Hostname: page-component-7dd5485656-glrdx Total loading time: 0 Render date: 2025-10-25T04:28:26.388Z Has data issue: false hasContentIssue false
  • English
  • Français

Protein metabolism of rats treated with trienbolone acetate

Published online by Cambridge University Press:  02 September 2010

B. G. Vernon  and
P. J. Buttery
B. G. Vernon
Affiliation:
Department of Applied Biochemistry and Nutrition, University of Nottingham School of Agriculture, Sutton Bonington, Loughborough, Leicestershire LE12 5RD
P. J. Buttery
Affiliation:
Department of Applied Biochemistry and Nutrition, University of Nottingham School of Agriculture, Sutton Bonington, Loughborough, Leicestershire LE12 5RD
Get access

Abstract

(1) Both the anabolic agent trienbolone acetate (3-oxo-17-β-hydroxy- 4,9,11-estratriene acetate) and testosterone subcutaneously injected into entire female rats caused an increase in growth rate compared with placebo controls (P<0·01 and P<0·001 respectively). The trienbolone acetate rats grew slower than the testosterone-treated animals (P < 0·01). This may reflect differences in the mode of action of the steroids.

(2) In castrate males there were no significant differences in growth rate between the trienbolone acetate, testosterone or placebo control rats (P>0·05).

(3) Trienbolone acetate reduced the rate of myofibrillar protein degradation in female rats, within 3 days of treatment, at least as judged by Nt-methylhistidine excretion. The treated rats had superior nitrogen retentions (P<0·01) and feed conversions (P< 0·001).

(4) The data suggest that the rat is a suitable model on which to test the mode of action of trienbolone acetate.

Information

Type
Research Article
Information
Animal Production , Volume 26 , Issue 1 , February 1978 , pp. 1 - 9
Copyright
Copyright © British Society of Animal Science 1978

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

REFERENCES

Armstrong, N. G. 1966. Preparation of calcified and fossil hard tissues for amino acid analysis. In Techniques in Amino Acid Analysis (ed. Schmidt, D. I.) pp. 73–81. Technicon International Division S.A., Geneva.Google Scholar
Asatoor, A. M. and Armstrong, M. D. 1967. 3-methylhistidine, a component of actin. Biochem. biophys. Res. Commun. 26: 168–174.CrossRefGoogle ScholarPubMed
Association of Official Agricultural Chemists. 1970. Official Methods of Analysis. 11th ed. Association of Official Agricultural Chemists, Washington, DC.Google Scholar
Atkin, G. E. and Ferdinand, W. 1970. Accelerated amino acid analysis: studies on the use of lithium citrate buffers and the effect of n-propanol, in the analysis of physiological fluids and protein hydrolyzates. Analyt. Biochem. 38: 313–329.CrossRefGoogle ScholarPubMed
Best, J. M. J. 1972. The use of trienbolone acetate implants in heifer beef production at pasture. Vet. Rec. 91: 624–626.CrossRefGoogle ScholarPubMed
Brandon, D. L. 1976. The identification of myosin in rabbit hepatocytes. Eur. J. Biochem. 65: 139–146.CrossRefGoogle ScholarPubMed
Buttery, P. J. and Annison, E. F. 1973. Considerations of the efficiency of amino acid and protein metabolism in animals. In The Biological Efficiency of Protein Production (ed. Jones, J. W. G.), pp. 141–171. Cambridge University Press, London.Google Scholar
Chan, K. H., Heitzman, R. J. and Kitchenham, B. A. 1975. Digestibility and N-balance studies on growing heifers implanted with trienbolone acetate. Br. vet. J. 131: 170–174.CrossRefGoogle ScholarPubMed
Cowgill, R. A. and Freeburg, B. 1957. Metabolism of methylhistidine compounds in animals. Archs Biochem. Biophys. 71: 466–472.CrossRefGoogle ScholarPubMed
Gershey, E. L., Haslett, G. W., Vidali, G. and Allfrey, V. G. 1969. Chemical studies of histone methylation. Evidence for the occurrence of 3-methylhistidine in avian erythrocyte histone fractions. J. biol. Chem. 244: 4871–4877.CrossRefGoogle ScholarPubMed
Haverberg, L. N., Omstedt, P. T., Munro, H. N. and Young, V. R. 1975. W-methyl-histidine content of mixed proteins in various rat tissues. Biochim. biophys. Acta 405: 67–71.CrossRefGoogle ScholarPubMed
Heitzman, R. J. 1976. The effectiveness of anabolic agents in increasing rate of growth in farm animals; report on experiments in cattle. In Anabolic Agents in Animal Production (ed. Lu, F. C. and Rendel, J.), pp. 89–98. Thieme, Stuttgart.Google Scholar
Heitzman, R. J. and Chan, K. H. 1974. Alterations in weight gain and levels of plasma metabolites, protein, insulin and free fatty acids following implantation of an anabolic steroid in heifers. Br. vet. J. 130: 532–537.Google Scholar
Jelinek, J., Vesela, H. and Valova, B. 1964. The effect of nortestosterone phenyl-propionate on compensatory hypertrophy of the remaining kidney after unilateral nephrectomy. Acta endocr. (Kbh.) 46: 352–360.Google Scholar
Krüskemper, H.-L. 1968. Anabolic Steroids, pp. 31–87. Academic Press, New York.CrossRefGoogle Scholar
Lerner, L. J. 1964. Hormone antagonists: Inhibitors of specific activities of oestrogen and androgen. Recent Prog. Horm. Res. 20: 435–490.Google ScholarPubMed
Lobl, R. T. and Maenza, R. M. 1975. Androgenization: alteration in uterine growth and morphology. Biol. Reprod. 13: 255–268.CrossRefGoogle ScholarPubMed
Long, C. L., Haverberg, L. N., Young, V. R., Kinney, J. M., Munro, H. N. and Geiger, J. W. 1975. Metabolism of 3-methylhistidine in man. Metabolism 24: 929–935.Google Scholar
Mannan, A., Wiebe, L. I., Noujaim, A. A. and Second, D. C. 1975. Plasma free amino acids in the chronically anaemic rat. Clin. Biochem. 8: 303–306.CrossRefGoogle Scholar
Miller, S. A. 1969. Protein metabolism during growth and development. In Mammalian Protein Metabolism (ed. Munro, H. N.), vol. III, pp. 183–233. Academic Press, New York.CrossRefGoogle Scholar
Nishizawa, N., Funabiki, R. and Hareyama, S. 1975. Effects of dietary protein level on catabolic rates of myosin and actin in the rat revealed by urinary excretion of 3-methylhistidine. J. Nutr. Sci. Vitaminol. 21: 383–385.Google Scholar
Pottier, J., Busigny, M. and Grandadam, J. A. 1975. Plasma kinetics, excretion in milk and tissue levels in the cow following implantation with trienbolone acetate. J.Anim. Sci. 41: 962–968.CrossRefGoogle Scholar
Pousette, A. 1976. Demonstration of an androgen receptor in rat pancreas. Biochem. J. 157: 229–232.CrossRefGoogle ScholarPubMed
Ruh, T. S. and Ruh, M. F. 1975. Androgen induction of a specific uterine protein. Endocrinology 97: 1144–1150.CrossRefGoogle ScholarPubMed
Slob, A. K. and Van Der Werff Ten Bosch, J. J. 1975. Sex differences in body growth in the rat. Physiol. Behav. 14: 353–362.CrossRefGoogle ScholarPubMed
Trenkle, A. 1976. The anabolic effect of estrogens on nitrogen metabolism of growing and finishing cattle and sheep. In Anabolic Agents in Animal Production (ed. Lu, F. C. and Rendel, J.), pp. 79–88. Thieme, Stuttgart.Google Scholar
Vernon, B. G. and Buttery, P. J. 1976. Protein turnover in rats treated with trienbolone acetate. Br. J. Nutr. 36: 575–579.CrossRefGoogle ScholarPubMed
Waterlow, J. C. and Stephen, J. M. L. 1967. The measurement of total lysine turnover in the rat by intravenous infusion of L-[U-14C]lysine. Clin. Sci. 33: 489–506.Google Scholar
Young, V. R., Alexis, S. D., Baliga, B. S., Munro, H. N. and Muecke, N. 1972. Metabolism of administered 3-methylhistidine. Lack of muscle transfer ribonucleic acid charging and quantitative excretion as 3-methylhistidine and its N-acetyl derivative. J. biol. Chem. 247: 3592–3600.CrossRefGoogle ScholarPubMed