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Antihypertensive effect of casein hydrolysate in a placebo-controlled study in subjects with high-normal blood pressure and mild hypertension

Published online by Cambridge University Press:  08 March 2007

Seiichi Mizuno ,
Keiichi Matsuura ,
Takanobu Gotou ,
Shingo Nishimura ,
Osami Kajimoto ,
Mitsuharu Yabune ,
Yoshitaka Kajimoto  and
Naoyuki Yamamoto
Seiichi Mizuno
Affiliation:
Food Research Laboratory, Calpis Co. Ltd. Sagamihara, Kanagawa, Japan
Keiichi Matsuura
Affiliation:
Food Research Laboratory, Calpis Co. Ltd. Sagamihara, Kanagawa, Japan
Takanobu Gotou
Affiliation:
Food Research Laboratory, Calpis Co. Ltd. Sagamihara, Kanagawa, Japan
Shingo Nishimura
Affiliation:
Food Research Laboratory, Calpis Co. Ltd. Sagamihara, Kanagawa, Japan
Osami Kajimoto
Affiliation:
Soiken Inc., Toyonaka, Osaka, Japan
Mitsuharu Yabune
Affiliation:
Soiken Inc., Toyonaka, Osaka, Japan
Yoshitaka Kajimoto
Affiliation:
Soiken Inc., Toyonaka, Osaka, Japan
Naoyuki Yamamoto*
Affiliation:
Food Research Laboratory, Calpis Co. Ltd. Sagamihara, Kanagawa, Japan
*
*Corresponding author: Dr Naoyuki Yamamoto, fax +81 042 769 7810, email naoyuki.yamamoto@calpis.co.jp
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Abstract

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We describe a clinical trial to study the efficacy of a casein hydrolysate, prepared using an Aspergillus oryzae protease, containing the major angiotensin-I-converting enzyme inhibitory peptides Val-Pro-Pro (VPP) and Ile-Pro-Pro (IPP) in a single-blind, placebo-controlled study. A total of 131 volunteers with high-normal blood pressure and mild hypertension were randomly divided into four groups (n 32 or 33 in each group). Each volunteer was given two tablets containing four different dosages of VPP and IPP (VPP+IPP: 0, 1·8, 2·5 and 3·6 mg), daily for 6 weeks. A significant decrease in systolic blood pressure was observed at 6 weeks in the active group receiving 1·8 mg (P<0·01) VPP and IPP; in the active groups receiving either 2·5 mg or 3·6 mg, systolic blood pressure was decreased at both 3 weeks (P<0·05 and P<0·05) and 6 weeks (P<0·001 and P<0·0001) compared with systolic blood pressure measured before treatment. Changes in the systolic blood pressure after 6 weeks of treatment in the four groups were −1·7, −6·3, −6·7 and −10·1 mmHg, and these effects were dose dependent. In addition, a significant difference in systolic blood pressure between the placebo group and the VPP and IPP group receiving 3·6 mg was observed (P<0·001) by two-way ANOVA. The antihypertensive effect was greater in mildly hypertensive subjects (n 20 or 21 in each group) than in any of the other subjects. No significant change of diastolic blood pressure was observed for all the test groups, and no differences in diastolic blood pressure in the test sample groups compared with the placebo group were observed during the test period.

Keywords

Antihypertensive effectClinical studyCasein hydrolysateVPP and IPP
Type
Research Article
Information
British Journal of Nutrition , Volume 94 , Issue 1 , July 2005 , pp. 84 - 91
Copyright
Copyright © The Nutrition Society 2005

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