Skip to main content Accessibility help

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
×
Hostname: page-component-745bb68f8f-b6zl4 Total loading time: 0 Render date: 2025-01-11T17:01:36.757Z Has data issue: false hasContentIssue false

49 - In Vitro Maturation of Human Oocytes

from PART III - ASSISTED REPRODUCTION

Published online by Cambridge University Press:  04 August 2010

By
Ezgi Demirtas ,
Hananel Holzer ,
Shai Elizur ,
Yariv Gidoni ,
Ri-Cheng Chian  and
Seang Lin Tan
Edited by
Botros R. M. B. Rizk ,
Juan A. Garcia-Velasco ,
Hassan N. Sallam  and
Antonis Makrigiannakis
Botros R. M. B. Rizk
Affiliation:
University of South Alabama
Juan A. Garcia-Velasco
Affiliation:
Rey Juan Carlos University School of Medicine,
Hassan N. Sallam
Affiliation:
University of Alexandria School of Medicine
Antonis Makrigiannakis
Affiliation:
University of Crete
Get access

Summary

DEVELOPMENT OF IN VITRO MATURATION

The history of in vitro maturation (IVM) of oocytes goes back to as far as the 1930s (1) to Pincus, Enzman, and Saunders who studied the maturation of mammalian oocytes including human oocytes in vivo and in vitro (1, 2). Pincus and his colleagues observed that when oocytes were removed from the follicles and cultured in the laboratory without hormones, they spontaneously resumed meiosis and progressed to the mature stage, metaphase II (MII), as they do in vivo. In 1965, Edwards (3, 4) described the kinetics of in vitro oocyte maturation both in animals – mice, sheep, cows, pigs, and rhesus monkeys – and in humans. He introduced tissue culture medium 199 (TCM-199) for IVM and showed that meiosis is resumed in 80 percent of immature oocytes independent of cycle day and gonadotropin support. He subsequently concluded that “human oocytes can be fertilized after maturation in-vitro.”

In 1983, Veeck et al. reported two pregnancies resulting from in vitro matured oocytes (5). These morphologically immature oocytes were recovered from human menopausal gonadotropin and/or follicle-stimulating hormone (FSH) and human chorionic gonadotropin (hCG) primed cycles in an in vitro fertilization (IVF) program.

Cha et al. published the first birth resulting from the fertilization of in vitro matured oocytes, which were harvested from an unstimulated ovary on cycle day 13 in an oocyte donation program (6). They also mentioned in the same report that immature oocytes were recovered from unstimulated ovaries both in proliferative and secretory phases.

Keywords

in vitro maturationintracytoplasmic sperm injectionoocyte maturationoocyte retrievalpolycystic ovary syndromeovarian hyperstimulation syndrome
Type
Chapter
Information
Infertility and Assisted Reproduction , pp. 448 - 455
Publisher: Cambridge University Press
Print publication year: 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Pincus, G, Enzmann, EV. The comparative behavior of mammalian eggs in vivo and in vitro. I. The activation of ovarian eggs. J Exp Med 1935;62:665–75.CrossRefGoogle ScholarPubMed
Pincus, G, Saunders, B. The comparative behavior of mammalian eggs in vivo and in vitro. VI. The maturation of human ovarian ova. Anat Rec 1939;75:537–45.CrossRefGoogle Scholar
Edwards, RG. Maturation in vitro of mouse, sheep, cow, pig, rhesus monkey and human ovarian oocytes. Nature 1965;208:349–51.CrossRefGoogle ScholarPubMed
Edwards, RG. Maturation in vitro of human ovarian oocytes. Lancet 1965;286:926–9.CrossRefGoogle Scholar
Veeck, LL, Wortham, JW Jr., Witmyer, J, et al. Maturation and fertilization of morphologically immature human oocytes in a program of in vitro fertilization. Fertil Steril 1983;39(5):594–602.CrossRefGoogle Scholar
Cha, KY, Koo, JJ, Ko, JJ, Choi, DH, Han, SY, Yoon, TK. Pregnancy after in vitro fertilization of human follicular oocytes collected from nonstimulated cycles, their culture in vitro and their transfer in a donor oocyte program. Fertil Steril 1991;55(1):109–13.CrossRefGoogle Scholar
Cha, KY, Do, BR, Chi, HJ, et al. Viability of human follicular oocytes collected from unstimulated ovaries and matured and fertilized in vitro. Reprod Fertil Dev 1992;4(6):697–701.CrossRefGoogle Scholar
Trounson, A, Wood, C, Kausche, A. In vitro maturation and the fertilization and developmental competence of oocytes recovered from untreated polycystic ovarian patients. Fertil Steril 1994;62(2):353–62.CrossRefGoogle ScholarPubMed
Barnes, FL, Crombie, A, Gardner, DK, et al. Blastocyst development and birth after in-vitro maturation of human primary oocytes, intracytoplasmic sperm injection and assisted hatching. Hum Reprod 1995;10(12):3243–7.CrossRefGoogle ScholarPubMed
Steptoe, PC, Edwards, RG. Birth after the reimplantation of a human embryo. Lancet 1978;2(8085):366.CrossRefGoogle ScholarPubMed
Tan, SL, Royston, P, Campbell, S, et al. Cumulative conception and livebirth rates after in-vitro fertilisation. Lancet 1992; 339(8806):1390–4.CrossRefGoogle ScholarPubMed
MacDougall, MJ, Tan, SL, Jacobs, HS. In-vitro fertilization and the ovarian hyperstimulation syndrome. Hum Reprod 1992;7(5): 597–600.CrossRefGoogle ScholarPubMed
MacDougall, MJ, Tan, SL, Balen, A, Jacobs, HS. A controlled study comparing patients with and without polycystic ovaries undergoing in-vitro fertilization. Hum Reprod 1993;8(2):233–7.CrossRefGoogle ScholarPubMed
Buckett, W, Chian, RC, Tan, SL. Can we eliminate severe ovarian hyperstimulation syndrome? Not completely. Hum Reprod 2005;20(8):2367; author reply 8.CrossRefGoogle Scholar
Murray, C, Golombok, S. Oocyte and semen donation: a survey of UK licensed centres. Hum Reprod 2000;15(10):2133–9.CrossRefGoogle ScholarPubMed
Thomas, FH, Vanderhyden, BC. Oocyte growth and developmental competence. In: Tan, SL, Chian, RC, Buckett, WM, eds. In-vitro Maturation of Human Oocytes, Basic Science to Clinical Application. London: Informa Healthcare; 2007:1–14.Google Scholar
Cha, KY, Chian, RC. Maturation in vitro of immature human oocytes for clinical use. Hum Reprod Update 1998;4(2):103–20.CrossRefGoogle ScholarPubMed
Swain, JE, Smith, GD. Mechanism of oocyte maturation. In: Tan, SL, Chian, RC, Buckett, WM, eds. In-vitro Maturation of Human Oocytes, Basic Science to Clinical Application. London: Informa Healthcare; 2007:83–12.Google Scholar
Chian, RC, Buckett, WM, Tan, SL. In-vitro maturation of human oocytes. Reprod Biomed Online 2004;8(2):148–66.CrossRefGoogle ScholarPubMed
Chian, RC, Sirard, MA. Effects of cumulus cells and follicle- stimulating hormone during in vitro maturation on parthenogenetic activation of bovine oocytes. Mol Reprod Dev 1995;42(4):425–31.CrossRefGoogle ScholarPubMed
Chian, RC, Buckett, WM, Too, LL, Tan, SL. Pregnancies resulting from in vitro matured oocytes retrieved from patients with polycystic ovary syndrome after priming with human chorionic gonadotropin. Fertil Steril 1999;72(4):639–42.CrossRefGoogle ScholarPubMed
Chian, RC, Buckett, WM, Tulandi, T, Tan, SL. Prospective randomized study of human chorionic gonadotrophin priming before immature oocyte retrieval from unstimulated women with polycystic ovarian syndrome. Hum Reprod 2000;15(1):165–70.CrossRefGoogle ScholarPubMed
Mikkelsen, AL, Lindenberg, S. Influence of the dominant follicle on in-vitro maturation of human oocytes: a prospective non-randomized study. Reprod Biomed Online 2001;3(3):199–204.CrossRefGoogle ScholarPubMed
Mikkelsen, AL, Host, E, Blaabjerg, J, Lindenberg, S. Maternal serum supplementation in culture medium benefits maturation of immature human oocytes. Reprod Biomed Online 2001;3(2):112–16.CrossRefGoogle ScholarPubMed
Trounson, A, Anderiesz, C, Jones, G. Maturation of human oocytes in vitro and their developmental competence. Reproduction 2001;121(1):51–75.CrossRefGoogle ScholarPubMed
Trounson, A, Anderiesz, C, Jones, GM, Kausche, A, Lolatgis, N, Wood, C. Oocyte maturation. Hum Reprod 1998;13(Suppl. 3):52–62.CrossRefGoogle ScholarPubMed
Chian, RC, Tan, SL. Maturational and developmental competence of cumulus-free immature human oocytes derived from stimulated and intracytoplasmic sperm injection cycles. Reprod Biomed Online 2002;5(2):125–32.CrossRefGoogle ScholarPubMed
Tesarik, J, Mendoza, C. Nongenomic effects of 17 beta-estradiol on maturing human oocytes: relationship to oocyte developmental potential. J Clin Endocrinol Metab 1995;80(4):1438–43.Google ScholarPubMed
Kreiner, D, Liu, HC, Itskovitz, J, Veeck, L, Rosenwaks, Z. Follicular fluid estradiol and progesterone are markers of preovulatory oocyte quality. Fertil Steril 1987;48(6):991–4.CrossRefGoogle ScholarPubMed
Chian, RC, Ao, A, Clarke, HJ, Tulandi, T, Tan, SL. Production of steroids from human cumulus cells treated with different concentrations of gonadotropins during culture in vitro. Fertil Steril 1999;71(1):61–6.CrossRefGoogle ScholarPubMed
Cobo, AC, Requena, A, Neuspiller, F, et al. Maturation in vitro of human oocytes from unstimulated cycles: selection of the optimal day for ovum retrieval based on follicular size. Hum Reprod 1999;14(7):1864–8.CrossRefGoogle ScholarPubMed
Chian, RC, Lim, JH, Tan, SL. State of the art in in-vitro oocyte maturation. Curr Opin Obstet Gynecol 2004;16(3):211–19.CrossRefGoogle ScholarPubMed
Paulson, RJ, Sauer, MV, Francis, MM, Macaso, T, Lobo, RA. Factors affecting pregnancy success of human in-vitro fertilization in unstimulated cycles. Hum Reprod 1994;9(8):1571–5.CrossRefGoogle ScholarPubMed
Thornton, MH, Francis, MM, Paulson, RJ. Immature oocyte retrieval: lessons from unstimulated IVF cycles. Fertil Steril 1998; 70(4):647–50.CrossRefGoogle ScholarPubMed
Smith, LC, Olivera-Angel, M, Groome, NP, Bhatia, B, Price, CA. Oocyte quality in small antral follicles in the presence or absence of a large dominant follicle in cattle. J Reprod Fertil 1996; 106(2):193–9.CrossRefGoogle ScholarPubMed
Chian, RC, Chung, JT, Downey, BR, Tan, SL. Maturational and developmental competence of immature oocytes retrieved from bovine ovaries at different phases of folliculogenesis. Reprod Biomed Online 2002;4(2):127–32.CrossRefGoogle ScholarPubMed
Bomsel-Helmreich, O, Huyen, LV, Durand-Gasselin, I, Salat- Baroux, J, Antoine, JM. Mature and immature oocytes in large and medium follicles after clomiphene citrate and human menopausal gonadotropin stimulation without human chorionic gonadotropin. Fertil Steril 1987;48(4):596–604.CrossRefGoogle ScholarPubMed
Nagy, ZP, Cecile, J, Liu, J, Loccufier, A, Devroey, P, Steirteghem, A. Pregnancy and birth after intracytoplasmic sperm injection of in vitro matured germinal-vesicle stage oocytes: case report. Fertil Steril 1996;65(5):1047–50.CrossRefGoogle ScholarPubMed
Edirisinghe, WR, Junk, SM, Matson, PL, Yovich, JL. Birth from cryopreserved embryos following in-vitro maturation of oocytes and intracytoplasmic sperm injection. Hum Reprod 1997;12(5): 1056–8.CrossRefGoogle ScholarPubMed
Liu, J, Katz, E, Garcia, JE, Compton, G, Baramki, TA. Successful in vitro maturation of human oocytes not exposed to human chorionic gonadotropin during ovulation induction, resulting in pregnancy. Fertil Steril 1997;67(3):566–8.CrossRefGoogle Scholar
Jaroudi, KA, Hollanders, JM, Elnour, AM, Roca, GL, Atared, AM, Coskun, S. Embryo development and pregnancies from in-vitro matured and fertilized human oocytes. Hum Reprod 1999;14(7):1749–51.CrossRefGoogle ScholarPubMed
Lim, KS, Son, WY, Yoon, SH, Lim, JH. IVM/F-ET in stimulated cycles for the prevention of OHSS. Fertil Steril 2002;78(Suppl. 1):S10.CrossRefGoogle Scholar
Suikkari, AM, Tulppala, M, Tuuri, T, Hovatta, O, Barnes, F. Luteal phase start of low-dose FSH priming of follicles results in an efficient recovery, maturation and fertilization of immature human oocytes. Hum Reprod 2000;15(4):747–51.CrossRefGoogle Scholar
Mikkelsen, AL. FSH priming in IVM cycles. In: Tan, SL, Chian, RC, Buckett, W, eds. In-vitro Maturation of Human Oocytes, Basic Science to Clinical Application. London: Informa Healthcare; 2007.Google Scholar
Yang, SH, Son, WY, Yoon, SH, Ko, Y, Lim, JH. Correlation between in vitro maturation and expression of LH receptor in cumulus cells of the oocytes collected from PCOS patients in HCG-primed IVM cycles. Hum Reprod 2005;20(8):2097–103.CrossRefGoogle ScholarPubMed
Chian, RC, Gulekli, B, Buckett, WM, Tan, SL. Priming with human chorionic gonadotropin before retrieval of immature oocytes in women with infertility due to the polycystic ovary syndrome. N Engl J Med 1999;341(21):1624, 6.CrossRefGoogle ScholarPubMed
Gulekli, B, Buckett, WM, Chian, RC, Child, TJ, Abdul-Jalil, AK, Tan, SL. Randomized, controlled trial of priming with 10,000 IU versus 20,000 IU of human chorionic gonadotropin in women with polycystic ovary syndrome who are undergoing in vitro maturation. Fertil Steril 2004;82(5):1458–9.CrossRefGoogle ScholarPubMed
Lin, YH, Hwang, JL, Huang, LW, et al. Combination of FSH priming and hCG priming for in-vitro maturation of human oocytes. Hum Reprod 2003;18(8):1632–6.CrossRefGoogle ScholarPubMed
Mikkelsen, AL, Smith, S, Lindenberg, S. Impact of oestradiol and inhibin A concentrations on pregnancy rate in in-vitro oocyte maturation. Hum Reprod 2000;15(8):1685–90.CrossRefGoogle ScholarPubMed
Child, TJ, Gulekli, B, Tan, SL. Success during in-vitro maturation (IVM) of oocyte treatment is dependent on the numbers of oocytes retrieved which are predicted by early follicular phase transvaginal ultrasound measurement of the antral follicular count and peak ovarian stromal blood flow velocity. Hum Reprod 2001;16(Abstract book 1):41.Google Scholar
Tan, SL, Child, TJ, Gulekli, B. In vitro maturation and fertilization of oocytes from unstimulated ovaries: predicting the number of immature oocytes retrieved by early follicular phase ultrasonography. Am J Obstet Gynecol 2002;186(4):684–9.CrossRefGoogle ScholarPubMed
Child, TJ, Gulekli, B, Chian, RC, Abdul Jalil, AK, Tan, SL. In-vitro maturation of oocytes from unstimulated normal ovaries of women with a previous poor response to IVF. Fertil Steril 2000;74(3 Suppl. 1):S45.Google Scholar
Liu, J, Lu, G, Qian, Y, Mao, Y, Ding, W. Pregnancies and births achieved from in vitro matured oocytes retrieved from poor responders undergoing stimulation in in vitro fertilization cycles. Fertil Steril 2003;80(2):447–9.CrossRefGoogle ScholarPubMed
Nargund, G, Waterstone, J, Bland, J, Philips, Z, Parsons, J, Campbell, S. Cumulative conception and live birth rates in natural (unstimulated) IVF cycles. Hum Reprod 2001;16(2):259–62.CrossRefGoogle ScholarPubMed
Chian, RC, Buckett, WM, Abdul Jalil, AK, et al. Natural-cycle in vitro fertilization combined with in vitro maturation of immature oocytes is a potential approach in infertility treatment. Fertil Steril 2004;82(6):1675–8.CrossRefGoogle ScholarPubMed
Lim, JH, Park, SY, Yoon, SH, Yang, SH, Chian, RC. Combination of natural cycle IVF with IVM as infertility treatment. In: Tan, SL, Chian, RC, Buckett, W, eds. In-Vitro Maturation of Oocytes, Basic Science to Clinical Application. London: Informa Healthcare; 2007.Google Scholar
Holzer, H, Scharf, E, Chian, RC, Demirtas, E, Buckett, W, Tan, SL. In vitro maturation of oocytes collected from unstimulated ovaries for oocyte donation. Fertil Steril 2007. In press.CrossRefGoogle ScholarPubMed
Hayat, MJ, Howlader, N, Reichman, ME, Edwards, BK. Cancer statistics, trends, and multiple primary cancer analyses from the Surveillance, Epidemiology, and End Results (SEER) Program. Oncologist 2007;12(1):20–37.CrossRefGoogle ScholarPubMed
Lee, SJ, Schover, LR, Partridge, AH, et al. American Society of Clinical Oncology recommendations on fertility preservation in cancer patients. J Clin Oncol 2006;24(18):2917–31.CrossRefGoogle Scholar
Rao, GD, Chian, RC, Son, WS, Gilbert, L, Tan, SL. Fertility preservation in women undergoing cancer treatment. Lancet 2004;363(9423):1829–30.CrossRefGoogle ScholarPubMed
Holzer, HE, Tan, SL. Fertility preservation in oncology. Minerva Ginecol 2005;57(1):99–109.Google Scholar
Oktay, K, Demirtas, E, Son, W, Lostritto, K, Chian, RC, Tan, SL. In vitro maturation of germinal vesicle oocytes recovered post- premature LH surge: description of a novel approach to fertility preservation. Fertil Steril 2007. In press.Google Scholar
Huang, JYJ, Holzer, H, Tulandi, T, Tan, SL, Chian, RC. Combining ovarian tissue cryobanking with retrieval of immature oocytes by in vitro maturation and vitrification: a noble method of fertility preservation. Fertil Steril 2007. In press.Google Scholar
Child, TJ, Abdul-Jalil, AK, Gulekli, B, Tan, SL. In vitro maturation and fertilization of oocytes from unstimulated normal ovaries, polycystic ovaries, and women with polycystic ovary syndrome. Fertil Steril 2001;76(5):936–42.CrossRefGoogle ScholarPubMed
Child, TJ, Phillips, SJ, Abdul-Jalil, AK, Gulekli, B, Tan, SL. A comparison of in vitro maturation and in vitro fertilization for women with polycystic ovaries. Obstet Gynecol 2002;100(4):665–70.Google ScholarPubMed
Assisted reproductive technology in the United States: 2001 results generated from the American Society for Reproductive Medicine/Society for Assisted Reproductive Technology registry. Fertil Steril 2007.
Gunby, J, Daya, S. Assisted reproductive technologies (ART) in Canada: 2003 results from the Canadian ART Register. Fertil Steril 2007.CrossRefGoogle ScholarPubMed
Mikkelsen, AL, Ravn, SH, Lindenberg, S. Evaluation of newborns delivered after in vitro maturation. Hum Reprod 2003;18(Suppl. 1):xviii5.Google Scholar
Buckett, WM, Chian, RC, Barrington, K, Dean, N, Abdul Jalil, AK, Tan, SL. Obstetric, neonatal and infant outcome in babies conceived by in vitro maturation (IVM): initial five-year results 1998–2003. Fertil Steril 2004;82(Suppl. 2):S133.CrossRefGoogle Scholar
Cha, KY, Chung, HM, Lee, DR, et al. Obstetric outcome of patients with polycystic ovary syndrome treated by in vitro maturation and in vitro fertilization-embryo transfer. Fertil Steril 2005;83(5):1461–5.CrossRefGoogle ScholarPubMed
Buckett, WM. Pregnancy and neonatal outcome following IVM. In: Tan, SL, Chian, RC, Buckett, WM, eds. In-vitro Maturation of Human Oocytes, Basic Science to Clinical Application. London: Informa Healthcare; 2007:313–18.Google Scholar
Hansen, M, Kurinczuk, JJ, Bower, C, Webb, S. The risk of major birth defects after intracytoplasmic sperm injection and in vitro fertilization. N Engl J Med 2002;346(10):725–30.CrossRefGoogle ScholarPubMed
Buckett, W, Chian, RC, Holzer, H, Dean, N, Tan, SL. Congenital abnormalities and perinatal outcome in pregnancies following IVM, IVF, and ICSI delivered in a single center. Fertil Steril 2006;85(Suppl. 2):S11–12.CrossRefGoogle Scholar

Save book to Kindle

To save this book to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Available formats
×

Save book to Dropbox

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Dropbox.

Available formats
×

Save book to Google Drive

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Google Drive.

Available formats
×