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Characterisation of haemolytic activity from Aeromonas caviae

Published online by Cambridge University Press:  15 May 2009

T. Karunakaran  and
B. G. Devi
T. Karunakaran*
Affiliation:
Madurai Kamaraj University, Madurai
B. G. Devi
Affiliation:
Kuvempu University, B. R. Project, India
*
*Corresponding author and current address: The University of Texas Health Sciences Center. San Antonio. TX 78284–7894, USA.
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Aeromonas caviae, an enteropathogen associated with gastroenteritis, displays several virulence characteristics. Studies on the kinetics of growth of A. caviae and expression of β–haemolytic toxin revealed that A. caviae produced maximum haemolytic activity extracellularly during the stationary phase. Preliminary studies on the properties of A. caviae haemolysin suggested that divalent cations (Mg2+ and Ca2+) and thiol compounds, dithiothreitol and mercaptoethanol enhanced the haemolytic activity. Addition of L–cysteine, glutathione and EDTA reduced the haemolytic activity. The iron chelator, 2–2' bipyridyl, significantly inhibited the growth of A. caviae possibly by iron limitation, with parallel enhancement of haemolysin production compared to A. caviae grown in excess of iron. These results suggest that A. caviae produces only β-haemolysin, which resembles the haemolysins reported for several other bacteria and the activity might be regulated by environmental factors especially iron.

Information

Type
Research Article
Information
Epidemiology & Infection , Volume 112 , Issue 2 , April 1994 , pp. 291 - 298
Copyright
Copyright © Cambridge University Press 1994

References

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