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Effect of Bacillus spp. direct-fed microbial on slurry characteristics and gaseous emissions in growing pigs fed with high fibre-based diets

Published online by Cambridge University Press:  14 July 2016

F. X. Prenafeta-Boldú ,
B. Fernández ,
M. Viñas ,
R. Lizardo ,
J. Brufau ,
A. Owusu-Asiedu ,
M. C. Walsh  and
A. Awati
F. X. Prenafeta-Boldú*
Affiliation:
GIRO Joint Research Unit IRTA-UPC, Torre Marimon, 08140 Caldes de Montbui, Barcelona, Spain
B. Fernández
Affiliation:
GIRO Joint Research Unit IRTA-UPC, Torre Marimon, 08140 Caldes de Montbui, Barcelona, Spain
M. Viñas
Affiliation:
GIRO Joint Research Unit IRTA-UPC, Torre Marimon, 08140 Caldes de Montbui, Barcelona, Spain
R. Lizardo
Affiliation:
IRTA–Mas de Bover, Ctra Reus-El Morell, Km 3,8. E-43120 Constantí, Tarragona, Spain
J. Brufau
Affiliation:
IRTA–Mas de Bover, Ctra Reus-El Morell, Km 3,8. E-43120 Constantí, Tarragona, Spain
A. Owusu-Asiedu
Affiliation:
Danisco Animal Nutrition, DuPont Industrial Biosciences, Marlborough SN8 1XN, UK Anpario Plc, Manton Wood Enterprise Park Worksop, Nottinghamshire S80 2RS, UK
M. C. Walsh
Affiliation:
Danisco Animal Nutrition, DuPont Industrial Biosciences, Marlborough SN8 1XN, UK
A. Awati
Affiliation:
Danisco Animal Nutrition, DuPont Industrial Biosciences, Marlborough SN8 1XN, UK
*
†E-mail: francesc.prenafeta@irta.cat
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Abstract

A 26-day trial with 18 Pietrain×(Landrace×Duroc) pigs was conducted to investigate the effect of two dose levels of a specifically selected Bacillus spp. direct-fed microbial (DFM) product, on the emission of environmentally harmful gasses (methane, ammonia and hydrogen sulphide) from manure. Pigs were assigned to one of three treatments in a randomized complete block design according to their sex and initial BW. Each treatment contained three replications with two pigs per pen. The test treatments included a Bacillus spp. DFM containing 3×108 colony-forming unit/g, added at a low (250 mg/kg) and high (500 mg/kg) dose to an antibiotic free high fibre-based diet, and a non-supplemented control diet. Manure from pigs fed with the supplemented diets emitted lower amounts of atmospheric contaminants. The most significant reduction was observed with low DFM supplementation, in which methane and ammonia volatilization decreased (P<0.05) by >40% and 50%, respectively, on fresh weight basis in relation to the control. Microbiome analysis of manure by high through put sequencing techniques on eubacterial and archaeal 16S rRNA genes highlighted the complex interactions between indigenous gut microflora and inoculated Bacillus spp. The tested Bacillus DFM could be considered as a best available technique in reducing the environmental impacts of growing pigs fed with high fibre-based diets.

Keywords

anaerobic digestionBacillusdirect-fed microbialsgaseous emissionspig slurry
Type
Research Article
Information
animal , Volume 11 , Issue 2 , February 2017 , pp. 209 - 218
Copyright
© The Animal Consortium 2016 

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