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The identification and expression analysis of candidate chemosensory genes in the bird cherry-oat aphid Rhopalosiphum padi (L.)

Published online by Cambridge University Press:  04 December 2017

Z.-W. Kang ,
F.-H. Liu ,
R.-P. Pang ,
W.-B. Yu ,
X.-L. Tan ,
Z.-Q. Zheng ,
H.-G. Tian  and
T.-X. Liu
Z.-W. Kang
Affiliation:
State Key Laboratory of Crop Stress Biology for the Arid Areas, and Key Laboratory of Northwest Loess Plateau Crop Pest Management of Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, China
F.-H. Liu
Affiliation:
State Key Laboratory of Integrated Management of Pest and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China
R.-P. Pang
Affiliation:
State Key Laboratory of Crop Stress Biology for the Arid Areas, and Key Laboratory of Northwest Loess Plateau Crop Pest Management of Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, China
W.-B. Yu
Affiliation:
State Key Laboratory of Crop Stress Biology for the Arid Areas, and Key Laboratory of Northwest Loess Plateau Crop Pest Management of Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, China
X.-L. Tan
Affiliation:
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
Z.-Q. Zheng
Affiliation:
State Key Laboratory of Crop Stress Biology for the Arid Areas, and Key Laboratory of Northwest Loess Plateau Crop Pest Management of Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, China
H.-G. Tian*
Affiliation:
State Key Laboratory of Crop Stress Biology for the Arid Areas, and Key Laboratory of Northwest Loess Plateau Crop Pest Management of Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, China
T.-X. Liu*
Affiliation:
State Key Laboratory of Crop Stress Biology for the Arid Areas, and Key Laboratory of Northwest Loess Plateau Crop Pest Management of Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, China
*
*Author for correspondence Tel/Fax: +86 29 87092663 E-mail: tianhg@nwsuaf.edu.cn; txliu@nwsuaf.edu.cn
*Author for correspondence Tel/Fax: +86 29 87092663 E-mail: tianhg@nwsuaf.edu.cn; txliu@nwsuaf.edu.cn
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Abstract

The bird cherry-oat aphid Rhopalosiphum padi (L.) is one of the most important wheat pests with polyphagia and autumn migrants. And, chemosensory genes were thought to play a key role in insect searching their hosts, food and mate. However, a systematic identification of the chemosensory genes in this pest has not been reported. Thus, in this study, we identified 14 odorant-binding proteins, nine chemosensory proteins, one sensory neuron membrane protein, 15 odorant receptors, 19 gustatory receptors and 16 ionotropic receptors from R. padi transcriptomes with a significantly similarity (E-value < 10−5) to known chemosensory genes in Acyrthosiphon pisum and Aphis gossypii. In addition, real-time quantitative polymerase chain reaction (RT-qPCR) was employed to determine the expression profiles of obtained genes. Among these obtained genes, we selected 23 chemosensory genes to analyze their expression patterns in different tissues, wing morphs and host plants. We found that except RpOBP1, RpOBP3, RpOBP4 and RpOBP5, the rest of the selected genes were highly expressed in the head with antennae compared with body without head and antennae. Besides that, the stimulation and depression of chemosensory genes by plant switch indicated that chemosensory genes might be involved in the plant suitability assessment. These results not only provide insights for the potential roles of chemosensory genes in plant search and perception of R. padi but also provide initial background information for the further research on the molecular mechanism of the polyphagia and autumn migrants of it. Furthermore, these chemosensory genes are also the candidate targets for pest management control in future.

Keywords

Rhopalosiphum paditranscriptomechemosensory genephylogenetic analysisexpression pattern

Information

Type
Research Papers
Information
Bulletin of Entomological Research , Volume 108 , Issue 5 , October 2018 , pp. 645 - 657
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Copyright © Cambridge University Press 2017 

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