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Characterization of the M918T sodium channel gene mutation associated with strong resistance to pyrethroid insecticides in the peach-potato aphid, Myzus persicae (Sulzer)

Published online by Cambridge University Press:  13 December 2007

I. Eleftherianos ,
S.P. Foster ,
M.S. Williamson  and
I. Denholm
I. Eleftherianos*
Affiliation:
Division of Plant and Invertebrate Ecology, Rothamsted Research, Harpenden, Herts. AL5 2JQ, UK
S.P. Foster
Affiliation:
Division of Plant and Invertebrate Ecology, Rothamsted Research, Harpenden, Herts. AL5 2JQ, UK
M.S. Williamson
Affiliation:
Division of Biological Chemistry, Rothamsted Research, Harpenden, Herts. AL5 2JQ, UK
I. Denholm
Affiliation:
Division of Plant and Invertebrate Ecology, Rothamsted Research, Harpenden, Herts. AL5 2JQ, UK
*
*Author for correspondence Fax: +33 388 606 922 E-mail: Ioannis.Eleftherianos@ibmc.u-strasbg.fr
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Abstract

Recent advances in the characterisation of insect sodium channel gene sequences have identified a small number of point mutations within the channel protein that are implicated in conferring target-site resistance to pyrethroid insecticides (so-called knockdown resistance or kdr). The L1014F (leucine-to-phenylalanine) mutation located in the centre of segment 6 of the domain II region (IIS6) of the sodium channel (the so-called kdr trait) has been detected in the peach-potato aphid, Myzus persicae (Sulzer), and is considered to be the primary cause of pyrethroid resistance in this species. Here we report on the characterisation of a second mutation, M918T (methione-to-threonine), within the nearby IIS4–S5 intracellular linker (the so-called super-kdr trait) in a field clone also possessing L1014F, with both mutations present in heterozygous form. The resistance phenotype of M. persicae clones possessing various combinations of L1014F and M918T to a wide range of pyrethroids (both Type I and II) was assessed in leaf-dip bioassays and to lambda-cyhalothrin applied at up to ten times the recommended field rate as foliar sprays to aphids feeding on whole plants. Bioassay results demonstrated that presence of both mutations was associated with extreme resistance to all the pyrethroids tested relative to aphids lacking the mutations. Furthermore, this resistance well exceeded that shown by aphids that were homozygous for L1014F but lacking M918T. However, pre-treatment with piperonyl butoxide in the leaf-dip bioassays failed to suppress pyrethroid resistance in aphids carrying one or both of the mutations. The relevance of these findings for monitoring and managing pyrethroid resistance in M. persicae populations in the field is discussed.

Keywords

Myzus persicaepyrethroidssodium channelknockdown resistancesuper-kdr mutation
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 98 , Issue 2 , April 2008 , pp. 183 - 191
Copyright
Copyright © Cambridge University Press 2007

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