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Microbial contribution to duodenal purine flow in fattening cattle given concentrate diets, estimated by purine N labelling (15N) of different microbial fractions

Published online by Cambridge University Press:  18 August 2016

F. Vicente ,
J. A. Guada ,
J. Surra ,
J. Balcells  and
C. Castrillo
F. Vicente
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain
J. A. Guada*
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain
J. Surra
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain
J. Balcells
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain
C. Castrillo
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain
*
Corresponding author. E-mail:jguada@posta.unizar.es
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Abstract

The origin of duodenal purine bases (PB) was studied in a digestion experiment with four heifers, cannulated in the rumen and duodenum, which received a basal concentrate (152 g crude protein (CP) per kg dry matter (DM)) together with barley straw (85: 15 fresh weight basis) or the same concentrate supplemented with soya-bean meal, carbohydrate-treated soya-bean meal, maize gluten meal or fish meal to increase its protein content to 192 g/kg DM. Tr eatments were assigned to the four animals in five experimental periods according to an incomplete Latin-square design. Each 30-day period included 20 days of change-over adaptation and 10 days of experimental measurements. The flow of digesta entering the duodenum was estimated using Yb and acid-detergent insoluble ash as indigestible markers according to a double-marker system and microbial nitrogen (N) and PB were labelled with 15N infused into the rumen. The proportion of duodenal PB of microbial origin estimated from 15N enrichment of PB-N averaged 0·66 (s.e. 0·029) and did not differ between treatments nor when protozoa or bacteria associated with liquid (LAB) and solid (SAB) fractions were used as a reference sample. On average microbial contribution to duodenal non-ammonia N was higher when estimated from the PB/N ratio than from 15N (0·67 v. 0·55 (s.e. 0·015)) although differences were small and not significant when LAB was the reference sample (0·58 v. 0·52 (s.e. 0·018)) reflecting the higher PB/N ratio of this fraction compared with SAB and protozoa (2·04 v. 1·65 and 1·60 (s.e. 0·04) mmol/g). Considering only the duodenal PB of microbial origin resulted in estimates of microbial N synthesis from the PB/N ratio of SAB similar to those derived from 15N enrichment of both bacterial fractions (12·9 v. 13·5 and 13·3 (s.e. 0·83) g/kg of organic matter apparently digested in the rumen OMADR)) but underestimated the values derived from LAB (9·9 g/kg OMADR). Regardless of the estimation method, neither the duodenal flow of microbial N nor the efficiency of microbial synthesis differed between treatments. These results suggest that a significant proportion of duodenal PB have a non-microbial origin which may lead to overestimation of microbial yield when PB are used as a marker. Differences in PB/N ratio between microbial fractions is another important factor to be considered.

Keywords

cattlemarkersmicrobial proteinspurines

Information

Type
Ruminant nutrition, behaviour and production
Information
Animal Science , Volume 78 , Issue 1 , February 2004 , pp. 159 - 167
Copyright
Copyright © British Society of Animal Science 2004

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References

Agricultural and Food Research Council. 1993. Energy and protein requirements of ruminants. CAB International, Wallingford.Google Scholar
Agricultural Research Council. 1984. The nutrient requirements of ruminant livestock. Supplement no. 1. Commonwealth Agricultural Bureaux, Slough, England.Google Scholar
Aharoni, Y. and Tagari, H. 1991. Use of nitrogen-15 determinations of purine fractions of digesta to define nitrogen metabolism traits in the rumen. Journal of Dairy Science 74: 2540–2547.Google Scholar
Bates, D. B., Gillett, J. A., Barco, S. A. and Berger, W. G. 1985. The effect of specific growth rate and stage of growth on nucleic acid-protein values of pure cultures and mixed ruminal bacteria. Journal of Animal Science 61: 713–724.Google Scholar
Ben-Ghedalia, D.J, McMeniman, N. P. and Armstrong, D. G. 1978. The effect of partially replacing urea nitrogen with protein N on N capture in the rumen of sheep fed a purified diet. British Journal of Nutrition 39: 37–44.CrossRefGoogle Scholar
Calsamiglia, S., Stern, M. D. and Firkins, J. L. 1996. Comparison of nitrogen-15 and purines as microbial markers in continuous culture. Journal of Animal Science 74: 1375–1381.CrossRefGoogle ScholarPubMed
Carro, M. D. and Miller, E. L. 2002. Comparison of microbial markers (15N and purine bases) and bacterial isolates for the estimation of rumen microbial protein synthesis. Animal Science 75: 315–321.Google Scholar
Cecava, M. J., Merchen, N. R., Gay, L. C. and Berger, L. L. 1990. Composition of ruminal bacteria harvested from steers as influenced by dietary energy level, feeding frequency and isolation techniques. Journal of Dairy Science 73: 2480–2488.CrossRefGoogle ScholarPubMed
Chamberlain, D. G. and Thomas, P. C. 1979. Ruminal nitrogen metabolism and the passage of amino acids to the duodenum in sheep receiving diets containing hay and concentrates in various proportions. Journal of the Science of Food and Agriculture 30: 677–686.CrossRefGoogle Scholar
Craig, M. W., Brown, D. R., Broderick, A. and Ricker, D. B. 1987. Post-pandrial composition changes of fluid and particle-associated ruminal microorganism. Journal of Animal Science 65: 1024–1048.Google Scholar
Dehority, B. A. and Grubb, J. A. 1980. Effect of short-term chilling of rumen contents on viable bacterial number. Applied and Environmental Microbiology 39: 376–381.CrossRefGoogle Scholar
Djouvinov, D. S., Nakashima, Y., Todorov, N. and Pavlov, D. 1998 In situ degradation of feed purines. Animal Feed Science and Technology 71: 67–77.Google Scholar
Faichney, G. J. 1975. The use of markers to partition digestion within the gastrointestinal tract of ruminants. In Digestion and metabolism in the ruminant (ed. McDonald, I. W. and Warner, A.C. I.), pp. 277–291. University New England Publishing Unit, Armidale, New South Wales, Australia.Google Scholar
Goering, H. K. and Van Soest, P. J. 1970. Forage fiber analysis. Agricultural handbook no. 379, Agricultural Research Service, US Department of Agriculture, Washington DC.Google Scholar
Hvelplund, T. and Weisbjerg, M. R. 2000. In situ techniques for the estimation of protein degradability and postrumen availability. In Forage evaluation in ruminant nutrition (ed. Givens, D. I. Owens, E. Axford, R. F. E. and Omed, H. M.), pp. 233–258. CABI Publishing, Wallingford.Google Scholar
Jensen, E. S. 1991 Evaluation of automated analysis of 15N and total N in plant material and soil. Plant and Soil 133: 83–92.Google Scholar
John, A. and Ulyatt, M. J. 1984. Measurement of protozoa using phosphatidyl choline and of bacteria using nucleic acids in the duodenal digesta of sheep fed chaffed lucerne hay (Medicago sativa L.) diets. Journal of Agricultural Science, Cambridge 102: 33–44.Google Scholar
Lange, C. F. M.de, Souffrant, W. B. and Sauer, W. C. 1990. Real ileal protein and amino acid digestibliities in feedstuffs for growing pig as determined with the 15N-isotope dilution technique. Journal of Animal Science 68: 409–418.CrossRefGoogle ScholarPubMed
Legay-Carmier, F. and Bauchart, D. 1989. Distribution of bacteria in the rumen contents of dairy cows given a diet supplemented with soya-bean oil. British Journal of Nutrition 61: 725–740.Google Scholar
Ling, J. R. and Buttery, P. J. 1978. The simultaneous use of ribonucleic acid, 35S, 2, 6-diaminopimelic acid and 2-aminoethylphosphonic acid as markers of microbial nitrogen entering the duodenum of sheep. British Journal of Nutrition 39: 165–179.Google Scholar
McAllan, A. B. and Smith, R. H. 1973. Degradation of nucleic acids in the rumen. British Journal of Nutrition 29: 331–345.Google Scholar
Madsen, J. and Hvelplund, T. 1985. Protein degradation in the rumen. A comparison between in vivo, nylon bags, in vitro and buffer measurements. Acta Agriculturæ Scandinavica 25: (suppl. 1) 103–124.Google Scholar
Martin, C., Bernard, L. and Michalet-Doreau, B. 1996. Influence of sampling time and diet on amino acid composition of protozoal and bacterial fractions from bovine ruminal contents. Journal of Animal Science 74: 1157–1163.CrossRefGoogle ScholarPubMed
Martin, C., Williams, A. G. and Michalet-Doreau, B. 1994. Isolation and characteristics of the protozoal and bacterial fractions from bovine ruminal contents. Journal of Animal Science 72: 2962–2968.Google Scholar
Martín-Orúe, S. M., Balcells, J., Guada, J. A. and Castrillo, C. 1995. Endogenous purine and pyrimide derivative excretion in pregnant sows. British Journal of Nutrition 73: 375–385.Google Scholar
Martín-Orúe, S. M., Balcells, J., Guada, J. A. and Fondevila, M. 2000. Microbial nitrogen production in growing heifers: direct measurement of duodenal flow of purine bases versus urinary excretion of purine derivatives as estimation procedures. Animal Feed Science and Technology 88: 171–188.Google Scholar
Martín-Orúe, S. M., Balcells, J., Zakraoui, F. and Castrillo, C. 1998. Quantification and chemical composition of mixed bacteria harvested from solid fractions of rumen digesta: effect of detachment procedure. Animal Feed Science and Technology 71: 269–282.Google Scholar
Mayes, R. W., Dove, H., Chen, X. B. and Guada, J. A. 1995. Advances in the use of faecal and urinary markers for measuring diet composition, herbage intake and nutrient utilisation in herbivores. In Recent developments in the nutrition of herbivores (ed. Journet, M. Grenet, E. France, M. H. Theriez, M. and Demarquilly, C.), pp. 381–406. INRA Editions, Paris.Google Scholar
Merry, R. J. and McAllan, A. B. 1983. A comparison of chemical composition of mixed bacteria harvested from the liquid and solid fractions of rumen digesta. British Journal of Nutrition 50: 701–709.Google Scholar
Olubobokum, J. A., Craig, W. M. and Nipper, W. A. 1988. Characteristics of protozoal and bacterial fractions from micro organisms associated with ruminal fluid or particles. Journal of Animal Science 66: 2701–2710.Google Scholar
Ørskov, E. R., MacLeod, N. A. and Kyle, D. J. 1986. Flow of nitrogen from the rumen and abomasum in cattle and sheep given protein-free nutrients by intragastric infusion. British Journal of Nutrition 56: 241–248.Google Scholar
Owens, F. N. and Goetsch, A. L. 1986. Digesta passage and microbial protein synthesis. In Control of digestion and metabolism in ruminants (ed. Milligan, L. P. .L.Grovum, W and Dobson, A.), pp. 196–223. Prentice-Hall, Englewood Cliffs, New Jersey.Google Scholar
Pérez, J. F., Balcells, J., Guada, J. A. and Castrillo, C. 1997a. Contribution of dietary nitrogen and purine bases to the duodenal digesta: comparison of duodenal and polyester-bag measurements. Animal Science 65: 237–245.CrossRefGoogle Scholar
Pérez, J. F., Balcells, J., Guada, J. A. and Castrillo, C. 1997b. Rumen microbial production estimated either from urinary purine derivative excretion or from direct measurements of 15N and purine bases as microbial markers: effect of protein source and rumen bacteria isolates. Animal Science 65: 225–236.Google Scholar
Pérez, J. F., Rodriguez, C., Gonzalez, J., Balcells, J. and Guada, J. A. 1996. Contribution of dietary purine bases to duodenal digesta in sheep. In situ studies of purine degradability corrected for microbial contamination. Animal Feed Science and Technology 62: 251–262.Google Scholar
Razzaque, M. A., Topps, J. H., Kay, R. N. B. and Brockway, J. M. 1981. Metabolism of the nucleic acids of rumen bacteria by preruminant and ruminant lambs. British Journal of Nutrition 45: 517–527.Google Scholar
Rodríguez, C. A., González, J., Alvir, M. R., Reppeto, J. L., Centeno, C. and Lamrani, F. 2000. Composition of bacteria harvested from the liquid and solid fractions of the rumen of sheep as influenced by feed intake. British Journal of Nutrition 84: 369–376.Google Scholar
Siddons, R. C., Paradine, J., Breever, D. E. and Cornell, P. R. 1985. Ytterbium acetate as a particulate-phase digesta-flow marker. British Journal of Nutrition 54: 509–519.Google Scholar
Smith, R. H., McAllan, A. B., Hewitt, D. and Lewis, P. E. 1978. Estimation of amounts of microbial and dietary nitrogen compounds entering the duodenum of cattle. Journal of Agricultural Science, Cambridge 90: 557–568.Google Scholar
Statistical Analysis Systems Institute. 1988. SAS/STAT user’s guide, version 6.12. Statistical Analysis Systems Institute Inc., Cary, NC.Google Scholar
Storm, E. and Ørskov, E. R. 1983. The nutritive value of rumen microorganisms in ruminant. 1. Large-scale isolation and chemical composition of rumen microorganism. British Journal of Nutrition 50: 463–470.Google Scholar
Ushida, K., Jouany, J. P. and Lassalas, B. 1985. Determination of assay parameters for RNA analysis in bacterial and duodenal samples by espectrophotometry. Influence of sample treatment and preservation. Reproduction, Nutrition, Développement 25: 1037–1046.Google Scholar
Vega, A. de and Poppi, D. P. 1997. Extent of digestion and rumen condition as factors affecting passage of liquid and digesta particles in sheep. Journal of Agricultural Science, Cambridge 128: 207–215.Google Scholar
Vicente, F., Guada, J. A., Balcells, J. and Castrillo, C. 1999. Microbial contribution to the duodenal flow of purine bases in cattle. Proceedings of the British Society of Animal Science, 1999, p. 215 (abstr. )Google Scholar
Volden, H. and Harstad, O. M. 1998. Chemical composition of bacteria harvested from the rumen of dairy cows fed three diets differing in protein content and rumen protein degradability at two levels of intake. Acta Agriculturæ Scandinavica, Section A, Animal Science 48: 202–209.Google Scholar
Zinn, R. A. and Owens, F. N. 1982. Rapid procedure for quantifying nucleic acid content of digesta. In Protein requirements for cattle (ed. Owens, F. N.), pp. 26–30. Oklahoma State University, Stillwater.Google Scholar