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Mating behaviour and cholesterol nutritional strategies promoted ovarian development of female swimming crab (Portunus trituberculatus)

Published online by Cambridge University Press:  11 October 2024

Tingting Zhu ,
Min Jin Open the ORCID record for Min Jin [Opens in a new window] ,
Jiaxiang Luo ,
Yuhang Yang ,
Xiangkai Li ,
Hongyu Peng ,
Yuedong Shen  and
Qicun Zhou Open the ORCID record for Qicun Zhou [Opens in a new window]
Tingting Zhu
Affiliation:
Laboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, People’s Republic of China Key Laboratory of Aquaculture Biotechnology Ministry of Education, Ningbo University, Ningbo 315211, People’s Republic of China Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo 315211, People’s Republic of China
Min Jin*
Affiliation:
Laboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, People’s Republic of China Key Laboratory of Aquaculture Biotechnology Ministry of Education, Ningbo University, Ningbo 315211, People’s Republic of China Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo 315211, People’s Republic of China
Jiaxiang Luo
Affiliation:
Laboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, People’s Republic of China Key Laboratory of Aquaculture Biotechnology Ministry of Education, Ningbo University, Ningbo 315211, People’s Republic of China Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo 315211, People’s Republic of China
Yuhang Yang
Affiliation:
Laboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, People’s Republic of China Key Laboratory of Aquaculture Biotechnology Ministry of Education, Ningbo University, Ningbo 315211, People’s Republic of China Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo 315211, People’s Republic of China
Xiangkai Li
Affiliation:
Laboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, People’s Republic of China Key Laboratory of Aquaculture Biotechnology Ministry of Education, Ningbo University, Ningbo 315211, People’s Republic of China Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo 315211, People’s Republic of China
Hongyu Peng
Affiliation:
Laboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, People’s Republic of China Key Laboratory of Aquaculture Biotechnology Ministry of Education, Ningbo University, Ningbo 315211, People’s Republic of China Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo 315211, People’s Republic of China
Yuedong Shen
Affiliation:
Laboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, People’s Republic of China Key Laboratory of Aquaculture Biotechnology Ministry of Education, Ningbo University, Ningbo 315211, People’s Republic of China Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo 315211, People’s Republic of China
Qicun Zhou*
Affiliation:
Laboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, People’s Republic of China Key Laboratory of Aquaculture Biotechnology Ministry of Education, Ningbo University, Ningbo 315211, People’s Republic of China Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo 315211, People’s Republic of China
*
*Corresponding authors: Min Jin, email jinmin@nbu.edu.cn; Qicun Zhou, email zhouqicun@nbu.edu.cn
*Corresponding authors: Min Jin, email jinmin@nbu.edu.cn; Qicun Zhou, email zhouqicun@nbu.edu.cn
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Abstract

Female crabs enter a stage of rapid ovarian development after mating, and cholesterol is a substrate for steroid hormone synthesis. Therefore, in this experiment, an 8-week feeding trial was conducted to investigate the effects of mating treatments (mated crab and unmated crab) and three dietary cholesterol levels (0·09 %, 0·79 % and 1·40 %) on ovarian development, cholesterol metabolism and steroid hormones metabolism of adult female swimming crab (Portunus trituberculatus). The results indicated that crabs fed the diet with 0·79 % cholesterol significantly increased gonadosomatic index (GSI) and vitellogenin (VTG) content than other treatments in the same mating status. Moreover, mated crabs had markedly increased GSI and VTG content in the ovary and hepatopancreas than unmated crabs. The histological observation found that exogenous vitellogenic oocytes appeared in the mated crabs, while previtellogenic oocytes and endogenous vitellogenic oocytes were the primary oocytes in unmated crabs. The transmission electron microscopy analysis showed that when fed diet with 0·79 % cholesterol, the unmated crabs contained more rough endoplasmic reticulum and mated crabs had higher yolk content than other treatments. Furthermore, mating treatment and dietary 0·79 % cholesterol level both promoted cholesterol deposition by up-regulation of the mRNA and protein expression levels of class B scavenger receptors 1 (Srb1), while stimulating the secretion of steroid hormones by up-regulation of the mRNA and protein expression of steroidogenic acute regulatory protein (Star). Overall, the present results indicated that mating behaviour plays a leading role in promoting ovarian development, and dietary 0·79 % cholesterol level can further promote ovarian development after mating.

Keywords

Portunus trituberculatusMatingCholesterolOvarian developmentSteroid hormones
Type
Research Article
Information
British Journal of Nutrition , Volume 132 , Issue 7 , 14 October 2024 , pp. 835 - 850
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Copyright
© The Author(s), 2024. Published by Cambridge University Press on behalf of The Nutrition Society

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