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Interactions between vitamins C and E in human subjects

Published online by Cambridge University Press:  09 March 2007

Iona M. J. Hamilton ,
William S. Gilmore ,
Iris F. F. Benzie ,
Clive W. Mulholland  and
J. J. Strain
Iona M. J. Hamilton
Affiliation:
Northern Ireland Centre for Diet and Health (NICHE), University of Ulster, Coleraine BT52 1SA, Northern Ireland, UK
William S. Gilmore
Affiliation:
Northern Ireland Centre for Diet and Health (NICHE), University of Ulster, Coleraine BT52 1SA, Northern Ireland, UK
Iris F. F. Benzie
Affiliation:
Department of Nursing and Health Sciences, The Hong Kong Polytechnic University, Kowloon, Hong Kong
Clive W. Mulholland
Affiliation:
Northern Ireland Centre for Diet and Health (NICHE), University of Ulster, Coleraine BT52 1SA, Northern Ireland, UK
J. J. Strain*
Affiliation:
Northern Ireland Centre for Diet and Health (NICHE), University of Ulster, Coleraine BT52 1SA, Northern Ireland, UK
*
*Corresponding author: Professor J. J. Strain, fax +44 1265 324965, email J.J.Strain@ulst.ac.uk
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Abstract

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Despite convincing in vitro evidence, a vitamin C–E interaction has not been confirmed in vivo. This study was designed to examine the effects of supplementation with either vitamin C or E on their respective plasma concentrations, other antioxidants, lipids and some haemostatic variables. Fasting blood was collected before and after intervention from thirty healthy adults in a double-blinded crossover study. Baselines for measured variables were established after 2 weeks of placebo supplementation, followed by daily supplementation with 73·5 mg RRR-α-tocopherol acetate or 500 mg ascorbic acid, and placebo, for 6 weeks. A 2 month washout preceded supplement crossover. Mean values showed that plasma lipid standardised α-tocopherol increased with ascorbic acid supplementation: from 4·09 (SEM 0·51) TO 4·53 (sem 0·66) μmol/mmol total cholesterol plus triacylglycerol (P < 0·05), and plasma ascorbic acid increased from 62·8 (sem 14·9) to 101·3 (sem 22·2) μmol/l (P < 0·005). Supplementation with (RRR)-α-tocopherol acetate increased plasma α-tocopherol from 26·8 (sem 3·9) to 32·2 (sem 3·8) μmol/l (P < 0·05), and lipid-standardised α-tocopherol from 4·12 (sem 0·48) to 5·38 (sem 0·52) μmol/mmol (P < 0·001). Mean plasma ascorbic acid also increased with vitamin E supplementation, from 64·4 (sem 13·3) to 76·4 (sem 18·4) μmol/l (P < 0·05). Plasma ferric reducing (antioxidant) power and glutathione peroxidase (U/g haemoglobin) increased in both groups, while urate, total cholesterol and triacylglycerol levels decreased (P < 0·05 throughout). Results are supportive of an in vivo interaction between vitamins C and E.

Keywords

AntioxidantsVitamin CVitamin E

Information

Type
Research Article
Information
British Journal of Nutrition , Volume 84 , Issue 3 , September 2000 , pp. 261 - 267
Copyright
Copyright © The Nutrition Society 2000

References

Armstrong, AM, Chestnut, JE, Cromley, MJ and Young, IS (1996) The effect of dietary treatment on lipid peroxidation and antioxidant status in newly diagnosed non-insulin dependent diabetes. Free Radical Biology and Medicine 21, 719–726.CrossRefGoogle Scholar
Austin, MA, Stephens, K, Walder, CE and Wijisman, E (1999) Linkage analysis of candidate genes and the small dense low-density lipoprotein phenotype. Atherosclerosis 142, 79–87.CrossRefGoogle ScholarPubMed
Bain, BJ (1995) Method for the determination of haemoglobin concentration in blood. In Blood Cells: A Practical Approach pp. 15–18 [Bain, BJ, editor]. Oxford: Blackwell Science.Google Scholar
Benzie, IFF and Strain, JJ (1996) Uric acid, friend or foe?. Redox Report 2, 231–234.CrossRefGoogle ScholarPubMed
Benzie, IFF and Strain, JJ (1997) Acute post-ingestion changes in plasma ascorbic acid concentration. Relationship to dose and to existing body stores. Nutrition Research 17, 2187–2190.CrossRefGoogle Scholar
Benzie, IFF and Strain, JJ (1997) Simultaneous automated measurement of total antioxidant (reducing) capacity and ascorbic acid concentration. Redox Report 3, 322–328.CrossRefGoogle ScholarPubMed
Benzie, IFF and Strain, JJ (1999) Effect of vitamin C supplementation on concentrations of vitamins C and E in fasting plasma. Asia Pacific Journal of Clinical Nutrition 8, 207–210.CrossRefGoogle Scholar
Benzie, IFF & Strain, JJ (1999b) Ferric reducing (antioxidant) power as a measure of antioxidant capacity: the FRAP Assay. In Oxidants and Antioxidants: A Volume of Methods in Enzymology, pp. 15–27 [Packer, L, editor]. Orlando, FL: Academic Press.CrossRefGoogle Scholar
Chan, AC (1993) Partners in defense Vitamin C and Vitamin E?. Canadian Journal of Physiology and Pharmacy 719, 727–731.Google Scholar
Chen, MF, Hon, HC, Luai, CS and Lee, YT (1995) Vitamin E supplementation attenuates the myointimal proliferation of the abdominal aorta after balloon injury in diet-induced hypercholesterolemic rabbits. Prostaglandins 56, 219–238.CrossRefGoogle Scholar
Choc, CK (1991) Vitamins and related dietary antioxidants. In Trace Elements, Micronutrients and Free Radicals, pp. 129–147 [Droesti, IE, editor]. New Jersey: Humana Press.Google Scholar
Clazada, C, Bruckdorfer, KR and Rice-Evans, CA (1997) The influence of antioxidant nutrients on platelet function of healthy volunteers. Atherosclerosis 128, 97–105.CrossRefGoogle Scholar
Constantinezcu, A, Han, D and Packer, L (1993) Vitamin E recycling in human erythrocyte membranes. Journal of Biology and Chemistry 268, 10906–10913.CrossRefGoogle Scholar
DiSciascio, N, Quarantino, CP, Rucci, C, Ciaglia, P, Mariari, MN and Giacomello, A (1994) Correlation of serum uric acid, cholesterol and triacylglycerol levels. Advances in Experimental Medicine and Biology 370, 77–78.CrossRefGoogle Scholar
Fernandez, ML, Vega, S, Ayala, MT, Shen, H, Conde, K, Vergara-Jimenez, M and Rolskins, A (1997) Vitamin C level and dietary fat saturation alter hepatic cholesterol metabolism and plasma low-density lipoprotein metabolism in guinea-pigs. Journal of Nutritional Biochemistry 8, 414–424.CrossRefGoogle Scholar
Feskens, EJM, Virtaneu, SM, Rasanen, L, Tuomilelito, J, Stengard, J, Pekkanen, J, Nissinen, A and Kromhout, D (1995) Dietary factors determining diabetes and impaired glucose tolerance — a 20-year follow-up of the Finnish and Dutch cohorts of the Seven Countries Study. Diabetes Care 18, 1104–1112.CrossRefGoogle ScholarPubMed
Frei, B, England, L and Ames, BN (1989) Ascorbate is an outstanding antioxidant in human blood plasma. Proceedings of the National Academy of Science 86, 6371–6381.CrossRefGoogle ScholarPubMed
Gey, KF (1998) Vitamins E plus C and interacting constituents required for optimal health. A critical and constructive review of epidemiology and supplementation data regarding cardiovascular disease and cancer. Biofactors 7, 113–174.Google Scholar
Gey, KF and Puska, P (1989) Plasma vitamins E and A inversely correlated to mortality from IHD in cross-cultural epidemiology. Annals of the New York Academy of Science 570, 268–282.CrossRefGoogle Scholar
Ginter, E, Jurcovicova, M and Chronin, C (1987) Vitamin C deficiency lowers the fractional catabolic rate of low-density lipoproteins in guinea-pigs. Annals of the New York Academy of Science 498, 473–497.CrossRefGoogle Scholar
Hallfrisch, J, Singh, NN, Muller, DC, Baldwin, H, Bannon, ME and Andres, R (1994) High plasma vitamin C associated with high plasma HDL and HDL-2 cholesterol. American Journal of Clinical Nutrition 60, 100–105.CrossRefGoogle Scholar
Halpner, AD, Hanndelman, GJ, Belmont, CA, Harris, JM and Blumberg, JB (1998) Protection by vitamin C of oxidant-induced loss of vitamin E in rat hepatocytes. Journal of Nutritional Biochemistry 9, 355–359.CrossRefGoogle Scholar
Jacob, RA (1995) The integrated antioxidant system. Nutrition Research 15, 755–766.CrossRefGoogle Scholar
Jari, AE, Karlstrom, BE, Grangfeldt, YE, Bjork, IE, Asp, NGL and Vessby, BOH (1999) Improved glycaemic control and lipid profile and normalised fibrinolytic activity on a low-glycaemic index diet in type 2 diabetic patients. Diabetes Care 22, 10–18.CrossRefGoogle Scholar
Johnson, DJ, Kwlighin, SD, Kim, YG, Suga, S and Fogo, AB (1999) Reappraisal of the pathogenesis and consequences of hyperuricaemia in hypertension, cardiovascular disease and renal disease. American Journal of Kidney Diseases 33, 225–234.CrossRefGoogle ScholarPubMed
Khaw, KT and Woodhouse, P (1997) Infection, haemostatic factors and cardiovascular disease. Fibrinolysis and Proteolysis 11, S149–S153.CrossRefGoogle Scholar
Lee, J, Sparrow, D, Vokonas, PS, Landsberg, L and Weiss, ST (1995) Uric acid and coronary heart disease risk; evidence for a role for uric acid in obesity–insulin resistance syndrome. American Journal of Epidemiology 142, 288–294.CrossRefGoogle ScholarPubMed
Lim, JF and Lee, YW (1998) Vitamin C supplementation restores the impaired vitamin E status of guinea-pigs fed oxidised frying oil. Journal of Nutrition 128, 116–122.Google Scholar
Livingstone, MBE, Prentice, AM, Coward, WA, Strain, JJ, Black, AE, Davies, PSW, Stewart, CM, Mc Kenna, PG and Whitehead, RG (1992) Validation of estimates of energy intake by weighed dietary record and diet history in children and adolescents. American Journal of Clinical Nutrition 56, 29–35.CrossRefGoogle ScholarPubMed
May, JM, Qu, Z and Mendiratta, S (1998) Protection and recycling of α-tocopherol in human erythrocytes by intracellular ascorbic acid. Archives of Biochemistry and Biophysics 349, 281–289.CrossRefGoogle ScholarPubMed
Niki, E, Noguchi, N, Tsuchiharski, H and Gotoh, N (1995) Interaction among vitamin C, vitamin E and B-carotene. American Journal of Clinical Nutrition 6, S1322–S1326.CrossRefGoogle Scholar
Paolisso, G, Balbi, V, Volpe, C, Varricchio, G, Gambardella, A, Saccomanno, F, Ammendola, S, Varricchio, M and Doisfrio, F (1995) Metabolic benefits deriving from chronic vitamin C supplementation in aged non-insulin dependent diabetic mellitus. Journal of the American College of Nutrition 14, 387–392.CrossRefGoogle Scholar
Peuchant, E, Delmasbeauvieux, MC, Couchouron, A, Dubourg, L, Thomas, MJ, Perromat, A, Clerc, M and Oln, H (1997) Short-term insulin therapy and normoglycaemic effects on erythrocyte lipid peroxidation in NIDDM patients. Diabetes Care 20, 202–207.CrossRefGoogle Scholar
Rimm, EB, Stampfer, MJ, Ascherio, A, Giovannincci, E, Colditz, GA and Willett, WC (1993) Vitamin E consumption and the risk of coronary heart disease in men. New England Journal of Medicine 328, 1450–1456.CrossRefGoogle ScholarPubMed
Rock, CL, Fada, RD, Jacob, RA and Rowen, PE (1996) Update on the biological characteristics of the antioxidant micronutrients: vitamin C, vitamin E and the carotenoids. Journal of the American Dietietic Association 96, 693–702.CrossRefGoogle ScholarPubMed
Samman, S, Brown, AJ and Singh, S (1997) The effect of ascorbic acid on plasma lipids and oxidisibility of LDL in male smokers. European Journal of Clinical Nutrition 51, 472–477.CrossRefGoogle Scholar
Schlotte, V, Sevanian, A, Hochstein, P and Weithman, KM (1998) Effect of uric acid and chemical analogues on oxidation of human low density lipoprotein. in vitro Free Radicals in Biology and Medicine 25, 839–847.CrossRefGoogle ScholarPubMed
Schmenke, DC and Behr, SR (1998) Vitamin E combined with selenium inhibits atherosclerosis in hypercholesterolemic rabbits independent of effects on plasma cholesterol concentrations. Circulation Research 8, 366–371.CrossRefGoogle Scholar
Shaheen, AA, Abdelfattah, AA and Siefelvasr, M (1996) Influence of verapramil on the efficacy of vitamin E in preventing the ischaemia-reperfusion induced biochemical derangement in cerebral cortex of rat. Drug Research 47, 670–673.Google Scholar
Sharma, MK and Buettner, GR (1993) Interaction of vitamin C and vitamin E during free radical stress in plasma — an ESR study. Free Radicals in Biology and Medicine 14, 649–653.CrossRefGoogle ScholarPubMed
Sprenger, T, Finckh, B, Fingerhut, R, Kohlschutter, A, Biesiegel, U and Kontush, A (1998) How different constituents of human plasma and low density lipoprotein determine plasma oxidisability by copper. Chemistry and Physics of Lipids 91, 39–52.CrossRefGoogle Scholar
Stoyanovsky, DA, Goldman, R, Darrow, RM, Organisciak, DT and Kagan, VE (1995) Endogenous ascorbic acid regenerates vitamin E in the retina directly and in combination with exogenous dihydrolipoic acid. Current Eye Research 14, 181–189.CrossRefGoogle ScholarPubMed
Strain, JJ & Benzie, IFF (1999) Antioxidants/diet and antioxidant defence. In Encyclopedia of Human Nutrition, vol. 1, pp. 95–106 [Sadler, M, Strain, JJ and Caballero, B, editors]. London: Academic Press.Google Scholar
Strain, JJ & Mulholland, CW (1992) Vitamin C and Vitamin E-synergistic interactions in vivo? In Free Radicals and Aging, pp. 419–422 [Emerit, I and Chance, B, editors]. Switzerland: Birkhauser Verlag.CrossRefGoogle Scholar
Tanaka, K, Hashimoto, T, Tokumaru, S, Iguchi, I and Kojo, S (1997) Interactions between vitamin C and vitamin E are observed in tissues of inherently scorbutic rats. Journal of Nutrition 127, 2060–2064.CrossRefGoogle ScholarPubMed
Thomas, SR, Neuzil, J, Mohr, D and Stocker, R (1995) Restoration of tocopherol by co-antioxidants make α-tocopherol an effective antioxidant for low-density lipoproteins. American Journal of Clinical Nutrition 62, S1357–S1364.CrossRefGoogle Scholar
Thurnham, DI, Davis, JA, Crump, BJ, Situayake, RD and Davis, M (1986) The use of different lipids to express serum tocopherol: lipid ratios for the measurement of vitamin E status. Annals of Clinical Biochemistry 23, 514–517.CrossRefGoogle ScholarPubMed
Thurnham, DI, Smith, E and Flora, PS (1988) Concurrent liquid chromatographic assay of retinol, α-tocopherol, β-carotene, α-carotene, lycopene and β-cryptoxanthine in plasma with tocopherol acetate as an internal standard. Clinical Chemistry 34, 377–381.CrossRefGoogle Scholar
Torun, M, Yardin, S, Sinusek, B and Burgaz, S (1998) Serum uric acid levels in cardiovascular diseases. Journal of Clinical and Pharmacological Therapeutics 23, 25–29.CrossRefGoogle ScholarPubMed
Vasankari, TJ, Kujala, UM, Vasankari, TM, Vuorimara, T and Ahotupa, M (1997) Increased serum and LDL antioxidant potential after supplementation in endurance athletes. American Journal of Clinical Nutrition 65, 1052–1056.CrossRefGoogle ScholarPubMed
Verlangierii, AJ and Bush, MJ (1992) Effects of α-tocopherol supplementation on experimentally induced primate atherosclerosis. Journal of the American College of Nutrition 11, 130–137.CrossRefGoogle Scholar
Willingham, AK, Blanos, C, Bohannan, E and Candella, RJ (1993) The effects of high levels of vitamin E on the progression of atherosclerosis in the Watanabe heritable hyperlipemic rabbit. Journal of Nutritional Biochemistry 4, 651–654.CrossRefGoogle Scholar
Witting, LA (1995) Vitamin E Do we need it? In Nutrition and Health. Topics and Controversies, pp. 157–179 [Wolinsky, I and Hickson, JF, editors]. Boca Raton, FL: CRC Press.Google Scholar
Yarnell, J, Baker, IA, Sweetman, PM, Bainton, D, O'Brien, JR, Whitehead, PJ and Elwood, PC (1991) Fibrinogen, viscosity and white blood cell count are major risk factors for ischaemic heart disease, the Caerphilly and Speedwell collaborative heart studies. Circulation 83, 36–44.CrossRefGoogle Scholar