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Identification of mealybug pest species (Hemiptera: Pseudococcidae) in Egypt and France, using a DNA barcoding approach

Published online by Cambridge University Press:  24 February 2012

S. Abd-Rabou ,
H. Shalaby ,
J.-F. Germain ,
N. Ris ,
P. Kreiter  and
T. Malausa
S. Abd-Rabou
Affiliation:
Agricultural Research Center, Plant Protection Research Institute, Dokki, Giza, Egypt
H. Shalaby
Affiliation:
Agricultural Research Center, Plant Protection Research Institute, Dokki, Giza, Egypt
J.-F. Germain
Affiliation:
ANSES, Laboratoire de la Santé des végétaux, Unité d'entomologie et plantes invasives, Campus international de Baillarguet, CS 30016, 34988 Montferrier-sur-Lez Cedex, France
N. Ris
Affiliation:
Institut National de la Recherche Agronomique, UMR ISA INRA/UNSA/CNRS, BPI and RDLB teams, 400, route des Chappes, BP 167, 06903 Sophia-Antipolis Cedex, France
P. Kreiter
Affiliation:
Institut National de la Recherche Agronomique, UMR ISA INRA/UNSA/CNRS, BPI and RDLB teams, 400, route des Chappes, BP 167, 06903 Sophia-Antipolis Cedex, France
T. Malausa*
Affiliation:
Institut National de la Recherche Agronomique, UMR ISA INRA/UNSA/CNRS, BPI and RDLB teams, 400, route des Chappes, BP 167, 06903 Sophia-Antipolis Cedex, France
*
*Author for correspondence Fax: +33 4 92 38 64 01 E-mail: tmalausa@sophia.inra.fr
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Abstract

Pseudococcidae (mealybugs) is a large taxonomic group, including a number of agronomic pests. Taxonomic identification of mealybug species is a recurrent problem and represents a major barrier to the establishment of adequate pest management strategies. We combined molecular analysis of three DNA markers (28S-D2, cytochrome oxidase I and internal transcribed spacer 2) with morphological examination, for the identification of 176 specimens collected from 40 mealybug populations infesting various crops and ornamental plants in Egypt and France. This combination of DNA and morphological analyses led to the identification of 17 species: seven in Egypt (Planococcus citri (Risso), Planococcus ficus (Signoret), Maconellicoccus hirsutus (Green), Ferrisia virgata (Cockerell), Phenacoccus solenopsis Tinsley, Phenacoccus parvus Morrison and Saccharicoccus sacchari (Cockerell)) and 11 in France (Planococcus citri, Pseudococcus viburni Signoret, Pseudococcus longispinus (Targioni-Tozzetti), Pseudococcus comstocki (Kuwana), Rhizoecus amorphophalli Betrem, Trionymus bambusae (Green), Balanococcus diminutus (Leonardi), Phenacoccus madeirensis Green, Planococcus vovae (Nasonov), Dysmicoccus brevipes (Cockerell) and Phenacoccus aceris Signoret), Pl. citri being found in both countries. We also found genetic variation between populations considered to belong to the same species, justifying further investigation of the possible occurrence of complexes of cryptic taxa.

Keywords

DNA barcodesPseudococcidaemitochondrial DNAnuclear DNACOIITS228Staxonomic identificationvoucher slide-mounted specimens

Information

Type
Research Paper
Information
Bulletin of Entomological Research , Volume 102 , Issue 5 , October 2012 , pp. 515 - 523
Copyright
Copyright © Cambridge University Press 2012

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