Molecular Imaging with Reporter Genes

Sanjiv Sam Gambhir; Shahriar S. Yaghoubi

doi:10.1017/CBO9780511730405

Reporter genes have been used for several decades to study regulation of gene expression in vivo. However, it was little more than a decade ago that a new class of reporter genes was developed for imaging molecular events within living subjects. By following the interactions of protein molecules, researchers can resolve the complex chemical pathways that living cells utilise. This book focuses on this group of imaging reporter genes, starting with detailed descriptions of all reporter genes from different imaging modalities, including optical, MRI, and radionuclide-based imaging. Key scientists in the field explain how to enhance reporter gene imaging utility through instrumentation and the various applications of this technology. This is the first comprehensive book on all aspects of reporter gene imaging, detailing what is known in the field and future goals for research. Investigators in biomedical sciences, physicians, and the biotechnology and pharmaceutical industries will benefit from topics covered.

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Contents

Frontmatter
pp i-vi
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Contents
pp vii-viii
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Contributors
pp ix-xii
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Preface
pp xiii-xiv
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Part I - Types of Imaging Reporter Genes
pp 1-2
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1 - Fluorescent Reporter Proteins
pp 3-40
- By Robert E. Campbell, Michael W. Davidson
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2 - Revealing Biomolecular Mechanisms Through In Vivo Bioluminescence Imaging
pp 41-69
- By Timothy C. Doyle, Qian Wang, Christopher H. Contag
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3 - Reporter Gene Imaging with PET/SPECT
pp 70-87
- By June-Key Chung, Joo Hyun Kang, Keon Wook Kang
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4 - Noninvasive Imaging of Gene Expression with Magnetic Resonance Imaging and Magnetic Resonance Spectroscopy
pp 88-110
- By Mark D. Pagel, James P. Basilion
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Part II - Enhancing Reporter Gene Imaging Techniques
pp 111-112
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5 - Multimodality Imaging of Reporter Genes
pp 113-126
- By Pritha Ray, Sanjiv Sam Gambhir
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6 - Cell-Specific Imaging of Reporter Gene Expression Using a Two-Step Transcriptional Amplification Strategy
pp 127-148
- By Marxa L. Figueiredo, Sanjiv Sam Gambhir, Michael Carey, Lily Wu
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Part III - Imaging Instrumentations
pp 149-150
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7 - Physics, Instrumentation, and Methods for Imaging Reporter Gene Expression in Living Subjects
pp 151-192
- By Craig S. Levin
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Part IV - Current Applications of Imaging Reporter Genes
pp 193-194
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8 - Reporter Gene Imaging of Cell Signal Transduction
pp 195-226
- By Tarik F. Massoud, Ramasamy Paulmurugan, Carmel Chan, Hua Fan-Minogue, Sanjiv Sam Gambhir
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9 - Gene Therapy and Imaging of Transgene Expression in Living Subjects
pp 227-238
- By Shahriar S. Yaghoubi, Sanjiv Sam Gambhir
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10 - Imaging Regulation of Endogenous Gene Expression in Living Subjects
pp 239-257
- By Vladimir Ponomarev
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11 - Imaging Cell Trafficking and Immune Cell Activation Using PET Reporter Genes
pp 258-274
- By Evan D. Nair-Gill, Chengyi J. Shu, Isabel J. Hildebrandt, Dean O. Campbell, Owen N. Witte, Caius G. Radu
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12 - Imaging of Reporter Genes and Stem Cells
pp 275-296
- By Martin G. Rodriguez-Porcel, Sanjiv Sam Gambhir
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13 - Clinical Applications of Reporter Gene Technology
pp 297-314
- By Iván Peñuelas, Shahriar S. Yaghoubi, Felipe Prósper, Sanjiv Sam Gambhir
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Index
pp 315-321
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Molecular Imaging with Reporter Genes

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Contents

Frontmatter
pp i-vi

Contents
pp vii-viii

Contributors
pp ix-xii

Preface
pp xiii-xiv

Part I - Types of Imaging Reporter Genes
pp 1-2

1 - Fluorescent Reporter Proteins
pp 3-40

2 - Revealing Biomolecular Mechanisms Through In Vivo Bioluminescence Imaging
pp 41-69

3 - Reporter Gene Imaging with PET/SPECT
pp 70-87

4 - Noninvasive Imaging of Gene Expression with Magnetic Resonance Imaging and Magnetic Resonance Spectroscopy
pp 88-110

Part II - Enhancing Reporter Gene Imaging Techniques
pp 111-112

5 - Multimodality Imaging of Reporter Genes
pp 113-126

6 - Cell-Specific Imaging of Reporter Gene Expression Using a Two-Step Transcriptional Amplification Strategy
pp 127-148

Part III - Imaging Instrumentations
pp 149-150

7 - Physics, Instrumentation, and Methods for Imaging Reporter Gene Expression in Living Subjects
pp 151-192

Part IV - Current Applications of Imaging Reporter Genes
pp 193-194

8 - Reporter Gene Imaging of Cell Signal Transduction
pp 195-226

9 - Gene Therapy and Imaging of Transgene Expression in Living Subjects
pp 227-238

10 - Imaging Regulation of Endogenous Gene Expression in Living Subjects
pp 239-257

11 - Imaging Cell Trafficking and Immune Cell Activation Using PET Reporter Genes
pp 258-274

12 - Imaging of Reporter Genes and Stem Cells
pp 275-296

13 - Clinical Applications of Reporter Gene Technology
pp 297-314

Index
pp 315-321

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Molecular Imaging with Reporter Genes

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