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Chapter 6 - Laser-Assisted ICSI

Published online by Cambridge University Press:  02 December 2021

By
Roberta Maggiulli ,
Simona Alfano ,
Erminia Alviggi ,
Gemma Fabozzi ,
Danilo Cimadomo ,
Filippo Maria Ubaldi  and
Laura Rienzi
Edited by
Gianpiero D. Palermo  and
Zsolt Peter Nagy
Gianpiero D. Palermo
Affiliation:
Cornell Institute of Reproductive Medicine, New York
Zsolt Peter Nagy
Affiliation:
Reproductive Biology Associates, Atlanta, GA
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Summary

ICSI is a well-established insemination method in IVF due to its overall efficacy and reliability, even when severe male factor is not the main cause of infertility. Although conventional-ICSI provides high fertilization rate, it suffers from some technical drawbacks. Sudden or delayed oocyte degeneration may occur after ICSI even when performed by experienced embryologist and extreme care is applied during the injection phase. Preliminary results from studies conducted in early 2000s suggest that laser-assisted micro-drilling of the zona pellucida before injection (LA-ICSI) may be useful to make ICSI more successful when inseminating oocytes more prone to fertilization failure. Although this condition does not affect many couples, in case of degeneration of all the injected oocytes the IVF cycle must be cancelled. In these cases, LA-ICSI becomes a valuable option that deserves consideration.

Keywords

In vitro fertilizationintracytoplasmic sperm injectionlaser-assisted ICSIzona pellucidametaphase II oocyteoocyte qualityfailed fertilizationdegenerationembryosfragile oocytes.
Type
Chapter
Information
Manual of Intracytoplasmic Sperm Injection in Human Assisted Reproduction
With Other Advanced Micromanipulation Techniques to Edit the Genetic and Cytoplasmic Content of the Oocyte
 , pp. 59 - 65
Publisher: Cambridge University Press
Print publication year: 2021

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References

Rosenwaks, Z, Pereira, N. The pioneering of intracytoplasmic sperm injection: historical perspectives. Reproduction 2017;154:F71–7.CrossRefGoogle ScholarPubMed
Palermo, G, Joris, H, Devroey, P, Van Steirteghem, AC. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet 1992;340:17–18.CrossRefGoogle ScholarPubMed
Rosen, MP, Shen, S, Dobson, AT, Fujimoto, VY, McCulloch, CE, Cedars, MI. Oocyte degeneration after intracytoplasmic sperm injection: a multivariate analysis to assess its importance as a laboratory or clinical marker. Fertil Steril 2006;85:1736–43.CrossRefGoogle ScholarPubMed
ESHRE Special Interest Group of Embryology and Alpha Scientists in Reproductive Medicine. The Vienna consensus: report of an expert meeting on the development of ART laboratory performance indicators. Reprod Biomed Online 2017;35:494–510.CrossRefGoogle Scholar
Rubino, P, Vigano, P, Luddi, A, Piomboni, P. The ICSI procedure from past to future: a systematic review of the more controversial aspects. Hum Reprod Update 2016;22:194–227.Google Scholar
Palermo, GD, Alikani, M, Bertoli, M, Colombero, LT, Moy, F, Cohen, J, et al. Oolemma characteristics in relation to survival and fertilization patterns of oocytes treated by intracytoplasmic sperm injection. Hum Reprod 1996;11:172–6.CrossRefGoogle ScholarPubMed
Ebner, T, Yaman, C, Moser, M, Sommergruber, M, Jesacher, K, Tews, G. A prospective study on oocyte survival rate after ICSI: influence of injection technique and morphological features. J Assist Reprod Genet 2001;18:623–8.CrossRefGoogle ScholarPubMed
Nagy, ZP, Liu, J, Joris, H, Bocken, G, Desmet, B, Van Ranst, H, et al. The influence of the site of sperm deposition and mode of oolemma breakage at intracytoplasmic sperm injection on fertilization and embryo development rates. Hum Reprod 1995;10:3171–7.CrossRefGoogle ScholarPubMed
Palermo, GD, Cohen, J, Alikani, M, Adler, A, Rosenwaks, Z. Development and implementation of intracytoplasmic sperm injection (ICSI). Reprod Fertil Dev 1995;7:211–7; discussion 7–8.CrossRefGoogle ScholarPubMed
Mizobe, Y, Oya, N, Iwakiri, R, Yoshida, N, Sato, Y, Onoue, N, et al. Developmental ability of embryos produced from oocytes with fragile oolemma by intracytoplasmic sperm injection. J Assist Reprod Genet 2016;33:1685–90.CrossRefGoogle ScholarPubMed
Kimura, Y, Yanagimachi, R. Intracytoplasmic sperm injection in the mouse. Biol Reprod 1995;52:709–20.Google Scholar
Liu, J, Nagy, Z, Joris, H, Tournaye, H, Smitz, J, Camus, M, et al. Analysis of 76 total fertilization failure cycles out of 2732 intracytoplasmic sperm injection cycles. Hum Reprod 1995;10:2630–6.Google Scholar
Mantoudis, E, Podsiadly, BT, Gorgy, A, Venkat, G, Craft, IL. A comparison between quarter, partial and total laser assisted hatching in selected infertility patients. Hum Reprod 2001;16:2182–6.Google Scholar
Moser, M, Ebner, T, Sommergruber, M, Gaisswinkler, U, Jesacher, K, Puchner, M, et al. Laser-assisted zona pellucida thinning prior to routine ICSI. Hum Reprod 2004;19:573–8.CrossRefGoogle ScholarPubMed
Rienzi, L, Greco, E, Ubaldi, F, Iacobelli, M, Martinez, F, Tesarik, J. Laser-assisted intracytoplasmic sperm injection. Fertil Steril 2001;76:1045–7.CrossRefGoogle ScholarPubMed
Nagy, ZP, Oliveira, SA, Abdelmassih, V, Abdelmassih, R. Novel use of laser to assist ICSI for patients with fragile oocytes: a case report. Reprod Biomed Online 2002;4:27–31.Google Scholar
Abdelmassih, S, Cardoso, J, Abdelmassih, V, Dias, JA, Abdelmassih, R, Nagy, ZP. Laser-assisted ICSI: a novel approach to obtain higher oocyte survival and embryo quality rates. Hum Reprod 2002;17:2694–9.Google Scholar
Rienzi, L, Ubaldi, F, Martinez, F, Minasi, MG, Iacobelli, M, Ferrero, S, et al. Clinical application of laser-assisted ICSI: a pilot study. Eur J Obstet Gynecol Reprod Biol 2004;115 Suppl 1:S77-9.Google Scholar
Demirol, A, Benkhalifa, M, Sari, T, Gurgan, T. Use of laser-assisted intracytoplasmic sperm injection (ICSI) in patients with a history of poor ICSI outcome and limited metaphase II oocytes. Fertil Steril 2006;86:256–8.Google Scholar
Choi, KH, Lee, JH, Yang, YH, Yoon, TK, Lee, DR, Lee, WS. Efficiency of laser-assisted intracytoplasmic sperm injection in a human assisted reproductive techniques program. Clin Exp Reprod Med 2011;38:148–52.Google Scholar
Verza, S, Schneider, DT, Siqueira, S, Esteves, S. Laser-assisted intracytoplasmic sperm injection (LA-ICSI) is safe and less traumatic than conventional ICSI. Fertil Steril 2013;100:Supplement.Google Scholar
Richter, KS, Davis, A, Carter, J, Greenhouse, SJ, Mottla, GL, Tucker, MJ. No advantage of laser-assisted over conventional intracytoplasmic sperm injection: a randomized controlled trial [NCT00114725]. J Exp Clin Assist Reprod 2006;3:5.Google Scholar
Elhoussieny, A, El Mandooh, M, Bahaa-Eldin, A, Fathy, H. Laser assisted zona thinning versus the conventional mechanical method for intracytoplasmic sperm injection in ICSI programs. Int J Obstet Gynaecol Res 2016;3:221–39.Google Scholar
Davidson, LM, Liu, Y, Griffiths, T, Jones, C, Coward, K. Laser technology in the ART laboratory: a narrative review. Reprod Biomed Online 2019;38:725–39.CrossRefGoogle ScholarPubMed
Tannus, S, Son, WY, Gilman, A, Younes, G, Shavit, T, Dahan, MH. The role of intracytoplasmic sperm injection in non-male factor infertility in advanced maternal age. Hum Reprod 2017;32:119–24.Google Scholar
Furuhashi, K, Saeki, Y, Enatsu, N, Iwasaki, T, Ito, K, Mizusawa, Y, et al. Piezo-assisted ICSI improves fertilization and blastocyst development rates compared with conventional ICSI in women aged more than 35 years. Reprod Med Biol 2019;18:357–61.Google Scholar

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