Exhaustive physical exercise can impact intestinal health, affecting permeability, inflammation, and the production of short-chain fatty acids (SCFA). Dietary modifications, such as the consumption of whey protein concentrate (WPC) and curcumin (CCM), can modulate these effects due to their anti-inflammatory and antioxidant properties. This study evaluated the impact of WPC+CCM and CCM in Wistar rats submitted to exhaustive exercise (EE). Forty-eight male Wistar rats (age:12 weeks) were randomly divided into 6 groups (n=8). After 4 weeks on diet, rats from EE groups were submitted to an exhaustive swimming test. Twenty-four hours later, animals from all experimental groups were euthanized, and had feces collected from the cecum. The colon was dissected for interest analysis. SCFA, oxidative stress, real-time polymerase chain reaction, and histomorphometry analyses were performed. The results showed that the SCFA content remained stable, malondialdehyde levels did not vary, but the WPC+CCM group showed higher carbonylated protein concentration. Nitric oxide decreased in the treated groups, while antioxidant enzymes increased in the WPC+CCM and CCM groups, except for glutathione, which decreased. The expression of Nrf2, NF-κB, and occludin were maintained, and the expression of claudin increased after physical stress with the consumption of WPC+CCM. CCM increased mucosal thickness and preserved goblet cells. In conclusion, WPC+CCM prevented increased oxidative stress and inflammation and preserved the production of SCFA, antioxidant activity, and intestinal integrity of rats after exhaustive exercise.