation:
Athens School of Hygiene, Athens 11521, Greece
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A total of 308 samples of different types were examined for the presence of salmonellas by means of three different procedures. The first consisted of preenrichment in buffered peptone water followed by enrichment in Rappaport-Vassiliadis medium (P/RV). The second differed only in that 1% Teepol was added to the pre-enrichment medium (PT/RV). In the third, buffered peptone water with 1% Teepol was followed by enrichment in Muller–Kauffmann tetrathionate broth also containing 1% Teepol (PT/MKT). The first of these combinations (P/RV) proved superior to the others both in terms of isolation rates and in the appearance of suspicious colonies.

Information

Type
Research Article
Information
Epidemiology & Infection , Volume 99 , Issue 1 , August 1987 , pp. 143 - 147
Copyright
Copyright © Cambridge University Press 1987

References

REFERENCES

Anonymous (1975). International Standard ISO 3565. Meat and meat-products – Detection of Salmonella (reference method), 1st edn, 1975–09–01. Geneva: International Organisation for Standardisation.Google Scholar
Edel, W. & Kampelmacher, E. H. (1909). Salmonella isolation in nine European laboratories using a standardized technique. Bulletin of the World Health Organization 41, 297–300.Google Scholar
Edel, W. & Kampelmacher, E. H. (1973). Comparative studies on the isolation of sublethally injured Salmonella in nine European laboratories. Bulletin of the World Health Organization 48, 167–174.Google ScholarPubMed
Kalapothaki, V., Trichopoulos, D., Papadakis, J., Mavrommati, Ch. & Vassiliadis, P. (1986). A comparison of the efficiency of two forms of Rappaport-Vassiliadis enrichment medium. International Journal of Food Microbiology 3, 89–97.Google Scholar
Skovgaard, N., Christensen, S. G. & Gulistani, A. W. (1985). Salmonellas in Danish pigs: a comparison of three isolation methods. Journal of Hygiene 95, 69–75.CrossRefGoogle ScholarPubMed
Vassiliadis, P. (1983). The Rappaport–Vassiliadis (RV) enrichment medium for the isolation of salmonellas: An overview. Journal of Applied Bacteriology 54, 69–70.CrossRefGoogle ScholarPubMed
Vassiliadis, P., Pateraki, E., Papaiconomou, N., Papadakis, J. A. & Trichoroulos, D. (1976). Nouveau precédé d'enrichissement de Salmonella. Annales de Microbiologie (Institut Pasteur) 127B, 195–200.Google Scholar
Vassiliadis, P., Truchopoulos, D., Papadakis, J., Kalapothaki, V. & Serie, Ch. (1979). Brilliant green deoxycliolate agar as an improved selective medium for the isolation of Salmonella. Annales de la Socie'te Beige de Me'decine Tropicale 59, 117–1120.Google Scholar
Vassiliadis, P., Trichopoulos, D., Papadakis, J., Kalapothaki, V., Zavitsanos, X. & Serie, Ch. (1981 a). Salmonella isolation with Rappaport's enrichment medium of different compositions. Zentralblatt für Iiakteriologie Originate B Hygiene Prarentire Medizine 173, 382–389.Google Scholar
Vassiliadis, P., Kalapothaki, V., Trichopoulos, D., Mavrommati, Ch. & Serie, Ch. (1981 b). Improved isolation of salmonellae from naturally contaminated meat products by using Rappaport–Vassiliadis enrichment broth. Applied and Environmental Microbiology 42, 615–618.CrossRefGoogle ScholarPubMed