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The synergistic effect of Metarhizium anisopliae CQMa421 and deltamethrin improved the insecticidal ability against to Bactrocera dorsalis

Published online by Cambridge University Press:  03 July 2025

Jinyuan Zhao ,
Chen Shen ,
Yangjunlu Shen ,
Lijuan Liu ,
Wenkai Yan ,
Yujia Guan ,
Renwen Zheng ,
Sihan Lu  and
Qingfeng Tang Open the ORCID record for Qingfeng Tang [Opens in a new window]
Jinyuan Zhao
Affiliation:
School of Plant Protection, Anhui Province Key Laboratory of Integrated Pest Management on Crops, Anhui Agricultural University, Hefei, 230036 China
Chen Shen
Affiliation:
School of Plant Protection, Anhui Province Key Laboratory of Integrated Pest Management on Crops, Anhui Agricultural University, Hefei, 230036 China
Yangjunlu Shen
Affiliation:
School of Plant Protection, Anhui Province Key Laboratory of Integrated Pest Management on Crops, Anhui Agricultural University, Hefei, 230036 China
Lijuan Liu
Affiliation:
School of Plant Protection, Anhui Province Key Laboratory of Integrated Pest Management on Crops, Anhui Agricultural University, Hefei, 230036 China
Wenkai Yan
Affiliation:
School of Plant Protection, Anhui Province Key Laboratory of Integrated Pest Management on Crops, Anhui Agricultural University, Hefei, 230036 China
Yujia Guan
Affiliation:
School of Plant Protection, Anhui Province Key Laboratory of Integrated Pest Management on Crops, Anhui Agricultural University, Hefei, 230036 China
Renwen Zheng
Affiliation:
School of Plant Protection, Anhui Province Key Laboratory of Integrated Pest Management on Crops, Anhui Agricultural University, Hefei, 230036 China
Sihan Lu
Affiliation:
School of Plant Protection, Anhui Province Key Laboratory of Integrated Pest Management on Crops, Anhui Agricultural University, Hefei, 230036 China
Qingfeng Tang*
Affiliation:
School of Plant Protection, Anhui Province Key Laboratory of Integrated Pest Management on Crops, Anhui Agricultural University, Hefei, 230036 China
*
Corresponding author: Qingfeng Tang; Email: tangqf@ahau.edu.cn
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Abstract

Bactrocera dorsalis (Diptera: Tephritidae) is a highly invasive and destructive quarantine pests worldwide. To improved biological control efficiency, reduce chemical pesticides use, and optimise the application of Metarhizium anisopliae (Hypocreales: Clavicipitaceae) against B. dorsalis. This study evaluated the combined toxicity of M. anisopliae with deltamethrin and chlorpyrifos. The biocompatibility of M. anisopliae CQMa421 with these pesticides was assessed based on spore germination, mycelial growth, and sporulation. Additionally, the effects of combined treatments on detoxification enzyme and related gene expression in B. dorsalis were investigated. The results indicated that the virulence effect of M. anisopliae CQMa421 against B. dorsalis adults was time-dependent and dose-dependent. Deltamethrin showed good compatibility with M. anisopliae CQMa421, achieving 100% mortality at 1 × 10⁸ CFU/mL by 84 hours. Different concentrations of deltamethrin can promote the mycelial growth and sporulation of M. anisopliae CQMa421. The toxicity effect of deltamethrin and chlorpyrifos combined with M. anisopliae CQMa421 on B. dorsalis adults was better than that of single-agent treatment, and the co-toxicity factor of 5 mg/L deltamethrin and 1 × 108 CFU/mL M. anisopliae CQMa421 was 24.81, which synergistically affected on B. dorsalis control. Enzyme activity assays and qRT-PCR results revealed that the combination treatment differentially activated and enhanced the activities of AChE, CarE, GST, CAT, and SOD. Meanwhile, BdCarE was significantly inhibited and upregulating BdGSTD7, BdGSTS1, BdCYP4ae1, BdPOD, BdPOD1, and BdCAT genes. In conclusion, the combination of deltamethrin and M. anisopliae CQMa421 enhanced the insecticidal efficacy against B. dorsalis, significantly affected the activity of related detoxification enzymes. Provided a robust basis for integrating biological and chemical control strategies to manage B. dorsalis more effectively.

Keywords

Bactrocera dorsaliscombinationdeltamethrinMetarhizium anisopliaesynergistic effect

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Type
Research Paper
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Bulletin of Entomological Research , First View , pp. 1 - 9
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© The Author(s), 2025. Published by Cambridge University Press.

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These authors have contributed equally to this work

References

Balabanidou, V, Grigoraki, L and Vontas, J (2018) Insect cuticle: A critical determinant of insecticide resistance. Current Opinion in Insect Science 27, 68–74.10.1016/j.cois.2018.03.001CrossRefGoogle ScholarPubMed
Cao, Y, Yang, Q, Tu, XH, Li, SG and Liu, S (2018) Molecular characterization of a typical 2-cys thioredoxin peroxidase from the asiatic rice borer Chilo suppressalis and its role in oxidative stress. Archives of Insect Biochemistry and Physiology 99, e21476.10.1002/arch.21476CrossRefGoogle ScholarPubMed
Clarke, AR, Armstrong, KF, Carmichael, AE, Milne, JR, Raghu, S, Roderick, GK and Yeates, DK (2005) Invasive phytophagous pests arising through a recent tropical evolutionary radiation: The Bactrocera dorsalis complex of fruit flies. Annual Review of Entomology 50, 293–319.CrossRefGoogle ScholarPubMed
Clarke, AR, Li, ZH, Qin, YJ, Zhao, ZH, Liu, LJ and Schutzeet, MK (2019) Bactrocera dorsalis (Hendel)(Diptera: Tephritidae) is not invasive through Asia: It’s been there all along. Journal of Applied Entomology 143, 797–801.10.1111/jen.12649CrossRefGoogle Scholar
de Faria, MR and Wraight, SP (2007) Mycoinsecticides and Mycoacaricides: A comprehensive list with worldwide coverage and international classification of formulation types. Biological Control 43, 237–256.10.1016/j.biocontrol.2007.08.001CrossRefGoogle Scholar
Ekesi, S, De Meyer, M, Mohamed, SA, Virgilio, M and Borgemeister, C (2016) Taxonomy, ecology, and management of native and exotic fruit fly species in Africa. Annual Review of Entomology 61, 219–238.10.1146/annurev-ento-010715-023603CrossRefGoogle ScholarPubMed
Enayati, AA, Ranson, H and Hemingway, J (2005) Insect glutathione transferases and insecticide resistance. Insect Molecular Biology 14, 3–8.10.1111/j.1365-2583.2004.00529.xCrossRefGoogle ScholarPubMed
Evangelos, B and Elias, K (2021) Additive and synergistic interactions of entomopathogenic fungi with Bacillus thuringiensis for the control of the European grapevine moth Lobesia botrana (Denis and Schiffermüller)(Lepidoptera: Tortricidae). Egyptian Journal of Biological Pest Control 31, 109.Google Scholar
Fournier, D and Mutero, A (1994) Modification of acetylcholinesterase as a mechanism of resistance to insecticides. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 108, 19–31.Google Scholar
Gao, P, Tan, JJ, Peng, X, Song, Y, Qu, MJ and Chen, MH (2024) Expression pattern of RpCSP6 from phopalosiphum padi and its binding mechanism with deltamethrin: Insights into chemosensory protein-mediated insecticide resistance. Journal of Agricultural and Food Chemistry 72, 17847–17857.CrossRefGoogle ScholarPubMed
Ge, MY, Zhang, TT, Zhang, M and Cheng, LG (2021) Ran participates in deltamethrin stress through regulating the nuclear import of nrf2. Gene 769, 145213.10.1016/j.gene.2020.145213CrossRefGoogle ScholarPubMed
Gichuhi, J, Khamis, F, Berg, JVD, Mohamed, S and Herren, JK (2020) Influence of inoculated gut bacteria on the development of Bactrocera dorsalis and on its susceptibility to the entomopathogenic fungus, Metarhizium anisopliae. BMC Microbiology 20, 321.CrossRefGoogle Scholar
Hsu, JC, Feng, HT and Wu, WJ (2004) Resistance and synergistic effects of insecticides in Bactrocera dorsalis (Diptera: Tephritidae) in Taiwan. Journal of Economic Entomology 97, 1682–1688.CrossRefGoogle ScholarPubMed
Hsu, JC, Feng, HT, Wu, WJ, Geib, SM, Mao, CH and Vontaset, J (2012) Truncated transcripts of nicotinic acetylcholine subunit gene Bdα6 are associated with spinosad resistance in Bactrocera dorsalis. Journal of Economic Entomology 42, 806–815.Google ScholarPubMed
Jayanth, PDK, Kempraj, V, Aurade, RM and Bruce, TJA (2017) Evaluation of synthetic oviposition stimulants to enhance egg collection of the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae). Journal of Pest Science 90, 781–786.10.1007/s10340-017-0845-0CrossRefGoogle Scholar
Jin, T, Zeng, L, Lin, Y and Liang, GW (2011) Insecticide resistance of the oriental fruit fly, Bactrocera dorsalis (Hendel)(Diptera: Tephritidae), in mainland China. Pest Management Science 67, 370–376.CrossRefGoogle ScholarPubMed
Kumar, S, Molina-Cruz, A, Gupta, L, Rodrigues, J and Barillas-Mury, C (2010) A peroxidase/dual oxidase system modulates midgut epithelial immunity in Anopheles gambiae. Science 327, 1644–1648.10.1126/science.1184008CrossRefGoogle ScholarPubMed
Lacey, LA, Grzywacz, D, Shapiro-Ilan, DI, Frutos, R, Brownbridge, M and Goettel, MS (2015) Insect pathogens as biological control agents: Back to the future. Journal of Invertebrate Pathology 132, 1–141.CrossRefGoogle ScholarPubMed
Li, XZ, Wang, GL, Wang, CL, Li, WJ, Lu, T, Ge, YG, Xu, CK, Zhong, X, Wang, JG and Yang, HY (2024) Long-term monitoring of Bactrocera and Zeugodacus spp. (Diptera: Tephritidae) in China and evaluation of different control methods for Bactrocera dorsalis (Hendel). Crop Protection 182, 106708.10.1016/j.cropro.2024.106708CrossRefGoogle Scholar
Ling, SF and Zhang, H (2013) Influences of chlorpyrifos on antioxidant enzyme activities of Nilaparvata lugens. Ecotoxicology & Environmental Safety 98, 187–190.CrossRefGoogle ScholarPubMed
Mansour, NA, Eldefrawi, ME, Toppozada, A and Zeid, M (1966) Toxicological studies on the egyptian cotton leaf worm, Prodenia litura. vi. potentiation and antagonism of organophosphorus and carbamate insecticides. Journal of Economic Entomology 59, 307–311.10.1093/jee/59.2.307CrossRefGoogle Scholar
Martin, T, Chandre, F, Ochou, OG, Vaissayre, M and Fournier, D (2002) Pyrethroid resistance mechanisms in the cotton bollworm Helicoverpa armigera (Lepidoptera: Noctuidae) from West Africa. Pesticide Biochemistry and Physiology 74, 17–26.10.1016/S0048-3575(02)00117-7CrossRefGoogle Scholar
Nauen, R, Bass, C, Feyereisen, R and Vontas, J (2022) The Role of Cytochrome P450s in insect toxicology and resistance. Annual Review of Entomology 67, 105–124.10.1146/annurev-ento-070621-061328CrossRefGoogle ScholarPubMed
Paula, AR, Carolino, AT, Paula, CO and Samuels, RI (2011) The combination of the entomopathogenic fungus Metarhizium anisopliae with the insecticide Imidacloprid increases virulence against the dengue vector Aedes aegypti (Diptera: Culicidae). Parasites and Vectors 4, 8.10.1186/1756-3305-4-8CrossRefGoogle ScholarPubMed
Pedrini, N, Crespo, R and Juárez, MP (2007) Biochemistry of insect epicuticle degradation by entomopathogenic fungi. Comparative Biochemistry and Physiology C: Toxicology & Pharmacology 146, 124–137.Google ScholarPubMed
Quintela, ED, Mascarin, GM, da Silva, RA, Barrigossi, JAF and Martins, JFD (2013) Enhanced susceptibility of Tibraca limbativentris (Heteroptera: Pentatomidae) to Metarhizium anisopliae with sublethal doses of chemical insecticides. Biological Control 66, 56–64.10.1016/j.biocontrol.2013.03.018CrossRefGoogle Scholar
Satoh, T and Hosokawa, M (1998) The mammalian carboxylesterases: From molecules to functions. Annual Review of Pharmacology and Toxicology 38, 257–288.10.1146/annurev.pharmtox.38.1.257CrossRefGoogle ScholarPubMed
Sheehan, D, Meade, G, Foley, VM and Dowd, CA (2001) Structure, function and evolution of glutathione transferases: Implications for classification of non-mammalian members of an ancient enzyme superfamily. Biochemical Journal 360, 1–16.CrossRefGoogle ScholarPubMed
Vargas, RI, Piñero, JC and Leblanc, L (2015) An overview of pest species of Bactrocera fruit flies (Diptera: Tephritidae) and the integration of biopesticides with other biological approaches for their management with a focus on the pacific region. Insects 6, 297–318.10.3390/insects6020297CrossRefGoogle ScholarPubMed
Vivekanandhan, P, Swathy, K, Murugan, AC and Krutmuang, P (2022) Insecticidal efficacy of Metarhizium anisopliae derived chemical constituents against disease-vector mosquitoes. Journal of Fungi 8, 300.CrossRefGoogle ScholarPubMed
Vontas, J, Hernández-Crespo, P, Margaritopoulos, JT, Ortego, F, Feng, HT, Mathiopoulos, KD and Hsu, JC (2011) Insecticide resistance in tephritid flies. Pesticide Biochemistry and Physiology 100, 199–205.CrossRefGoogle Scholar
Wang, CS and St Leger, RJ (2007) The MAD1 adhesin of Metarhizium anisopliae links adhesion with blastospore production and virulence to insects, and the MAD2 adhesin enables attachment to plants. Eukaryotic Cell 6, 808–816.10.1128/EC.00409-06CrossRefGoogle ScholarPubMed
Wang, CS and Wang, SB (2017) Insect pathogenic fungi: Genomics, molecular interactions, and genetic improvements. Annual Review of Entomology 62, 73–90.10.1146/annurev-ento-031616-035509CrossRefGoogle ScholarPubMed
Wang, JJ, Wei, D, Dou, W, Hu, F, Liu, WF and Wang, JJ (2013) Toxicities and synergistic effects of several insecticides against the oriental fruit fly (Diptera: Tephritidae). Journal of Economic Entomology 106, 970–978.10.1603/EC12434CrossRefGoogle Scholar
Wasuwan, R, Phosrithong, N, Promdonkoy, B, Sangsrakru, D, Sonthirod, C, Tangphatsornruang, S, Likhitrattanapisal, S, Ingsriswang, S, Srisuksam, C, Klamchao, K, Suksangpanomrung, M, Hleepongpanich, T, Reungpatthanaphong, S, Tanticharoen, M and Amnuaykanjanasin, A (2021) The fungus Metarhizium sp. BCC 4849 is an effective and safe mycoinsecticide for the management of spider mites and other insect pests. Insects 13, 42.CrossRefGoogle Scholar
Zhang, XC, Li, XX, Gong, YW, Li, YR, Zhang, KL, Huang, YH and Zhang, F (2018) Isolation, identification, and virulence of a new Metarhizium anisopliae strain on the German cockroach. Pesticide Biochemistry and Physiology 111, 2611–2616.Google ScholarPubMed
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