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Microsatellites reveal genetic differentiation among populations in an insect species with high genetic variability in dispersal, the codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae)

Published online by Cambridge University Press:  14 April 2009

M.H. Chen  and
S. Dorn
M.H. Chen
Affiliation:
ETH Zurich, Institute of Plant Sciences/Applied Entomology, Schmelzbergstrasse 9/LFO, CH-8092, Zurich, Switzerland
S. Dorn*
Affiliation:
ETH Zurich, Institute of Plant Sciences/Applied Entomology, Schmelzbergstrasse 9/LFO, CH-8092, Zurich, Switzerland
*
*Author for correspondence Fax: +41 44 632 1171 E-mail: silvia.dorn@ipw.agrl.ethz.ch
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Abstract

Little is known about genetic differentiation and gene flow in populations of insect species that have a high genetic variability in dispersal but lack morphologically visible morphs that disperse. These characteristics apply to the codling moth, Cydia pomonella L. (Lepidoptera: Tortricidae), a major pest of fruits and nuts. Larvae were collected from three orchards each of pome fruits, stone fruits and nut trees in a major fruit growing area of Switzerland (Valais) and from six further (mainly apple) orchards throughout this country. Nine microsatellite loci were used to investigate genetic differentiation and the amount of gene flow among the sampled populations. All the loci were shown to be polymorphic in all populations. The number of alleles ranged from five to 15 over nine loci for the 15 populations. Significant genetic differentiation was noted among the populations from apple, apricot and walnut in the Valais region. Furthermore, among the eight populations sampled from apple in different geographic regions throughout Switzerland, AMOVA and pairwise FST analysis revealed significant population genetic differentiation even between populations collected from orchards ≪10 km apart. These results indicate that a distinct prevailing characteristic, in the present case the sedentary behaviour of the moth, can shape population architecture.

Keywords

Cydia pomonellagenetic differentiationmicrosatellitemetapopulationhost-plant racesisolation
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 100 , Issue 1 , February 2010 , pp. 75 - 85
Copyright
Copyright © Cambridge University Press 2009

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