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The effect of calcium chloride injection on shear force and caspase activities in bovine longissimus muscles during postmortem conditioning

Published online by Cambridge University Press:  23 November 2011

J. Cao ,
X. Yu ,
M. A. Khan ,
J. Shao ,
Y. Xiang  and
G. Zhou
J. Cao
Affiliation:
College of Life Science and Biotechnology, Ningbo University, Ningbo 315211, P.R. China National Center of Meat Quality and Safety Control, Ministry of Science and Technology, Department of Food Science, Nanjing Agricultural University, Nanjing 210095, P.R. China
X. Yu
Affiliation:
Henan Institute of Science and Technology, College of Food Science and Technology, Xinxiang, Henan 453003, P.R. China
M. A. Khan
Affiliation:
National Center of Meat Quality and Safety Control, Ministry of Science and Technology, Department of Food Science, Nanjing Agricultural University, Nanjing 210095, P.R. China
J. Shao
Affiliation:
National Center of Meat Quality and Safety Control, Ministry of Science and Technology, Department of Food Science, Nanjing Agricultural University, Nanjing 210095, P.R. China
Y. Xiang
Affiliation:
Guizhou Rapeseed Institute, Guizhou Academy of Agricultural Sciences, Guiyang 550008, P.R. China
G. Zhou*
Affiliation:
National Center of Meat Quality and Safety Control, Ministry of Science and Technology, Department of Food Science, Nanjing Agricultural University, Nanjing 210095, P.R. China
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Abstract

Tenderness is considered as the most important quality determinant of meat. Calcium chloride application has been shown to improve tenderness by regulating endogenous proteinases. This study was designed to determine the effect of 300 mM calcium chloride injection on myofibrillar structures, caspase activities and shear force in longissimus muscles of bulls during postmortem storage of 7 days. Myofibrillar fragmentation index was determined as an index of proteolysis occurring in muscle fibers and associated proteins. Maximum tenderness was observed at days 4 and 7 in both treated and control samples. The injection of calcium chloride significantly increased myofibrillar proteolysis and improved tenderness at postmortem days 4 and 7. The treatment reduced caspase-9 activity at 4 h and day 4, whereas those of caspase-8 and -3 activities at days 1 and 4 with respect to control. The improved tenderness and increased myofibril fragmentation with decreased caspase activities suggested that the proteolytic systems activated with calcium chloride injection possibly behave independent of the caspase system.

Keywords

calcium chloridecaspasesconditioningproteolysistenderness

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Type
Full Paper
Information
animal , Volume 6 , Issue 6 , June 2012 , pp. 1018 - 1022
Copyright
Copyright © The Animal Consortium 2011

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