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DIGESTIVE PROCESSES OF HAEMATOPHAGOUS INSECTS: V. INHIBITORS OF TRYPSIN FROM GLOSSINA MORSITANS MORSITANS (DIPTERA: GLOSSINIDAE)

Published online by Cambridge University Press:  31 May 2012

R. H. Gooding
R. H. Gooding
Affiliation:
Department of Entomology, University of Alberta, Edmonton
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Abstract

Trypsin has been partially purified from the midguts of Glossina morsitans. It is inactivated by tosyl-l-lysine chloromethyl ketone, phenylmethane sulfonylfluoride, and 2-mercaptoethanol. Mammalian sera inhibit G. morsitans trypsin in vitro. Sheep serum contains at least two inhibitors of G. morsitans trypsin and a high molecular weight material which protects against serum inhibition. Very low levels of trypsin inhibitor are found in G. morsitans primary excreta and in the salivary glands, Malpighian tubules, and anterior midguts of unfed flies. High concentrations of trypsin inhibitor are found in the anterior (non-digestive) section of the midgut for at least 48 h after flies fed on goats.

Type
Articles
Information
The Canadian Entomologist , Volume 106 , Issue 1 , January 1974 , pp. 39 - 44
Copyright
Copyright © Entomological Society of Canada 1974

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