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Adaptation of lipid metabolism, tissue composition and flesh quality in gilthead sea bream (Sparus aurata) to the replacement of dietary fish oil by linseed and soyabean oils

Published online by Cambridge University Press:  09 March 2007

D. Menoyo ,
M. S. Izquierdo ,
L. Robaina ,
R. Ginés ,
C. J. Lopez-Bote  and
J. M. Bautista
D. Menoyo
Affiliation:
Departamento de Bioquímica y Biología Molecular IV, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 MadridSpain Departamento de Producción Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain
M. S. Izquierdo
Affiliation:
Grupo de Investigación en Acuicultura (ULPGC & ICCM), P. O. Box 56, 35200 Telde, Islas Canarias, Spain
L. Robaina
Affiliation:
Grupo de Investigación en Acuicultura (ULPGC & ICCM), P. O. Box 56, 35200 Telde, Islas Canarias, Spain
R. Ginés
Affiliation:
Grupo de Investigación en Acuicultura (ULPGC & ICCM), P. O. Box 56, 35200 Telde, Islas Canarias, Spain
C. J. Lopez-Bote
Affiliation:
Departamento de Producción Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain
J. M. Bautista*
Affiliation:
Departamento de Bioquímica y Biología Molecular IV, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 MadridSpain
*
*Corresponding author: fax +34 91 394 3824, Email jmbau@vet.ucm.es
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Abstract

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Linseed (LO) and soyabean (SO) oils were evaluated as fish-oil (FO) substitutes in the diets of marketable-sized gilthead sea bream (Sparus aurata). Practical diets were designed factorially with the lipid added as follows (%): FO 100, LO 60+FO 40, LO 80+FO 20, SO 60+FO 40, SO 80+FO 20. The effects of experimental diets on growth, fatty acids patterns in liver and muscle, flesh quality variables and activities of selected enzymes involved in lipid synthesis and catabolism were determined at the end of a 7-month trial. Fatty acid composition of liver and muscle generally reflected the fatty acid composition of the diets. The n−3 PUFA levels were significantly reduced by the inclusion of vegetable oils. This tendency was more pronounced for EPA than for docosahexaenoic acid. The n−3:n−6 fatty acid ratio reached the lowest values in fish fed the SO diets; this was associated with a higher liver lipid deposition. No differences were found in fillet texture and pH. However, under conditions of forced peroxidation, muscles from fish fed the SO diets had lower peroxidation levels. Vegetable oil substitution decreased lipogenesis in liver and this effect was greatest at the highest substitution level. In contrast, muscle β-oxidation enzymes had increased activities with vegetable oil substitution. Thus, the lower hepatic lipogenesis was correlated with an increased lipid utilisation in muscle. It is concluded that growth and lipid metabolism were affected by experimental diets.

Keywords

Sea breamVegetable oilLipid metabolismFlesh qualityEssential fatty acids
Type
Research Article
Information
British Journal of Nutrition , Volume 92 , Issue 1 , July 2004 , pp. 41 - 52
Copyright
Copyright © The Nutrition Society 2004

References

1Alexis, MFish meal and fish oil replaces in Mediterranean marine fish diets Cah Options Méditerr 1997 22, 183–204.Google Scholar
2Alvarez, MJ, López-Bote, CJ, Diez, A, Corraze, G, Arzel, J, Dias, J, Kaushik, SJ & Bautista, JMDietary fish oil and digestible protein modify susceptibility to lipid peroxidation in the muscle of rainbow trout (Oncorhynchus mykiss) and sea bass (Dicentrarchus labrax). Br J Nut 1998 80, 281–289.CrossRefGoogle ScholarPubMed
3Andersen, UB, Thomassen, MS & Rørå, AMBTexture properties of farmed rainbow trout (Onchorhynchus mykiss): effects of diet, muscle fat content and time of storage on ice. J Sci Food Agric 1997 74, 347–353.3.0.CO;2-F>CrossRefGoogle Scholar
4Bell, JG, Henderson, RJ, Tocher, DR, McGhee, F, Dick, JR, Porter, A, Smullen, RP & Sargent, JRReplacement of fish oil with rapeseed oil in diets of Atlantic salmon (Salmo salar) affects tissue lipid composition and hepatocyte fatty acid metabolism. J Nutr 2001 131, 1535–1543.CrossRefGoogle ScholarPubMed
5Bell, JG, Henderson, RJ, Tocher, DR, McGhee, F, Dick, JR, Porter, A, Smullen, RP & Sargent, JRSubstituting fish oil with crude palm oil in the diet of Atlantic salmon (Salmo salar) affects muscle fatty acid conposition and hepatic fatty acid metabolism. J Nutr 2002 132, 222–230.CrossRefGoogle Scholar
6Bjerkeng, B, Refstie, S, Fjalestad, KT, Storebakken, T, Rodbotten, M & Roem, AJQuality parameters of the flesh of Atlantic salmon (Salmo salar) as affected by dietary fat content and full-fat soybean meal as a partial substitute for fish meal in diet. Aquaculture 1997 157, 297–309.CrossRefGoogle Scholar
7Bjerkeng, B, Hatlen, B & Wathne, EDeposition of astaxanthin in fillets of Atlantic salmon (Salmo salar) fed diets with herring, capelin, sandeel, or Peruvian high PUFA oils. Aquaculture 1999 180, 307–319.Google Scholar
8Bonamusa, L, García de Frutos, P, Fernández, F & Baanante, IVFeatures of the lipid transport system of fish as demonstrated by studies on starvation in rainbow trout. J Comp Physiol B 1992 156, 497–502.Google Scholar
9Borderías, AJ, Lamua, M & Tejada, MTexture analysis of fish fillets and minced fish by both sensory and instrumental methods. J Food Technol 1983 18, 85–95.Google Scholar
10Bradford, MMA rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976 72, 248–254.CrossRefGoogle ScholarPubMed
11Bradshaw, RA – Noyes, BEL-3-Hydroxyacyl coenzyme A dehydrogenase from pig heart muscle. In Methods in Enzymologyvol. 35pp. 122–128 [Lowestein, JMeditor]. New YorkAcademic Press 1975.Google ScholarPubMed
12Caballero, MJ, Izquierdo, MS & Kjørsvik, EHistological alterations in liver of sea bream (Sparus aurata) caused by feeding for a short- or long-term with vegetable oils. Recovery of normal morphology after feeding fish oil as a solely lipid source. J Fish Dis 2004(In the Press).CrossRefGoogle Scholar
13Caballero, MJ, Obach, A, Rosenlund, G, Montero, D, Gisvold, M & Izquierdo, MSImpact of different dietary sources on growth, lipid digestibility, tissue fatty acid composition and histology of rainbow trout, Oncorhynchus mykiss. Aquaculture 2002 253, 253–271.CrossRefGoogle Scholar
14Christie, WWLipid Analysis. Oxford: Pergamon Press 1982Google Scholar
15Commission Internationale de l'Eclairage Colorimetry. Commission Internationale de l'Eclairage Publication no. 15. Vienna: Bureau Central de la CIE 1976.Google Scholar
16Clarke, SDPolyunsaturated fatty acid regulation of gene transcription: a mechanism to improve energy balance and insulin resistance. Br J Nutr 2000 83, Suppl. 1, S59–S66.Google Scholar
17Corraze, G, Larroquet, L & Medale, FAlimentation et dépôts lipidiques chez la truit arc-en-ciel, effect de la temperature délevage (Nutrition control of lipid deposition in rainbow trout: effect of rearing temperature). Prod Anim 1999 12, 249–256.Google Scholar
18Dias, J, Alvarez, MJ, Diez, A, Arzel, J, Corraze, G, Bautista, JM & Kaushik, SJRegulation of hepatic lipogenesis by dietary protein/energy in juvenile European seabass (Dicentrarchus labrax). Aquaculture 1998 161, 169–186.CrossRefGoogle Scholar
19Food and Agriculture Organization The State of World Fisheries and Aquaculture Rome: FAO 2002Google Scholar
20Folch, J, Lees, M & Sloane-Stanley, GHA simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 1957 226, 497–509.CrossRefGoogle ScholarPubMed
21Fox, CStudies on polyunsaturated fatty acid nutrition in the larvae of a marine fish – the herring (Clupea harengus L), PhD Thesis, Stirling University 1990.Google Scholar
22Frøyland, L, Lie, Ø & Berge, RKMitochondrial and peroxisomal β-oxidation capacities in various tissues from Atlantic salmon Salmo salar. Aquacult Nutr 2000 6, 85–89.CrossRefGoogle Scholar
23Frøyland, L, Madsen, L, Eckhoff, KM, Lie, Ø & Berge, RKCarnitine palmitoyltransferase I, carnitine palmitoyltransferase II, and acyl-CoA oxidase activities in Atlantic salmon (Salmo salar). Lipids 1998 33, 923–930.Google Scholar
24Harper, RD & Saggerson, EDSome aspects of fatty acid oxidation in isolated fat-cell mitochondria from ra. Biochem J 1975 152, 485–494.CrossRefGoogle Scholar
25Hemre, GI, Mommsen, TP & Krogdahl, ÅCarbohydrates in fish nutrition: effects on growth, glucose metabolism and hepatic enzymes. Aquacult Nutr 2002 8, 175–194.CrossRefGoogle Scholar
26Ibeas, C, Izquierdo, MS & Lorenzo, AEffect of different levels of n -3 highly unsaturated fatty acids on growth and fatty acid composition of juvenile gilthead seabream (Sparus aurata). Aquaculture 1994 127, 177–188.Google Scholar
27Izquierdo, MSEssential fatty acid requirements of marine fish larvae. Aquacult Nutr 1996 2, 183–191.CrossRefGoogle Scholar
28Izquierdo, MS, Socorro, J, Arantzamendi, L & Hernández-Cruz, CMRecent advances in lipid nutrition in fish larvae. Fish Physiol Biochem 2000 22, 97–107.CrossRefGoogle Scholar
29Izquierdo, MS, Watanabe, T, Takeuchi, T, Arawaka, T & Kitajima, CRequirement of larval red sea bream Pagrus major for essential fatty acids. Bull Japanese Soc Sci Fish 1989 55, 859–867.Google Scholar
30Kalogeropoulos, N, Alexis, MN & Henderson, RJEffects of dietary soybean and cod-liver oil levels on growth and body composition of gilthead bream (Sparus aurata). Aquaculture 1992 104, 293–308.Google Scholar
31Kornbrust, DJ & Mavis, RDRelative susceptibility of microsomes from lung, heart, liver, kidney, brain and testes to lipid peroxidation: correlation with vitamin E content. Lipids 1980 15, 315–322.Google Scholar
32Koven, W, Barr, Y, Lutzky, S, Ben-Atia, I, Weiss, R, Harel, M, Behrens, P & Tandler, AThe effect of dietary arachidonic acid (20: 4 n -6) on growth, survival and resistance to handling stress in gilthead seabream (Sparus aurata) larvae. Aquaculture 2001 193, 107–122.Google Scholar
33Likimani, TA & Wilson, REffects of diet on lipogenic enzyme activities in channel catfish hepatic and adipose tissue. J Nutr 1982 112, 112–117.Google Scholar
34López-Bote, CJ, Diez, A, Corraze, G, Arzel, J, Alvarez, M, Dias, J, Kaushik, SJ & Bautista, JMDietary protein source affects the susceptibility to lipid peroxidation of rainbow trout (Oncorhynchus mykiss) and sea bass (Dicentrarchus labrax)muscle. Anim Sci 2001 73, 433–449.CrossRefGoogle Scholar
35Mckenzie, DJ, Higgs, DA, Dosanjh, B, Deacon, G & Randall, DJDietary lipid composition influences swimming performance in Atlantic salmon (Salmo salar) in seawater. Fish Physiol Biochem 1998 19, 111–122.CrossRefGoogle Scholar
36Menoyo, D, López-Bote, CJ, Bautista, JM & Obach, AHerring vs. anchovy fish oils in salmon feeding. Aquat Living Resour 2002 15, 217–223.CrossRefGoogle Scholar
37Menoyo, D, López-Bote, CJ, Bautista, JM & Obach, AGrowth, digestibility and fatty acid utilization in large Atlantic salmon Salmo salar fed varying levels of n−3 and saturated fatty acids. Aquaculture 2003 225, 295–307.CrossRefGoogle Scholar
38Monahan, FJ2000) Oxidation of lipids in muscle foods: fundamentals and applied concerns., In Antioxidants in Muscle Foods: Nutritional Strategies to Improve Quality, pp. 3–24 [Decker, EA, Faustman, and Lopez-Bote, CJeditors]. New York: Wiley Interscience 2000Google Scholar
39Montero, D, Kalinowski, T, Obach, A, Robaina, L, Tort, L, Caballero, MJ & Izquierdo, MSVegetable lipid sources for gilthead seabream (Sparus aurata): Effects on fish health. Aquaculture 2004(In the Press).Google Scholar
40Montero, D, Robaina, LE, Socorro, J, Vergara, JM, Tort, L & Izquierdo, MSAlteration of liver and muscle fatty acid composition in gilthead seabream (Sparus aurata) juveniles held a high stocking density and fed an essential fatty acid deficient diet. Fish Physiol Biochem 2001 24, 63–72.CrossRefGoogle Scholar
41Morris, TRExperimental Design and Analysis in Animal Sciences OxfordCAB International 1999.CrossRefGoogle Scholar
42Nanton, DA, Lall, SP, Ross, NW & McNiven, MAEffect of dietary lipid level on fatty acid β-oxidation and lipid composition in various tissues of haddock, Melanogrammus aeglefinus. Comp Biochem Physiol B 2003 135, 95–108.Google Scholar
43National Research Council Nutrient Requirements of Fish. Washington, DC: National Academy Press 1993Google Scholar
44Power, GW & Newsholme, EADietary fatty acids influence the activity and metabolic control of mitochondrial carnitine palmitoyltransferase I in rat heart and skeletal muscle. J Nutr 1997 127, 2142–2150.Google Scholar
45Regost, C, Arzel, J, Cardinal, M, Rosenlund, G & Kaushik, SJTotal replacement of fish oil by soybean or linseed oil with a return to fish oil in turbot (Psetta maxima) 2. Flesh quality properties. Aquaculture 2003 a 220, 737–747.Google Scholar
46Regost, C, Arzel, J, Robin, J, Rosenlund, G & Kaushik, SJ (2003b) Total replacement of fish oil by soybean or linseed oil with a return to fish oil in turbot (Psetta maxima) 1. Growth performance, flesh fatty acid profile, and lipid metabolism. Aquaculture 217, 465–482.Google Scholar
47Regost, C, Arzel, J, Robin, J, Rosenlund, G & Kaushik, SJTotal replacement of fish oil by soybean or linseed oil with a return to fish oil in turbot (Psetta maxima) 1. Growth performance, flesh fatty acid profile, and lipid metabolism. Aquaculture 2003 b 217, 465–482.CrossRefGoogle Scholar
48Robaina, L, Izquierdo, MS, Moyano, FJ, Socorro, J, Vergara, JM & Montero, DIncrease of the dietary n−3/n−6 fatty acid ratio and addition of phosphorous improves liver histological alterations induced by feeding diets containing soybean meal to gilthead seabream, Sparus aurata.. Aquaculture 1998 161, 281–293.Google Scholar
49Rørå, AMB, Regost, C & Lampe, JLiquid holding capacity, texture and fatty acid profile of smoked fillets of Atlantic salmon fed diets containing fish oil or soybean oil. Food Res Int 2003 36, 231–239.CrossRefGoogle Scholar
50Rosenlund, G, Obach, A, Sandberg, MG, Standal, H & Tveit, KEffect of alternative lipid sources on long-term growth perfomance and quality of Atlantic salmon (Salmo salar). Aquat Res 2001 32, 323–328.Google Scholar
51Sanz, M, Lopez-Bote, C, Menoyo, D & Bautista, JMAbdominal fat deposition and fatty acid synthesis are lower and β-oxidation is higher in broiler chickens fed diets containing unsaturated rather than saturated fat. J Nutr 2000 130, 3034–3037.CrossRefGoogle ScholarPubMed
52Shimeno, S, Ming, DC & Takeda, MMetabolic response to dietarycarbohydrate to lipid ratios in Oreochromis niloticus. Nippon Suisan Gakkai Shi 1993 59, 827–833.CrossRefGoogle Scholar
53Torstensen, BE, Lie, Ø & Frøyland, LLipid metabolism and tissue composition in Atlantic salmon (Salmo salar L) – Effects of capelin oil, palm oil, and oleic acid-enriched sunflower oil as dietary lipid sources. Lipids 2000 35, 653–664.Google Scholar
54Turchini, GM, Mentasti, T, Frøyland, L, Orban, E, Caprino, F, Moretti, VM & Valfré, FEffects of alternative dietary lipid sources on performance, tissue chemical composition, mitochondrial fatty acid oxidation capabilities and sensory characteristics in brown trout (Salmo trutta L.). Aquaculture 2003 225, 251–267.CrossRefGoogle Scholar
55Watanabe, T, Kitajima, C & Fujita, SNutritional values of live organisms used in Japan for mass propagation of fish: a review. Aquaculture 1983 34, 115–143.CrossRefGoogle Scholar
56Zammit, VAThe malonyl-CoA-long chain acyl-CoA axis in the maintenance of mammalian cell function. Biochem J 1999 343, 505–515.CrossRefGoogle ScholarPubMed